FlyBase Allele Report: Dmel\jeb[UAS.cVa]

General Information

Symbol

Dmel\jeb^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Varshney

FlyBase ID

FBal0194694

Feature type

allele

Associated gene

Dmel\jeb

Associated Insertion(s)

Carried in Construct

P{UAS-jeb.V}

Also Known As

UAS-jeb

Key Links

Transgenic product class

cDNA

(Varshney and Palmer, 2006)

wild_type

(Varshney and Palmer, 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Varshney and Palmer, 2006)

wild_type

(Varshney and Palmer, 2006)

Mutagen

in vitro construct

(Bazigou et al., 2007, Bazigou et al., 2007, Varshney and Palmer, 2006)

Progenitor genotype

Carried in construct

P{UAS-jeb.V}

Cytology

Description

UASt regulatory sequences drive expression of a jeb cDNA.

(Varshney and Palmer, 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

jeb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^386Y

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Alk.38

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^elav-C155

(Wolfstetter et al., 2017, Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

decreased body size | P-stage, with Scer\GAL4^elav-C155

(Sukumar et al., 2024, Pfeifer et al., 2022)

decreased body size | pupal stage, with Scer\GAL4^elav-C155

(Hugosson et al., 2014)

increased cell number | larval stage, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

viable, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

Phenotype Manifest In

circular trunk visceral muscle primordium, with Scer\GAL4^bap.3

(Wolfstetter et al., 2009)

larval brain | larval stage, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

larval neuron | increased number | larval stage, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

pupa, with Scer\GAL4^elav-C155

(Pfeifer et al., 2022)

Detailed Description

Statement

Reference

Expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155 results in markedly decreased pupal size.

(Wolfstetter et al., 2017)

Pan-neuronal expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^Ras64B.PW or Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of jeb^Scer\UAS.cVa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, does not affect associative olfactory learning.

Pan-neuronal expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155 throughout development yields pupae that are 10-15% smaller than controls. The resulting adults are viable and normally patterned. Smaller pupae are also seen when jeb^Scer\UAS.cVa is expressed under the control of Scer\GAL4^Ras64B.PW, Scer\GAL4^Alk.38 or Scer\GAL4^386Y.

Expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^Ilp2.PR throughout development yields pupae that are a similar size to controls.

(Gouzi et al., 2011)

Embryos expressing jeb^Scer\UAS.cVa under the control of Scer\GAL4^bap.3 show an increase of circular visceral founder cells at the expense of visceral fusion competent myoblasts. The midgut primordial cells are able to contact the visceral mesoderm and show proper migration in these embryos.

(Wolfstetter et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, jeb^UAS.cVa has abnormal size | pupal stage phenotype, suppressible | partially by cnk[+]/cnk^k16314

(Wolfstetter et al., 2017)

Scer\GAL4^elav-C155, jeb^UAS.cVa has abnormal size | pupal stage phenotype, suppressible | partially by cnk[+]/cnk^63F

(Wolfstetter et al., 2017)

Scer\GAL4^elav-C155, jeb^UAS.cVa has abnormal size | pupal stage phenotype, suppressible | partially by rl[+]/rl^10a

(Wolfstetter et al., 2017)

Enhancer of

Statement

Reference

jeb^UAS.cVa/Scer\GAL4^elav-C155 is an enhancer of abnormal size | pupal stage | dominant phenotype of Nf1^E2

(Gouzi et al., 2011)

Other

Statement

Reference

Nf1^E2, Scer\GAL4^elav-C155, jeb^UAS.cVa has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Nf1^E2, Scer\GAL4^Ras64B.PW, jeb^UAS.cVa has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Nf1^E2, Scer\GAL4^386Y, jeb^UAS.cVa has lethal | recessive | larval stage phenotype

(Gouzi et al., 2011)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The reduced pupal size characteristic for animals expressing jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155 is partially suppressed by combination with a single copy of any of the following: cnk^k16314, cnk^63F or rl^10a.

(Wolfstetter et al., 2017)

Expression of jeb^Scer\UAS.cVa under the control of Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW or Scer\GAL4^386Y in a Nf1^E2 mutant background results in larval lethality. In a Nf1^E2/+ background pupae are able to develop and the small size phenotype is partially suppressed.

(Gouzi et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

jeb^UAS.cVa/Scer\GAL4^en-e16E rescues jeb^weli/Df(2R)BSC199

(Hugosson et al., 2014)

jeb^UAS.cVa/Scer\GAL4^GMR.PF rescues jeb^SH0422

(Bazigou et al., 2007)

Comments

Scer\GAL4^en-e16E-mediated expression of jeb^Scer\UAS.cVa rescues visceral mesoderm formation in jeb^weli/Df(2R)BSC199 embryos.

(Hugosson et al., 2014)

R cell projection defects in flies carrying jeb^SH0422 clones in the eye are rescued by expression of jeb^Scer\UAS.cPa under the control of Scer\GAL4^GMR.PF.

(Bazigou et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

jeb^Scer\UAS.cPa

jeb^Scer\UAS.cVa

jeb^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Palmer

Saccharomyces cerevisiae UAS construct a of Varshney

Secondary FlyBase IDs

References (16)

Report Sections

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