FB2021_01, released Feb 18, 2021

Allele: Dmel\DifdsRNA.IR.UAS

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General Information
Symbol
Dmel\DifdsRNA.IR.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0196036
Feature type
allele
Associated gene
Dmel\Dif
Associated Insertion(s)
Carried in Construct
P{UAS-Dif.IR}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - RNAi
(Kambris et al., 2006)
in vitro construct - regulatory fusion
(Kambris et al., 2006)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Dif.IR}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of an inverted repeat of a 500bp cDNA fragment from the 5' end of the Dif coding sequence.

      (Kambris et al., 2006)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Dif
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4c564 blocks the normal inflammatory response to Gram-positive bacterial infection (assessed by the expression levels of Drosomycin), but has no significant effect in basal (non-infected) conditions, as compared to controls.

      (Kumar et al., 2016)

      Flies expressing DifdsRNA.IR.Scer\UAS, under the control of Scer\GAL4da.G32 exhibit impaired immune response . They exhibit a dramatic loss of survival rate after infection with E.faecalis (gram-positive bacterium). This is mirrored to a lesser extent, when these flies are infected with B.bassiana (filamentous fungus) or C.albicans (yeast).

      (Kambris et al., 2006)

      Adult flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 show a reduced survival rate compared to control flies after bacterial (E.faecalis) infection.

      (Tang et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of either MP1dsRNA.Scer\UAS.WIZ or Sp7dsRNA.Scer\UAS.WIZ results in a further decrease in the survival rate of flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 after fungal (C.albicans) infection.

      (Tang et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      DifdsRNA.IR.Scer\UAS
      DifdsRNA.IR.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (3)