Allele Dmel\ik2^{dsRNA.Scer\UAS}

General Information
Symbol	Dmel\ik2^{dsRNA.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0194832
Feature type	allele	Associated gene	Dmel\ik2

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Oshima, 2006, Kuranaga et al., 2006) in vitro construct - RNAi (Oshima, 2006, Kuranaga et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotides 1-500 of the ik2 cDNA has been inserted as an inverted repeat and driven by Scer\UAS sequences. (Kuranaga et al., 2006) Scer\UAS regulatory sequences drive expression of an inverted repeat of a ik2 fragment. (Oshima, 2006)
Carried in construct	P{UAS-ik2.dsRNA} (Oshima et al., 2006, Kuranaga et al., 2006, Lee et al., 2009, Koto et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	cell shape defective, with Scer\GAL4^Act5C.PI (Oshima et al., 2006) neuroanatomy defective \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009)
Phenotype Manifest In
	dendrite & dorsal multidendritic neuron ddaC \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009) macrochaeta, with Scer\GAL4^ap-md544 (Oshima et al., 2006) macrochaeta, with Scer\GAL4^sca-P309 (Koto et al., 2009) microtubule & dorsal multidendritic neuron ddaC \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009) terminal tracheal cell, with Scer\GAL4^btl.PS (Oshima et al., 2006)
Detailed Description
	Statement Reference Bristles are shorter and thicker in the adult notum of Scer\GAL4[sca-P309]; ik2[dsRNA.Scer\UAS] flies. (Koto et al., 2009) The proximal dendrites remain intact for most ddaC neurons at 18 hours after puparium formation (APF) in animals expressing ik2[dsRNA.Scer\UAS] under the control of Scer\GAL4[ppk.PG], indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals). The microtubules remain intact in the dendrites of ddaC neurons at 6 hours after puparium formation (APF) in animals expressing ik2[dsRNA.Scer\UAS] under the control of Scer\GAL4[ppk.PG] (microtubules in the dendrites of wild-type ddaC neurons show breakage at this stage). (Lee et al., 2009) Expression of ik2^{dsRNA.Scer\UAS} in S2 cells, under the control of Scer\GAL4^Act5C.PI, does not affect cell viability. However, these S2 cells show an increased likelihood to have a serrate or stellate morphology, instead of the typical round morphology. Embryos treated with ik2 RNAi, generated by expression of ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^btl.PS, show tracheal terminal branching defects. These include the duplication of terminal cells and the misorientation of the actin core. Flies treated with ik2 RNAi, generated by expression of ik2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ap-md544, show gnarled, forked macrochaetae. (Oshima et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^sca-P309, ik2^{dsRNA.Scer\UAS} has macrochaeta phenotype, enhanceable by Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}, Scer\GAL4^sca-P309 (Koto et al., 2009) Scer\GAL4^sca-P309, ik2^{dsRNA.Scer\UAS} has macrochaeta phenotype, enhanceable by th^{Scer\UAS.T:Zzzz\FLAG}/Scer\GAL4^sca-P309 (Koto et al., 2009)
Other
Statement Reference BacA\p35^Scer\UAS.cHa, Scer\GAL4^sca-P309, ik2^{dsRNA.Scer\UAS} has macrochaeta phenotype (Koto et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Overexpression of th[Scer\UAS.T:Zzzz\FLAG] enhances the short-bristle phenotype of Scer\GAL4[sca-P309]; ik2[dsRNA.Scer\UAS] flies. Co-expression of Nc[C318G.Scer\UAS.T:Avic\GFP-EGFP] in Scer\GAL4[sca-P309]; ik2[dsRNA.Scer\UAS] flies significantly enhances the short-bristle phenotype. (Koto et al., 2009)
Xenogenetic Interactions
Statement Reference Co-expression of BacA\p35[Scer\UAS.cHa] and ik2[dsRNA.Scer\UAS], both under the control of Scer\GAL4[sca-P309] generates a short bristle phenotype, as found in Scer\GAL4[sca-P309]; ik2[dsRNA.Scer\UAS] mutants. (Koto et al., 2009)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Carried in plasmid "pUAST-DmIKKε IR" and transfected into S2 cells. Transcription from the P{UAS-ik2.IR} construct should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene. (Kuranaga et al., 2006) Carried in a plasmid and transfected into S2 cells. Transcription from the plasmid should produce ik2 dsRNA, resulting in dsRNA interference (RNAi) of the ik2 gene. (Oshima et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	ik2^{dsRNA.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Koto et al., 2009, J. Cell Biol. 187(2): 219--231 Temporal regulation of Drosophila IAP1 determines caspase functions in sensory organ development. [FBrf0209002] Lee et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(15): 6363--6368 Drosophila IKK-related kinase Ik2 and Katanin p60-like 1 regulate dendrite pruning of sensory neuron during metamorphosis. [FBrf0207681] Kuranaga et al., 2006, Cell 126(3): 583--596 Drosophila IKK-related kinase regulates nonapoptotic function of caspases, via degradation of IAPs. [FBrf0194448] Oshima et al., 2006, Curr. Biol. 16(15): 1531--1537 IKK epsilon regulates F actin assembly and interacts with Drosophila IAP1 in cellular morphogenesis. [FBrf0193419]
Supplementary material	Oshima, 2006, Curr. Biol. 16(15): [FBrf0199092]

Allele Dmel\ik2dsRNA.Scer\UAS

Allele Dmel\ik2^{dsRNA.Scer\UAS}