|Feature type||allele||Associated gene||Dmel\cyc|
|Also Known As||cyc0|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: K159@.
Has a nonsense mutation in the PAS-encoding region.
Nucleotide substitution: A586T. Amino acid replacement: K159@.
|Phenotype Manifest In|
1 day memory retention is normal after spaced training in an olfactory conditioning assay.
Naive and trained responses to a magnetic field are not impaired in cyc mutant flies.
cyc mutants do not display phenotypes related to either survival time under starvation conditions or glycogen storage. Total food consumption is also unchanged in these mutants compared to controls.
The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc.
cyc01 has no effect on experience-dependent responses in sleep need.
With 5-HTP treatment, daily sleep and night-time sleep bout length are increase in cyc01 mutant flies, compared to controls where daily sleep is increased but there is no change in night-time sleep bout length.
cyc mutant larvae are highly sensitive to light (shown in light avoidance assays), even at low intensities (150 lux). cyc larvae entrained in LD cycles for 2-3 days and then shifted into DD, that are tested for their ability to avoid light at 3hr intervals on the second day of DD do not show a detectable rhythm, appearing constitutively sensitive.
There is no significant increase in the time spent copulating by pairs of cyc01 homozygous flies compared to wild-type pairs.
Long term memory of courtship conditioning (reduction in time spent in courtship behaviour 5 days after a 7 hour conditioning) is normal in cyc0 homozygous males.
The diurnal changes in the mean electroantennogram (EAG) response to ethyl acetate which are seen in wild-type flies maintained in 12 hour light:12 hour dark cycles are abolished in cyc01 flies.
cyc01 mutant females mated to cyc01 mutant males lay significantly less eggs and produce significantly less progeny than wild-type flies. The percentage of unfertilised eggs from this mating is not significantly greater than for wild-type.
Mutant females show a disproportionately large sleep rebound after sleep deprivation (in constant darkness). Increasing the length of sleep deprivation to more than 3 hours results in the flies showing large increases in sleep that persists as long as the flies are recorded (up to 16 days). A second period of sleep deprivation results in further increases in sleep. The flies begin to die if deprived of sleep for more than 10 hours (mortality is 33 +/- 4% for 12 hours of sleep deprivation). Mutant flies that are deprived of sleep for 30 minutes each hour for 24 hours do not die, indicating that it is not the stimulus used for sleep deprivation that causes the flies to die after 12 hours of constant sleep deprivation. The genetic background and age of the flies does not influence the sleep phenotype. Mutant males recover 100% of lost sleep and die after 12 hours of sleep deprivation. Exposure of mutant females to heat before 12 hours of sleep deprivation protects them from the lethal effects of sleep deprivation and reduces homeostatic drive (sleep rebound). cyc01/Df(3L)kto2 flies also show an exaggerated homeostatic response (sleep rebound) and deaths after 12 hours of sleep deprivation.
About a quarter of cyc0 adult brains exhibit an abnormal dorsal projection from the l-LNv cells. In about 30% of these mutant specimens the projections are asymmetric within a single individual: one hemisphere might contain a bundle of dorsally projected axons; but in the contralateral hemisphere, only one or two axons might project into the dorsal brain. In other cyc0 adults, axons stemming from the region project irregularly into a median brain region.
Homozygotes are arrhythmic with respect to locomotor activity and adult eclosion under constant darkness conditions. This phenotype is not altered if the flies are also mutant for either perL1 or pers. Only 64% of homozygotes have discernible locomotor rhythms under 12 hour light:12 hour dark conditions. Heterozygotes have robust locomotor rhythms with no hint of arrhythmicity, but they have altered periods with rhythms 1 hour longer than normal. Homozygotes behave normally in an optomotor behaviour test.
|Phenotype Manifest In|
Expression of Gprk2[Scer\UAS.cTa] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22] induces a robust electroantennogram response in a cyc background.
Expression of Clk[DN.Scer\UAS] in the fat body, under the control of Scer\GAL4[to.PD] in a cyc background fails to affect starvation sensitivity. Glycogen levesl are lower than those of cyc flies, but the decrease is less than that seen in heterozygous cyc flies with a disrupted fat body clock.
|Complementation & Rescue Data|
|Fails to complement|
|Partially rescued by|
|Not rescued by|
The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc. This phenotype is not rescued by cyc[Scer\UAS.T:Hsap\MYC]; Scer\GAL4[Or83b.2.642.T:Hsim\VP22].
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 4 )|
|Secondary FlyBase IDs|
|References ( 48 )|
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|Recent research papers ( 4 )|
|Recent reviews (0)|
|All reviews listed in FlyBase were published before 2011|