FB2021_01, released Feb 18, 2021

Allele: Dmel\DebcldsRNA.UAS

Open Close
General Information
Symbol
Dmel\DebcldsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0194703
Feature type
allele
Associated gene
Dmel\Debcl
Associated Insertion(s)
Carried in Construct
P{UAS-Debcl.dsRNA}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Senoo-Matsuda et al., 2005)
in vitro construct - RNAi
(Senoo-Matsuda et al., 2005)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Debcl.dsRNA}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UAS sequences drive expression of a 500bp-long Debcl cDNA inverted repeat (IR). The IR is in a head-to-head orientation.

      (Senoo-Matsuda et al., 2005)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      Debcl
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Senoo-Matsuda et al., 2005)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of the debcldsRNA.Scer\UAS transgene, driven by Scer\GAL4GMR.PF, completely blocks the small-eye phenotype caused by debclScer\UAS.T:Ivir\HA1 overexpression.

      Embryos in which ubiquitous expression of the debcldsRNA.Scer\UAS transgene is driven by Scer\GAL4da.G32 show a remarkable reduction in the number of dying cells compared with controls.

      debcldsRNA.Scer\UAS expression targeted to the developing retina or photoreceptor neurons using Scer\GAL4GMR.PF or Scer\GAL4sev.EP has no effect on eye morphology.

      The lifespans of flies expressing debcldsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 are markedly shorter than those of control flies. Additionally, the frequency of locomotion in the Scer\GAL4elav-C155; debcldsRNA.Scer\UAS flies is significantly reduced compared with that of control flies.

      The ATP levels in flies expressing debcldsRNA.Scer\UAS, under the control of Scer\GAL4hs.PB following a heat shock, are significantly lower than those in control fly lines.

      (Senoo-Matsuda et al., 2005)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Flies expressing both Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 and debclScer\UAS.T:Ivir\HA1 in their neurons, under the control of Scer\GAL4elav-C155, show a significantly shorter lifespan than flies expressing Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 alone.

      Coexpression of Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 and debclScer\UAS.T:Ivir\HA1, driven by Scer\GAL4GMR.PF, accelerates the onset of eye neurodegeneration seen in adults expressing Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1. The loss of retinal structure and pigment defects are strongly enhanced by debclScer\UAS.T:Ivir\HA1 expression.

      Eyes coexpressing debclScer\UAS.T:Ivir\HA1, BuffyScer\UAS.cQa, and Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 (all driven by Scer\GAL4GMR.PF) show a significantly weaker degenerative eye phenotype than eyes expressing BuffyScer\UAS.cQa and Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1.

      (Senoo-Matsuda et al., 2005)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      dsRNA has been transfected into embryos to study the phenotypic consequences of dsRNA interference (RNAi) of the debcl gene.

      (Senoo-Matsuda et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      DebcldsRNA.Scer\UAS
      DebcldsRNA.UAS
      debcldsRNA.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)