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General Information
Symbol
Dmel\Pis1
Species
D. melanogaster
Name
FlyBase ID
FBal0196214
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The 5' UTR, all of exon 1 and part of exon 2 of the Pis gene has been replaced by a w+mW.hs marker.

Insertion components
TI{TI}Pis1
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

PisFV4 mutant flies exhibit a mislocalization of basement membrane proteins in the follicle cells.

PisFV4 mutant follicle cells frequently form multiple layers or gaps in the epithelial sheet instead of maintaining a strictly monolayered epithelium, indicating a role in epithelial morphogenesis.

Pis1 mutant salivary gland cell clones do not accumulate large numbers of lipid droplets. These clones are significantly smaller than wild-type cells, being on average only about 25% of the size.

Pis1 mutant fat body cells are about 30% smaller on average than control cells.

Mosaic flies with homozygous Pis1 eyes in otherwise heterozygous flies show rough eyes of reduced size. These eyes have no detectable photoreceptors within the ommatidia.

Expression of Pishs.T:Hsap\MYC between the embryonic and the late pupal stages by daily heat-shock treatments allows Pis1 flies to survive to adult stages. When newly-eclosed Pis1; Pishs.T:Hsap\MYC flies are dark adapted for 2 minutes and then exposed to three pulses of orange light, the ERG amplitudes produced are similar to wild-type flies. However, when the same flies are exposed to this light regimen after 3 days without heat shock, the ERG amplitude resulting from the second light pulse is decreased compared with the first. When flies are tested after 7 days without heat shock, amplitudes are further decreased and a very small photoresponse is observed during exposure to the third light pulse.

The ERG recordings of newly-eclosed Pis1; Pishs.T:Hsap\MYC flies exposed to 2 minutes of orange light, followed by a 3 s dark adaptation are not significantly different to those produced by wild-type flies. 3-day-old Pis1; Pishs.T:Hsap\MYC flies (which have no further heat shock after eclosion) show a much smaller ERG amplitude after the 2 min light adaptation, which is restored after 1.5-2 minutes of dark adaptation. 7-day-old Pis1; Pishs.T:Hsap\MYC flies require a minimum of 2 min of dark adaptation to recover the light response.

When newly-eclosed Pis1; Pishs.T:Hsap\MYC flies are reared in the dark without additional heat shocks for 7 days and subsequently exposed to a 12 h light/dark cycle the eyes undergo a gradual loss of rhabdomeres. By 14 days, the rhabdomeres are either missing or dramatically smaller than in wild type and are distorted in appearance. This rhabdomere degeneration phenotype is not seen when newly eclosed Pis1; Pishs.T:Hsap\MYC flies are maintained in the dark. Induction of daily heat-shock treatments after day 7 in Pis1; Pishs.T:Hsap\MYC flies reared in a light/dark cycle prevents rhabdomere loss.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Expression of Hsap\CDIPTPis.PW rescues both the embryonic lethality and the eye phenotype of Pis1 flies. Rescue of eyes is complete as the ERG response of rescued flies is indistinguishable from wild type.

Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)