Allele Dmel\SPE^{dsRNA.IR.Scer\UAS}

General Information
Symbol	Dmel\SPE^{dsRNA.IR.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0196029
Feature type	allele	Associated gene	Dmel\SPE

Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Kambris et al., 2006) in vitro construct - RNAi (Kambris et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a 500bp cDNA fragment from the 5' end of the spe coding sequence, inserted as an inverted repeat (IR) in a modified pUAST vector, pUAST-R57, which includes a 282bp genomic fragment of ret, containing introns 5 and 6 between the two IR fragments, to enhance the effect of RNAi. (Kambris et al., 2006)
Carried in construct	P{UAS-spe.IR} (Kambris et al., 2006, Kambris et al., 2006, Paddibhatla et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^c564 (Kambris et al., 2006, Paddibhatla et al., 2010, Kambris et al., 2006, Paddibhatla et al., 2010) lethal, with Scer\GAL4^da.G32 (Kambris et al., 2006)
Phenotype Manifest In

Detailed Description
	Statement Reference Animals expressing SPE[dsRNA.IR.Scer\UAS] under the control of Scer\GAL4[c564] are immune-compromised and cannot encapsulate injected wasp eggs. (Paddibhatla et al., 2010) Expression of spe^{dsRNA.IR.Scer\UAS}, under the control of Scer\GAL4^c564 does not affect activation of melanization during pupariation, nor does it suppress lethality induced by the inactivation of Spn27A. (Kambris et al., 2006) Flies expressing spe^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^c564 exhibit an immune-deficiency phenotype similar to Tl pathway mutants: these flies are highly susceptible to Enterococcus faecalis (gram-positive) and fail to properly induce Drs after infection with Micrococcus luteus (gram-positive). Flies expressing spe^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^c564 exhibit an increased susceptibility to fungal infections. (Kambris et al., 2006)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	spe^{dsRNA.IR.Scer\UAS} SPE^{dsRNA.IR.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Paddibhatla et al., 2010, PLoS Pathog. 6(12): e1001234 Role for sumoylation in systemic inflammation and immune homeostasis in Drosophila larvae. [FBrf0212643] Kambris et al., 2006, Curr. Biol. 16(8): 808--813 Drosophila immunity: a large-scale in vivo RNAi screen identifies five serine proteases required for Toll activation. [FBrf0190023]
Supplementary material	Kambris et al., 2006, Curr. Biol. 16(8): Document S1. Two Figures and Supplemental Experimental Procedures. [FBrf0199186]

Allele Dmel\SPEdsRNA.IR.Scer\UAS

Allele Dmel\SPE^{dsRNA.IR.Scer\UAS}