Allele Dmel\ik2^DN.Scer\UAS

General Information
Symbol	Dmel\ik2^DN.Scer\UAS	Species	D. melanogaster
Name		FlyBase ID	FBal0194831
Feature type	allele	Associated gene	Dmel\ik2

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (Oshima, 2006) in vitro construct - site directed mutagenesis (Oshima, 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	ik2^Scer\UAS.cOa (Oshima, 2006)
Nature of the lesion	Statement Reference The K41A mutation is within the catalytic center of the kinase domain. (Oshima, 2006) Amino acid replacement: K41A. (Oshima, 2006)
Carried in construct	P{UAS-ik2.DN} (Oshima et al., 2006, Lee et al., 2009, Bitan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective, with Scer\GAL4^Act5C.PI (Oshima et al., 2006) neuroanatomy defective \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009)
Phenotype Manifest In
	arista lateral, with Scer\GAL4^ap-md544 (Oshima et al., 2006) dendrite & dorsal multidendritic neuron ddaC \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009) macrochaeta, with Scer\GAL4^ap-md544 (Oshima et al., 2006) macrochaeta, with Scer\GAL4^{neur-GAL4-A101} (Bitan et al., 2010) microtubule, with Scer\GAL4^{neur-GAL4-A101} (Bitan et al., 2010) S2 cell-line & filamentous actin, with Scer\GAL4^Act5C.PI (Oshima et al., 2006) scutellar bristle, with Scer\GAL4^{neur-GAL4-A101} (Bitan et al., 2010)
Detailed Description
	Statement Reference Expression of ik2[DN.Scer\UAS] under the control of Scer\GAL4[neur-GAL4-A101] results in shorter and thicker anterior and posterior scutellar macrochaetae. Approximately 23% of these mutant bristles exhibit wild-type morphology, in which the shaft diameter is the widest at the bristle base and tapered toward the tip. In most of the mutant fly bristles, the widest shaft diameter is not found at the base of the bristle. Approximately 75% of the mutant bristles exhibit an altered growth direction that is not axially biased and 51% terminate in several minitips instead of a single tip. The middle third of the mutant bristles show moderately disorganized ridges that are shallower and more numerous than the wild-type bristle surface, which is characterized by straight ridges and valleys, suggesting that alterations in actin organization occurs in the mutants during the middle phase of bristle elongation. Microtubule organisation and polarization is affected in bristles expressing ik2[DN.Scer\UAS] under the control of Scer\GAL4[neur-GAL4-A101]. (Bitan et al., 2010) The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2[DN.Scer\UAS] under the control of Scer\GAL4[ppk.PG], indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals). (Lee et al., 2009) Expression of ik2^DN.Scer\UAS in S2 cells, under the control of Scer\GAL4^Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology. In the cells with the serrate morphology, there is a slight deceleration of the retrograde F actin flow. Flies that express ik2^DN.Scer\UAS under the control of Scer\GAL4^ap-md544, show gnarled, forked macrochaetae. These flies also show abnormal aristae; the unicellular laterals are split at their tips or branched. (Oshima et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^ap-md544, ik2^DN.Scer\UAS has arista lateral phenotype, enhanceable by Ark^{dsRNA.Scer\UAS}, Scer\GAL4^ap-md544 (Oshima et al., 2006) Scer\GAL4^ap-md544, ik2^DN.Scer\UAS has arista lateral phenotype, enhanceable by Nc^{dsRNA.cOa.Scer\UAS}, Scer\GAL4^ap-md544 (Oshima et al., 2006) Scer\GAL4^ap-md544, ik2^DN.Scer\UAS has arista lateral phenotype, enhanceable by th^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^ap-md544 (Oshima et al., 2006)
Suppressed by
Statement Reference Scer\GAL4^ap-md544, ik2^DN.Scer\UAS has arista lateral phenotype, suppressible by Scer\GAL4^ap-md544/th^{dsRNA.cOa.Scer\UAS} (Oshima et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The excess branching of arista seen in flies that express ik2^DN.Scer\UAS under the control of Scer\GAL4^ap-md544 is suppressed by th^{dsRNA.cOa.Scer\UAS} and enhanced by th^{Scer\UAS.T:Hsap\MYC}. The Scer\GAL4^ap-md544>ik2^DN.Scer\UAS arista lateral phenotype is enhanced by RNAi of either Ark or Nc, generated by expression of Ark^{dsRNA.Scer\UAS} or Nc^{dsRNA.cOa.Scer\UAS}. Coexpression of th^{Scer\UAS.T:Hsap\MYC} and ik2^DN.Scer\UAS under the control of Scer\GAL4^Act5C.PI has an additive on S2 cell morphology. (Oshima et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in a plasmid and transfected into S2 cells to study the effect of the expressed product on cell shape and F actin dynamics. (Oshima et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ik2^DN.Scer\UAS
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Bitan et al., 2010, Mol. Cell. Biol. 30(2): 496--507 Asymmetric microtubule function is an essential requirement for polarized organization of the Drosophila bristle. [FBrf0209571] Lee et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(15): 6363--6368 Drosophila IKK-related kinase Ik2 and Katanin p60-like 1 regulate dendrite pruning of sensory neuron during metamorphosis. [FBrf0207681] Oshima et al., 2006, Curr. Biol. 16(15): 1531--1537 IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis. [FBrf0193419]
Supplementary material	Oshima, 2006, Curr. Biol. 16(15): [FBrf0199092]

Allele Dmel\ik2DN.Scer\UAS

Allele Dmel\ik2^DN.Scer\UAS