Allele Dmel\cyc⁰¹

General Information
Symbol	Dmel\cyc⁰¹	Species	D. melanogaster
Name		FlyBase ID	FBal0195440
Feature type	allele	Associated gene	Dmel\cyc
Also Known As	cyc⁰

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Rutila et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:19,807,982..19,807,982 evidence=experimental na_change=A19807982T pr_change=K159@\|cyc-PA reported_na_change=A586T reported_pr_change=K159@ (Rutila et al., 1998, Park et al., 2000)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: K159@. (Rosbash, 2006.8.10) Has a nonsense mutation in the PAS-encoding region. (Park et al., 2000) Nucleotide substitution: A586T. Amino acid replacement: K159@. (Rutila et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective (Yuan et al., 2006, Shaw et al., 2002) behavior defective (with Df(3L)kto2) (Shaw et al., 2002) circadian rhythm defective (Tanoue et al., 2004) eclosion rhythm defective \| recessive (Rutila et al., 1998) locomotor rhythm defective (Peng et al., 2003) locomotor rhythm defective \| recessive (Park et al., 2000, Rutila et al., 1998) mating rhythm defective \| male limited \| recessive (Fujii et al., 2007) visual behavior defective (Mazzoni et al., 2005)
Phenotype Manifest In
	LN period neuron (Park et al., 2000)
Detailed Description
	Statement Reference 1 day memory retention is normal after spaced training in an olfactory conditioning assay. (Chen et al., 2012) Triglyceride levels in cyc[01] mutants are similar to wild-type. (Diangelo et al., 2011) Naive and trained responses to a magnetic field are not impaired in cyc[01] mutant flies. (Gegear et al., 2010) cyc[01] mutants do not display phenotypes related to either survival time under starvation conditions or glycogen storage. Total food consumption is also unchanged in these mutants compared to controls. (Xu et al., 2008) The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc[01]. (Fujii et al., 2007) cyc⁰¹ has no effect on experience-dependent responses in sleep need. (Ganguly-Fitzgerald et al., 2006) With 5-HTP treatment, daily sleep and night-time sleep bout length are increase in cyc⁰¹ mutant flies, compared to controls where daily sleep is increased but there is no change in night-time sleep bout length. (Yuan et al., 2006) cyc[01] mutant larvae are highly sensitive to light (shown in light avoidance assays), even at low intensities (150 lux). cyc[01] larvae entrained in LD cycles for 2-3 days and then shifted into DD, that are tested for their ability to avoid light at 3hr intervals on the second day of DD do not show a detectable rhythm, appearing constitutively sensitive. (Mazzoni et al., 2005) There is no significant increase in the time spent copulating by pairs of cyc⁰¹ homozygous flies compared to wild-type pairs. (Beaver and Giebultowicz, 2004) Long term memory of courtship conditioning (reduction in time spent in courtship behaviour 5 days after a 7 hour conditioning) is normal in cyc⁰ homozygous males. (Sakai et al., 2004) The diurnal changes in the mean electroantennogram (EAG) response to ethyl acetate which are seen in wild-type flies maintained in 12 hour light:12 hour dark cycles are abolished in cyc⁰¹ flies. (Tanoue et al., 2004) Circadian locomotor rhythms are also absent in cyc⁰¹/cyc⁰¹ flies. (Peng et al., 2003) cyc⁰¹ mutant females mated to cyc⁰¹ mutant males lay significantly less eggs and produce significantly less progeny than wild-type flies. The percentage of unfertilised eggs from this mating is not significantly greater than for wild-type. (Beaver et al., 2002) Mutant females show a disproportionately large sleep rebound after sleep deprivation (in constant darkness). Increasing the length of sleep deprivation to more than 3 hours results in the flies showing large increases in sleep that persists as long as the flies are recorded (up to 16 days). A second period of sleep deprivation results in further increases in sleep. The flies begin to die if deprived of sleep for more than 10 hours (mortality is 33 +/- 4% for 12 hours of sleep deprivation). Mutant flies that are deprived of sleep for 30 minutes each hour for 24 hours do not die, indicating that it is not the stimulus used for sleep deprivation that causes the flies to die after 12 hours of constant sleep deprivation. The genetic background and age of the flies does not influence the sleep phenotype. Mutant males recover 