FlyBase Allele Report: Dmel\bmm[UAS.cGa]

General Information

Symbol

Dmel\bmm^UAS.cGa

Species

D. melanogaster

Name

FlyBase ID

FBal0195575

Feature type

allele

Associated gene

Dmel\bmm

Associated Insertion(s)

Carried in Construct

P{UAS-bmm.cGa}

Also Known As

UAS-bmm

Key Links

Transgenic product class

cDNA

(Gronke et al., 2005)

wild_type

(Gronke et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gronke et al., 2005)

wild_type

(Gronke et al., 2005)

Mutagen

in vitro construct

(Gronke et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-bmm.cGa}

Cytology

Description

UASt regulatory sequences drive expression of an embryonic bmm cDNA.

(Gronke et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

bmm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates obesity

modeled by Lsd-1¹

(Beller et al., 2010)

ameliorates Parkinson's disease 1

modeled by CG7900^15.3

(Girard et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult fat body, with Scer\GAL4^fat

(Gronke et al., 2005)

lipid droplet & larval oenocyte, with Scer\GAL4^Lsp2.PH

(Gutierrez et al., 2007)

lipid droplet & larval oenocyte, with Scer\GAL4^ppl.PP

(Gutierrez et al., 2007)

Detailed Description

Statement

Reference

Expression of bmm^Scer\UAS.cGa using Scer\GAL4^ppl.PP increases neutral lipids in oenocytes.

(Tian et al., 2011)

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat decreases the fat content of flies compared to controls.

(Beller et al., 2010)

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^Lsp2.PH rescues the increase in triglyceride levels and the heart defects normally caused by a high-fat diet.

Expression of bmm^Scer\UAS.cGa under the simultaneous control of both Scer\GAL4^Scer\UAS.cHa and Scer\GAL4^tin.cBa does not rescue the increase in triglyceride levels caused by a high-fat diet. However, the heart defects normally caused by a high-fat diet are rescued in these animals.

(Birse et al., 2010)

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^ppl.PP results in an accumulation of lipid droplets in the oenocytes of 92% of fed larvae (this contrasts with wild-type larvae, where oenocytes accumulate lipid droplets specifically under starvation conditions).

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^Lsp2.PH results in an accumulation of lipid droplets in the oenocytes of 100% of fed larvae.

(Gutierrez et al., 2007)

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat in fed flies depletes the storage fat (measured as triacylglycerol content) of immature (less than 1 day old) and mature (6 days old) adults by 96% and 46%, respectively, compared to controls.

Fat body cells of immature adults expressing bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat have storage droplets that are reduced in number and size.

Mature adults expressing bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat show no difference in carbohydrate storage (measured as glycogen content) compared to controls.

(Gronke et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Lsp2.3.1/bmm^UAS.cGa is a suppressor of increased cell death | oogenesis phenotype of Scer\GAL4^Lsp2.3.1, crc^KK111018

(Grmai et al., 2024)

Scer\GAL4^Lsp2.3.1/bmm^UAS.cGa is a suppressor of increased size | adult stage phenotype of Scer\GAL4^Lsp2.3.1, crc^KK111018

(Grmai et al., 2024)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of decreased cell number | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

bmm^UAS.cGa/Scer\GAL4^elav.PLu is a suppressor | partially of abnormal neuroanatomy | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

bmm^UAS.cGa/Scer\GAL4^elav.PLu is a suppressor | partially of decreased cell number | adult stage | progressive phenotype of Tace¹

(Muliyil et al., 2020)

NOT Suppressor of

Statement

Reference

bmm^UAS.cGa/Scer\GAL4^ninaE.PT is a non-suppressor of abnormal neurophysiology | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

bmm^UAS.cGa/Scer\GAL4^ninaE.PT is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^fat, bmm^UAS.cGa has phenotype, suppressible | partially by Lsd-2^UAS.cGa, Scer\GAL4^fat

(Gronke et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^Lsp2.3.1/bmm^UAS.cGa is a suppressor of egg chamber | decreased number phenotype of Scer\GAL4^Lsp2.3.1, crc^KK111018

(Grmai et al., 2024)

Scer\GAL4^Lsp2.3.1/bmm^UAS.cGa is a suppressor of lipid droplet | adult stage phenotype of Scer\GAL4^Lsp2.3.1, crc^KK111018

(Grmai et al., 2024)

Scer\GAL4^Lsp2.3.1/bmm^UAS.cGa is a suppressor of adult fat body phenotype of Scer\GAL4^Lsp2.3.1, crc^KK111018

