FlyBase Allele Report: Dmel\Hs3st-B[RNAi.UAS]

General Information

Symbol

Dmel\Hs3st-B^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0195979

Feature type

allele

Associated gene

Dmel\Hs3st-B

Associated Insertion(s)

Carried in Construct

P{UAS-Hs3st-B.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Kamimura et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Kamimura et al., 2004)

Mutagen

in vitro construct

(Kamimura et al., 2004, Kamimura et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Hs3st-B.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat (in a head-to-head orientation) of a 500 bp cDNA fragment from the first methionine codon of Hs3st-B.

(Kamimura et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hs3st-B

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage, with Scer\GAL4^Act.PU

(Kamimura et al., 2004)

lethal | larval stage, with Scer\GAL4^Act.PU

(Kamimura et al., 2004)

visible, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004, Kamimura et al., 2004)

visible, with Scer\GAL4^GMR.PF

(Kamimura et al., 2004)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004, Kamimura et al., 2004)

anterior scutellar bristle, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

campaniform sensillum of anterior crossvein | increased number, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

campaniform sensillum of dorsal radius | increased number, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

endosome, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

eye, with Scer\GAL4^GMR.PF

(Kamimura et al., 2004)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Kamimura et al., 2004)

leg sensillum | increased number, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

lysosome, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

macrochaeta | increased number, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

mesonotum, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Kamimura et al., 2004)

posterior scutellar bristle, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

sternopleural bristle | increased number, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

tarsal segment, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

wing, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

wing margin, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

wing vein L3, with Scer\GAL4^dpp.blk1

(Kamimura et al., 2004, Kamimura et al., 2004)

Detailed Description

Statement

Reference

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^GMR.PS does not affect development of the eye.

(Nishihara, 2010)

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M6 (P{UAS-Hs3st-B.IR}M6) under the control of Scer\GAL4^dpp.blk1 results in thickened longitudinal vein L3, reduction of the anterior crossvein, and macrochaete duplication. These phenotypes are milder than those caused by expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M3 (P{UAS-Hs3st-B.IR}M3.

(Kamimura et al., 2004)

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M3 (P{UAS-Hs3st-B.IR}M3) under the control of Scer\GAL4^GMR.PF causes a severe rough eye phenotype, including necrotic death and an irregular array or fusion of ommatidia, and duplication or loss of interommatidial bristles.

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M3 (P{UAS-Hs3st-B.IR}M3) under the control of Scer\GAL4^dpp.blk1 produces numerous ectopic sensory bristles (macrochaetae) on the mesothoracic notum, reduction in wing size, loss of the anterior cross vein, thickening of wing vein L3, extra or ectopic sensory organs on the anterior cross vein and vein L3, notching of the wing margin, extra sternopleural sensory bristles, extra sensory bristles on legs, and fusions between tarsal segments leading to shortened legs.

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M3 (P{UAS-Hs3st-B.IR}M3) under the control of Scer\GAL4^dpp.blk1 produces numerous sensory organ mother cells (SMCs) from each proneural cluster (as revealed by Ecol\lacZ^neur-A101 expression) whereas a single SMC is singled-out from each proneural cluster in control animals.

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} line M3 (P{UAS-Hs3st-B.IR}M3) under the control of Scer\GAL4^dpp.blk1 increases the number and size of endosomal and lysosomal compartments.

(Kamimura et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has visible phenotype, enhanceable by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has visible phenotype, enhanceable by dx¹

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has visible phenotype, enhanceable by kuz^e29-4

(Kamimura et al., 2004)

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of visible phenotype of Delta^RevF10

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 is an enhancer of visible phenotype of Scer\GAL4^dpp.blk1, sca^UAS.cEa

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of visible phenotype of N^55e11

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of visible phenotype of N^l1N-ts1

(Kamimura et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has wing vein L3 phenotype, enhanceable by sca^UAS.cEa, Scer\GAL4^dpp.blk1

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has wing vein L3 phenotype, enhanceable by dx¹

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has anterior scutellar bristle phenotype, enhanceable by dx¹

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has posterior scutellar bristle phenotype, enhanceable by dx¹

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has wing vein L3 phenotype, enhanceable by kuz^e29-4

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 has anterior scutellar bristle phenotype, enhanceable by kuz^e29-4

(Kamimura et al., 2004)

Enhancer of

Statement

Reference

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of wing margin phenotype of N^55e11

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of wing vein L3 phenotype of N^55e11

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of anterior scutellar bristle phenotype of Delta^RevF10

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of posterior scutellar bristle phenotype of Delta^RevF10

(Kamimura et al., 2004)

Hs3st-B^RNAi.UAS, Scer\GAL4^dpp.blk1 is an enhancer of wing margin phenotype of Scer\GAL4^dpp.blk1, sca^UAS.cEa

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of anterior scutellar bristle phenotype of N^55e11

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of posterior scutellar bristle phenotype of N^55e11

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of wing vein L3 phenotype of N^l1N-ts1

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of anterior scutellar bristle phenotype of N^l1N-ts1

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of posterior scutellar bristle phenotype of N^l1N-ts1

(Kamimura et al., 2004)

Scer\GAL4^dpp.blk1/Hs3st-B^RNAi.UAS is an enhancer of wing vein L3 phenotype of Delta^RevF10

(Kamimura et al., 2004)

Other

Statement

Reference

Hs3st-B^RNAi.UAS, Papst2^RNAi.UAS, Scer\GAL4^GMR.PS has eye phenotype

(Nishihara, 2010)

Hs3st-B^RNAi.UAS, Scer\GAL4^GMR.PS, sll^RNAi.UAS has eye phenotype

(Nishihara, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of Hs3st-B^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^Act produces Hs3st-B dsRNA, resulting in dsRNA interference (RNAi) and a reduction in Hs3st-B transcript levels.

(Kamimura et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Hs3st-B^RNAi.IR.UAS

Hs3st-B^RNAi.UAS

Hs3st-B^{dsRNA.IR.Scer\UAS}

Hs3st-B^dsRNA.IR.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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