FlyBase Allele Report: Dmel\Myo61F[UAS.cHa]

General Information

Symbol

Dmel\Myo61F^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hozumi

FlyBase ID

FBal0195155

Feature type

allele

Associated gene

Dmel\Myo61F

Associated Insertion(s)

Carried in Construct

P{UAS-Myo61F.H}

Also Known As

UAS-Myo61F

Key Links

Transgenic product class

cDNA

(Hozumi et al., 2006)

wild_type

(Hozumi et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Hozumi et al., 2006)

wild_type

(Hozumi et al., 2006)

Mutagen

in vitro construct

(Hozumi et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Myo61F.H}

Cytology

Description

UASt regulatory sequences drive expression of the complete Myo61F open reading frame (from cDNA GH04201).

(Hozumi et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Myo61F

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 fail to undergo the male genitalia rotation seen in wild type.

(Okumura et al., 2015)

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^tsh-md621 results in sinistral male genitalia rotation (instead of the wild-type dextral rotation).

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^Abd-B-LDN results in no rotation of the male genitalia in 91% of cases and sinistral rotation of the male genitalia in 9% of cases.

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^{Myo31DF-NP1548} results in full sinistral rotation of the male genitalia in 100% of cases.

(Petzoldt et al., 2012)

Overexpression of Myo61F^Scer\UAS.cHa in the whole hindgut, under the control of Scer\GAL4^byn-Gal4 results in 100% left-right symmetry inversion in the midgut and hindgut simultaneously. In contrast, the foregut of Myo61F^Scer\UAS.cHa mutant embryos show normal handedness, indicating that these left-right defects are heterotaxic.

(Taniguchi et al., 2007)

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^byn-Gal4 results in inversion of the normal left-right asymmetry of the embryonic midgut and hindgut in 100% of embryos.

(Hozumi et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^da.PU/Myo61F^UAS.cHa is an enhancer of abnormal axis specification phenotype of Myo31DF^L152

(Okumura et al., 2015)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Myo61F^UAS.cHa is a non-suppressor of embryonic/larval posterior midgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Scer\GAL4^da.PU/Myo61F^UAS.cHa is a non-suppressor of embryonic/larval hindgut phenotype of Myo31DF^L152

(Okumura et al., 2015)

Scer\GAL4^ptc-559.1/Myo61F^UAS.cHa is a non-suppressor of male genitalia phenotype of Myo31DF^K2

(Okumura et al., 2015)

Other

Statement

Reference

Myo31DF^K2, Myo61F^UAS.cHa, Scer\GAL4^ptc-559.1 has male genitalia phenotype

(Okumura et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^da.PU enhances the frequency of left/right (l/r) inversion in the posterior midgut and the hindgut seen in homozygous Myo31DF^L152 mutant embryos from 80% to 100%.

Expression of Myo61F^Scer\UAS.cHa under the control of Scer\GAL4^ptc-559.1 does not suppress the change in direction of male genitalia rotation seen in Myo31DF^K2/Myo31DF^L152 mutant pupae. Mutants expressing Myo61F^Scer\UAS.cHa continue to exhibit the left-handed (sinistral) rotation seen in Myo31DF^K2/Myo31DF^L152 mutations, as opposed to the dextral (right-handed) rotation seen in wild type. Expressing Myo61F^Scer\UAS.cHa in Myo31DF^K2 heterozygotes produces partial sinistral phenotypes.

(Okumura et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Myo61F^Scer\UAS.cHa

Myo61F^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hozumi

Secondary FlyBase IDs

References (6)

Report Sections

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