(Grmai et al., 2024)

bmm^UAS.cGa, Scer\GAL4^ninaE.PT is a suppressor of photoreceptor neuron | adult stage phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^ninaE.PT

(Girard et al., 2021)

bmm^UAS.cGa, Scer\GAL4^ninaE.PT is a suppressor of lipid droplet | increased number | adult stage phenotype of Hsap\SNCA^A53T.UAS, Scer\GAL4^ninaE.PT

(Girard et al., 2021)

bmm^UAS.cGa/Scer\GAL4^elav.PLu is a suppressor | partially of retina phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of pigment cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of eye photoreceptor cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

Scer\GAL4^sv.PJ/bmm^UAS.cGa is a suppressor of retina phenotype of Tace¹

(Muliyil et al., 2020)

bmm^UAS.cGa/Scer\GAL4^elav.PLu is a suppressor | partially of eye photoreceptor cell | decreased number phenotype of Tace¹

(Muliyil et al., 2020)

NOT Suppressor of

Statement

Reference

bmm^UAS.cGa/Scer\GAL4^elav.PLu is a non-suppressor of lipid droplet | increased number | adult stage phenotype of Tace¹

(Muliyil et al., 2020)

bmm^UAS.cGa/Scer\GAL4^ninaE.PT is a non-suppressor of eye photoreceptor cell | somatic clone | adult stage phenotype of fh¹

(Chen et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^ninaE.PT fails to rescue the neurodegeneration and electrophysiology defect seen in fh¹ mutant photoreceptor clones.

(Chen et al., 2016)

Expression of bmm^Scer\UAS.cGa in sicily^E mutant clones partially restores the shape, density and number of identifiable rhabdomeres, indicating that reducing lipid droplet accumulation partially alleviates neurodegeneration.

Expression of bmm^Scer\UAS.cGa in Aats-met^FB mutant clones partially restores the shape, density and number of identifiable rhabdomeres, indicating that reducing lipid droplet accumulation partially alleviates neurodegeneration.

Expression of bmm^Scer\UAS.cGa in Marf^B mutant clones partially restores the shape, density and number of identifiable rhabdomeres, indicating that reducing lipid droplet accumulation partially alleviates neurodegeneration.

Neuronal expression of bmm^Scer\UAS.cGa (under the control of Scer\GAL4^elav.PU) suppresses lipid droplet accumulation in Marf^GD11094-knockdown glia.

Neuronal expression of bmm^Scer\UAS.cGa (under the control of Scer\GAL4^elav.PU) suppresses lipid droplet accumulation in Aats-met^KK108492-knockdown glia.

Neuronal expression of bmm^Scer\UAS.cGa (under the control of Scer\GAL4^elav.PU) suppresses lipid droplet accumulation in ND-42^GD6220-knockdown glia.

Knockdown of Marf in neurons (Marf^{ninaE.GD11094}) and overexpression of bmm^Scer\UAS.cGa in glia (under the control of Scer\GAL4^54C) lowers lipid droplet accumulation and delays neurodegeneration.

Knockdown of ND-42 in neurons (ND-42^ninaE.GD6220) and overexpression of bmm^Scer\UAS.cGa in glia (under the control of Scer\GAL4^54C) lowers lipid droplet accumulation and delays neurodegeneration.

(Liu et al., 2015)

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat suppresses the increase in fat content which is seen in Lsd-1¹ flies and the double mutant flies are leaner than flies expressing bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat in a Lsd-1[+] background.

(Beller et al., 2010)

Immature adult flies co-expressing Lsd-2^Scer\UAS.cGa and bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat have storage fat levels (measured as triacylglycerol content) intermediate between control flies and flies expressing bmm^Scer\UAS.cGa alone.

(Gronke et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act5C.PI/bmm^UAS.cGa rescues bmm¹

(Gronke et al., 2005)

Partially rescues

Scer\GAL4^Act5C.PI/bmm^UAS.cGa partially rescues bmm¹

(Gronke et al., 2005)

Comments

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI almost completely rescues the lethality of embryos maternally and zygotically mutant for bmm¹.

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^Act5C.PI reverts the obese phenotype of bmm¹ mutant flies.

(Gronke et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76600

w^*; P{UAS-bmm.cGa}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

bmm^Scer\UAS.cGa

bmm^UAS.cGa

Name Synonyms

Secondary FlyBase IDs

References (22)

Report Sections

Open Close