The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2dsRNA.Scer\UAS under the control of Scer\GAL4ppk.PG, indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals).
The microtubules remain intact in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing ik2dsRNA.Scer\UAS under the control of Scer\GAL4ppk.PG (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage).
Expression of ik2dsRNA.Scer\UAS in S2 cells, under the control of Scer\GAL4Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology.
Embryos treated with ik2 RNAi, generated by expression of ik2dsRNA.Scer\UAS under the control of Scer\GAL4btl.PS, show tracheal terminal branching defects. These include the duplication of terminal cells and the misorientation of the actin core.
Flies treated with ik2 RNAi, generated by expression of ik2dsRNA.Scer\UAS under the control of Scer\GAL4ap-md544, show gnarled, forked macrochaetae.
Carried in a plasmid and transfected into S2 cells. Transcription from the plasmid should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene.
Carried in plasmid "pUAST-DmIKKε IR" and transfected into S2 cells. Transcription from the P{UAS-ik2.IR} construct should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene.