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General Information
Symbol
Dmel\DebcldsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0194703
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of a 500bp-long Debcl cDNA inverted repeat (IR). The IR is in a head-to-head orientation.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of the debcldsRNA.Scer\UAS transgene, driven by Scer\GAL4GMR.PF, completely blocks the small-eye phenotype caused by debclScer\UAS.T:Ivir\HA1 overexpression.

Embryos in which ubiquitous expression of the debcldsRNA.Scer\UAS transgene is driven by Scer\GAL4da.G32 show a remarkable reduction in the number of dying cells compared with controls.

debcldsRNA.Scer\UAS expression targeted to the developing retina or photoreceptor neurons using Scer\GAL4GMR.PF or Scer\GAL4sev.EP has no effect on eye morphology.

The lifespans of flies expressing debcldsRNA.Scer\UAS under the control of Scer\GAL4elav-C155 are markedly shorter than those of control flies. Additionally, the frequency of locomotion in the Scer\GAL4elav-C155; debcldsRNA.Scer\UAS flies is significantly reduced compared with that of control flies.

The ATP levels in flies expressing debcldsRNA.Scer\UAS, under the control of Scer\GAL4hs.PB following a heat shock, are significantly lower than those in control fly lines.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Flies expressing both Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 and debclScer\UAS.T:Ivir\HA1 in their neurons, under the control of Scer\GAL4elav-C155, show a significantly shorter lifespan than flies expressing Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 alone.

Coexpression of Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 and debclScer\UAS.T:Ivir\HA1, driven by Scer\GAL4GMR.PF, accelerates the onset of eye neurodegeneration seen in adults expressing Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1. The loss of retinal structure and pigment defects are strongly enhanced by debclScer\UAS.T:Ivir\HA1 expression.

Eyes coexpressing debclScer\UAS.T:Ivir\HA1, BuffyScer\UAS.cQa, and Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1 (all driven by Scer\GAL4GMR.PF) show a significantly weaker degenerative eye phenotype than eyes expressing BuffyScer\UAS.cQa and Hsap\MJDQ78.Scer\UAS.T:Ivir\HA1.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

dsRNA has been transfected into embryos to study the phenotypic consequences of dsRNA interference (RNAi) of the debcl gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
DebcldsRNA.Scer\UAS
DebcldsRNA.UAS
debcldsRNA.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)