Allele Dmel\mod(mdg4)^Z3-5578

General Information
Symbol	Dmel\mod(mdg4)^Z3-5578	Species	D. melanogaster
Name		FlyBase ID	FBal0194707
Feature type	allele	Associated gene	Dmel\mod(mdg4)
Also Known As	mnm^Z3-5578

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Thomas et al., 2005)
Mutagen	ethyl methanesulfonate (Thomas et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The stop mutation is in the C-terminal coding exon, which is present in most mod(mdg4) isoforms. (Thomas et al., 2005) Amino acid replacement: W449@. (Thomas et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class
	female fertile (with Df(3R)GC14) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective (Thomas et al., 2005) meiotic cell cycle defective \| male (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with Df(3R)GC14) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^142Δ10) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^142Δ33) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^B2) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^eGp4) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^T16) (Soltani-Bejnood et al., 2007) meiotic cell cycle defective \| male (with mod(mdg4)^Z3-3298) (Soltani-Bejnood et al., 2007) viable (Soltani-Bejnood et al., 2007)
Phenotype Manifest In
	G2 phase & nuclear chromosome (Thomas et al., 2005) meiosis I & nuclear chromosome (Thomas et al., 2005)
Detailed Description
	Statement Reference Homozygous males show a high frequency of X-Y chromosome nondisjunction in meiosis (51.2%). mod(mdg4)^Z3-5578/Df(3R)GC14 males show a high frequency of 4th chromosome loss (28.1%) and chromosome 2 nondisjunction (43.3%) in meiosis. mod(mdg4)^Z3-5578/Df(3R)GC14 females do not show increased X chromosome nondisjunction compared to controls and the frequency of recombination in large intervals on the X and third chromosomes in the mutant females is normal. (Soltani-Bejnood et al., 2007) mod(mdg4)^Z3-5578 mutants show elevated frequencies of autosomal and sex chromosome nondisjunction. Analysis of mutant spermatocytes shows that nondisjunction is limited largely to meiosis I. Premeiotic and meiotic homolog pairing are normal. The earliest observable phenotype is that of "territory expansion" in late G2, with homologous chromosomes being at a greater distance from one another than in wild-type spermatocytes. mod(mdg4)^Z3-5578 spermatocytes show incomplete chromatin condensation and form up to eight large clumps of chromatin form by metaphase I, instead of the one central mass observed in wild type. The clumps represent bivalents and univalents, consistent with a defect in formation or maintenance of bivalents. In early anaphase I, chromosomes are distributed unevenly and asymmetrically along the spindle. In late anaphase I, there is a high frequency of laggards and polar chromatin masses of unequal size, indicating high frequency of nondisjunction. (Thomas et al., 2005)
External Data
Linkouts
Interactions
Phenotypic Class
NOT Enhancer of
Statement Reference mod(mdg4)^Z3-5578/mod(mdg4)^T16 is a non-enhancer of body color defective phenotype of y² (Soltani-Bejnood et al., 2007)
NOT Suppressor of
Statement Reference mod(mdg4)^Z3-5578/mod(mdg4)^T16 is a non-suppressor of body color defective phenotype of y² (Soltani-Bejnood et al., 2007)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Body colour: the y² phenotype (black bristles, yellow body and wings) is not altered by mod(mdg4)^Z3-5578/mod(mdg4)^T16. (Soltani-Bejnood et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Complements	mod(mdg4)^142Δ49 (Soltani-Bejnood et al., 2007) mod(mdg4)^T6 (Soltani-Bejnood et al., 2007)
Partially complements	mod(mdg4)^142Δ10 (Soltani-Bejnood et al., 2007) mod(mdg4)^142Δ33 (Soltani-Bejnood et al., 2007) mod(mdg4)^B2 (Soltani-Bejnood et al., 2007) mod(mdg4)^eGp4 (Soltani-Bejnood et al., 2007) mod(mdg4)^T16 (Soltani-Bejnood et al., 2007) mod(mdg4)^Z3-3401 (Soltani-Bejnood et al., 2007)
Fails to complement	mod(mdg4)^Z3-3298 (Soltani-Bejnood et al., 2007)
Rescued by	mod(mdg4)^Z3-5578 is rescued by mod(mdg4)^{MNM.hs.T:Avic\GFP-EGFP} (Thomas et al., 2005)
Comments	mod(mdg4)^Z3-5578 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, but they fail to complement mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing a high level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^T16 (44.3%), mod(mdg4)^142Δ10 (46%), mod(mdg4)^142Δ33 (37.3%), mod(mdg4)^eGp4 (50.3%) and mod(mdg4)^B2 (37.3%). mod(mdg4)^Z3-5578 fully complements the lethal/semi-lethal phenotypes of the following mod(mdg4) alleles, and they partially or fully complement mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis, with the transheterozygotes showing only a low level of X-Y nondisjunction (frequency is indicated in parentheses after each allele); mod(mdg4)^142Δ15 (0.56%), mod(mdg4)^142Δ32 (0.95%), mod(mdg4)^142Δ29 (1.54%), mod(mdg4)^142Δ49 (0%), mod(mdg4)^neo129 (4.46%), mod(mdg4)⁰² (1.72%), mod(mdg4)⁰³ (0.07%), mod(mdg4)²⁰ (2.96%), mod(mdg4)¹¹⁷ (0.75%), mod(mdg4)²⁶⁹ (1.44%), mod(mdg4)³²⁴ (2.84%) and mod(mdg4)³⁴⁰ (1.89%). mod(mdg4)^Z3-3401 strongly (but not completely) complements mod(mdg4)^Z3-5578; transheterozygotes show only a low level (1.42%) of X-Y nondisjunction in male meiosis. mod(mdg4)^T6 fully complements mod(mdg4)^Z3-5578 with respect to X-Y chromosome nondisjunction in male meiosis. mod(mdg4)^Z3-5578 fails to complement mod(mdg4)^Z3-3298 with respect to X-Y chromosome nondisjunction in male meiosis; transheterozygotes show 45.2% X-Y nondisjunction. (Soltani-Bejnood et al., 2007)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	mnm^Z3-5578 (Thomas et al., 2005, Thomas and McKee, 2007) mod(mdg4)^Z3-5578 Z3-5578 (Soltani-Bejnood et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Soltani-Bejnood et al., 2007, Genetics 176(1): 161--180 Role of the mod(mdg4) common region in homolog segregation in Drosophila male meiosis. [FBrf0201774] Thomas and McKee, 2007, Genetics 177(2): 785--799 Meiotic pairing and disjunction of mini-X chromosomes in drosophila is mediated by 240-bp rDNA repeats and the homolog conjunction proteins SNM and MNM. [FBrf0205301] Thomas et al., 2005, Cell 123(4): 555--568 Identification of two proteins required for conjunction and regular segregation of achiasmate homologs in Drosophila male Meiosis. [FBrf0189927]

Allele Dmel\mod(mdg4)Z3-5578

Allele Dmel\mod(mdg4)^Z3-5578