|Feature type||allele||Associated gene||Dmel\ik2|
|Mutagen||in vitro construct - site directed mutagenesis, in vitro construct - amino acid replacement|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
The K41A mutation is within the catalytic center of the kinase domain.
Amino acid replacement: K41A.
|Carried in construct|
|Phenotype Manifest In|
Expression of ik2[DN.Scer\UAS] under the control of Scer\GAL4[neur-GAL4-A101] results in shorter and thicker anterior and posterior scutellar macrochaetae. Approximately 23% of these mutant bristles exhibit wild-type morphology, in which the shaft diameter is the widest at the bristle base and tapered toward the tip. In most of the mutant fly bristles, the widest shaft diameter is not found at the base of the bristle. Approximately 75% of the mutant bristles exhibit an altered growth direction that is not axially biased and 51% terminate in several minitips instead of a single tip. The middle third of the mutant bristles show moderately disorganized ridges that are shallower and more numerous than the wild-type bristle surface, which is characterized by straight ridges and valleys, suggesting that alterations in actin organization occurs in the mutants during the middle phase of bristle elongation. Microtubule organisation and polarization is affected in bristles expressing ik2[DN.Scer\UAS] under the control of Scer\GAL4[neur-GAL4-A101].
The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2[DN.Scer\UAS] under the control of Scer\GAL4[ppk.PG], indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).
Expression of ik2DN.Scer\UAS in S2 cells, under the control of Scer\GAL4Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology. In the cells with the serrate morphology, there is a slight deceleration of the retrograde F actin flow. Flies that express ik2DN.Scer\UAS under the control of Scer\GAL4ap-md544, show gnarled, forked macrochaetae. These flies also show abnormal aristae; the unicellular laterals are split at their tips or branched.
|Phenotype Manifest In|
Scer\GAL4ap-md544, ik2DN.Scer\UAS has arista lateral phenotype, enhanceable by ArkdsRNA.Scer\UAS, Scer\GAL4ap-md544
Scer\GAL4ap-md544, ik2DN.Scer\UAS has arista lateral phenotype, enhanceable by NcdsRNA.cOa.Scer\UAS, Scer\GAL4ap-md544
The excess branching of arista seen in flies that express ik2DN.Scer\UAS under the control of Scer\GAL4ap-md544 is suppressed by thdsRNA.cOa.Scer\UAS and enhanced by thScer\UAS.T:Hsap\MYC. The Scer\GAL4ap-md544>ik2DN.Scer\UAS arista lateral phenotype is enhanced by RNAi of either Ark or Nc, generated by expression of ArkdsRNA.Scer\UAS or NcdsRNA.cOa.Scer\UAS. Coexpression of thScer\UAS.T:Hsap\MYC and ik2DN.Scer\UAS under the control of Scer\GAL4Act5C.PI has an additive on S2 cell morphology.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
Carried in a plasmid and transfected into S2 cells to study the effect of the expressed product on cell shape and F actin dynamics.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 4 )|