100% of lost sleep and die after 12 hours of sleep deprivation. Exposure of mutant females to heat before 12 hours of sleep deprivation protects them from the lethal effects of sleep deprivation and reduces homeostatic drive (sleep rebound). cyc⁰¹/Df(3L)kto2 flies also show an exaggerated homeostatic response (sleep rebound) and deaths after 12 hours of sleep deprivation. (Shaw et al., 2002) About a quarter of cyc⁰ adult brains exhibit an abnormal dorsal projection from the l-LN_v cells. In about 30% of these mutant specimens the projections are asymmetric within a single individual: one hemisphere might contain a bundle of dorsally projected axons; but in the contralateral hemisphere, only one or two axons might project into the dorsal brain. In other cyc⁰ adults, axons stemming from the region project irregularly into a median brain region. (Park et al., 2000) Homozygotes are arrhythmic with respect to locomotor activity and adult eclosion under constant darkness conditions. This phenotype is not altered if the flies are also mutant for either per^L1 or per^s. Only 64% of homozygotes have discernible locomotor rhythms under 12 hour light:12 hour dark conditions. Heterozygotes have robust locomotor rhythms with no hint of arrhythmicity, but they have altered periods with rhythms 1 hour longer than normal. Homozygotes behave normally in an optomotor behaviour test. (Rutila et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference Expression of Gprk2[Scer\UAS.cTa] under the control of Scer\GAL4[Or83b.2.642.T:Hsim\VP22] induces a robust electroantennogram response in a cyc[01] background. (Tanoue et al., 2008) Expression of Clk[DN.Scer\UAS] in the fat body, under the control of Scer\GAL4[to.PD] in a cyc[01] background fails to affect starvation sensitivity. Glycogen levesl are lower than those of cyc[01] flies, but the decrease is less than that seen in heterozygous cyc[01] flies with a disrupted fat body clock. (Xu et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	cyc⁰² (Park et al., 2000)
Rescued by	cyc⁰¹/cyc⁰¹ is rescued by cyc^{7.2.T:Zzzz\FLAG} (Abruzzi et al., 2011) cyc⁰¹/cyc⁰¹ is rescued by Scer\GAL4^Or22a.8197/cyc^{Scer\UAS.T:Hsap\MYC} (Tanoue et al., 2004) cyc⁰¹/cyc⁰¹ is rescued by Scer\GAL4^{Orco.2.642.T:Hsim\VP22}/cyc^{Scer\UAS.T:Hsap\MYC} (Tanoue et al., 2004)
Partially rescued by	cyc⁰¹/cyc⁰¹ is partially rescued by cyc^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^P2.4.Pdf (Peng et al., 2003)
Not rescued by	cyc⁰¹/cyc⁰¹ is not rescued by Scer\GAL4^{Orco.2.642.T:Hsim\VP22}/cyc^{Scer\UAS.T:Hsap\MYC} (Fujii et al., 2007)
Comments	The pronounced circadian rhythm of close-proximity encounters seen in wild-type male:female pairs is lost when the male is homozygous for cyc[01]. This phenotype is not rescued by cyc[Scer\UAS.T:Hsap\MYC]; Scer\GAL4[Or83b.2.642.T:Hsim\VP22]. (Fujii et al., 2007) cyc⁰¹ and cyc⁰² are non complementing both for behaviour and for a severe reduction in levels of per protein. (Park et al., 2000)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	cyc01 (Rosbash, 2006.8.10) cyc⁰ (Yuan et al., 2006, Park et al., 2000, Yuan et al., 2005, Yuan et al., 2005, Rosbash, 2006.8.10, Chen et al., 2012, Mazzoni et al., 2005, Blanchard et al., 2010, Gegear et al., 2010, Ruben et al., 2012) Cyc⁰ (Glossop et al., 1999) cyc⁰¹ (Fujii et al., 2007, Yu et al., 2006, Benito et al., 2008, Ganguly-Fitzgerald et al., 2006, Diangelo et al., 2011, Tanoue et al., 2008, Fujii et al., 2008, Xu et al., 2008, Abruzzi et al., 2011, Bahn et al., 2009)
Name Synonym
Secondary FlyBase IDs
	FBal0090449 FBal0105034
References ( 48 )

Generate a list of	All references relating to this allele ( 48 )

List References by type	Research paper ( 39 ) Supplementary material ( 2 ) Review ( 4 ) Personal communication to FlyBase ( 1 ) Abstract ( 2 )
Recent research papers ( 4 )
	Chen et al., 2012, Science 335(6069): 678--685 Visualizing long-term memory formation in two neurons of the Drosophila brain. [FBrf0217453] Ruben et al., 2012, J. Biol. Rhythms 27(5): 353--364 A mechanism for circadian control of pacemaker neuron excitability. [FBrf0219602] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Diangelo et al., 2011, PLoS ONE 6(5): e19921 The central clock neurons regulate lipid storage in Drosophila. [FBrf0213791] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...

Allele Dmel\cyc01

Allele Dmel\cyc⁰¹