FlyBase Allele Report: Dmel\wls[2]

General Information

Symbol

Dmel\wls²

Species

D. melanogaster

Name

FlyBase ID

FBal0194735

Feature type

allele

Associated gene

Dmel\wls

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Banziger et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: P250S.

(Banziger et al., 2006)

The P250S mutation is within the first 'TM' domain.

(Banziger et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:11,168,698..11,168,698 [-]

Nucleotide change:

C11168698T

Reported nucleotide change:

C?T

Amino acid change:

P250S | wls-PA; P250S | wls-PB

Reported amino acid change:

P250S

(Banziger et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with wls¹), with Scer\GAL4^wg-ND382, wls^UAS.Tag:HA

(Banziger et al., 2006)

female sterile (with wls¹), with Scer\GAL4^wg-S180, wls^UAS.Tag:HA

(Banziger et al., 2006)

lethal - all die before end of P-stage (with wls¹)

(Banziger et al., 2006)

male sterile (with wls¹), with Scer\GAL4^wg-ND382, wls^UAS.Tag:HA

(Banziger et al., 2006)

male sterile (with wls¹), with Scer\GAL4^wg-S180, wls^UAS.Tag:HA

(Banziger et al., 2006)

segment polarity phenotype | maternal effect

(Banziger et al., 2006)

some die during pharate adult stage (with wls¹)

(Banziger et al., 2006)

visible | homeotic (with wls¹)

(Banziger et al., 2006)

Phenotype Manifest In

adult antennal segment (with wls¹)

(Banziger et al., 2006)

arista (with wls¹)

(Banziger et al., 2006)

larva | maternal effect

(Banziger et al., 2006)

leg (with wls¹)

(Banziger et al., 2006)

mesonotum (with wls¹)

(Banziger et al., 2006)

ovary (with wls¹), with Scer\GAL4^wg-ND382, wls^UAS.Tag:HA

(Banziger et al., 2006)

ovary (with wls¹), with Scer\GAL4^wg-S180, wls^UAS.Tag:HA

(Banziger et al., 2006)

testis (with wls¹), with Scer\GAL4^wg-ND382, wls^UAS.Tag:HA

(Banziger et al., 2006)

testis (with wls¹), with Scer\GAL4^wg-S180, wls^UAS.Tag:HA

(Banziger et al., 2006)

ventral denticle belt | maternal effect | increased number

(Banziger et al., 2006)

wing (with wls¹)

(Banziger et al., 2006)

wing margin | somatic clone (with wls¹)

(Banziger et al., 2006)

Detailed Description

Statement

Reference

srt¹/srt² pharate adults (the stage at which these mutants die) exhibit rudimentary legs, lack arista and antennal segments and show wing-to-notum transformations.

srt¹/srt² mutant clones cause nicks at the margin of the wing blade.

Larvae produced from srt² germline clones fertilized with srt² mutant sperm display a segment polarity phenotype in which larvae have supernumerary ventral denticles in regions of the epidermis that are naked in wild-type larvae.

srt¹/srt² flies rescued by expression of srt^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^wg-ND382 or Scer\GAL4^wg-S180 exhibit testicular and ovarian phenotypes as well as male and female sterility.

(Banziger et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

wls²/wls² is a suppressor of ommatidium phenotype of wg^hs.2sev

(Banziger et al., 2006)

wls²/wls² is a suppressor of interommatidial bristle phenotype of wg^hs.2sev

(Banziger et al., 2006)

wls²/wls² is a suppressor of eye phenotype of wg^hs.2sev

(Banziger et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Mosaic animals in which most cells in the eye have a srt² genotype show a significant reversion of the rough eye phenotype seen when following expression of the wg^hs.2sev transgene.

(Banziger et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

wls¹

(Banziger et al., 2006)

Rescued by

wls² is rescued by wls^αTub84B.PB

(Banziger et al., 2006)

Partially rescued by

wls²/wls¹ is partially rescued by wls^UAS.Tag:HA/Scer\GAL4^wg-ND382

(Banziger et al., 2006)

wls²/wls¹ is partially rescued by wls^UAS.Tag:HA/Scer\GAL4^wg-S180

(Banziger et al., 2006)

Comments

Expression of srt^{Scer\UAS.T:Ivir\HA1}, under the control of either Scer\GAL4^wg-ND382 or Scer\GAL4^wg-S180, rescues >80% of srt¹/srt² mutants to be viable adults.

(Banziger et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a repressor of the rough eye phenotype caused by expression of the wg^hs.2sev transgene.

(Banziger et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

srt²

wls²

(Banziger et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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