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General Information
Symbol
Dmel\Eps-15Δ29
Species
D. melanogaster
Name
FlyBase ID
FBal0196828
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
eps15Δ29
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

A deletion of Eps-15 that has been isolated by Scer\FLP1-mediated recombination between the P{XP}Eps-15d00130 and PBac{WH}f02085 insertions. The resulting recombination insertion is P+PBac{XP3.WH3}Eps-15Δ29.

Deletion of the entire Eps-15 coding region.

FlyBase curator comment: one of the progenitor insertions given in FBrf0202144 for the Eps-15Δ29 allele is incorrect. Please see FBrf0206397 for the correct description of the progenitors of the Eps-15Δ29 allele.

Insertion components
P+PBac{XP3.WH3}Eps-15Δ29
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The neuromuscular junctions of Eps-15e75/Eps-15Δ29 third instar larvae show impaired FM1-43FX dye uptake compared to wild type, indicating a defect in synaptic vesicle endocytosis.

The average number of boutons per neuromuscular junction is increased in Eps-15e75/Eps-15Δ29 and Eps-15Δ29/Df(2R)Dll-MP mutant larvae. In addition, the mutant neuromuscular junctions have more boutons with two branches and more boutons with three or more branches than controls.

Spontaneous miniature excitatory junctional potentials (mEJPs) at the NMJ of Eps-15e75/Eps-15Δ29 larvae have a similar amplitude, but higher frequency, than those of controls. When the NMJ of Eps-15e75/Eps-15Δ29 larvae is stimulated at a low frequency, evoked EJPs have a similar amplitude to those of controls. However, under high-frequency stimulation conditions (10Hz for 10 minutes), the mutant NMJs show strong synaptic depression to approximately 40% of the initial EJP amplitude (in contrast to control NMJs, which are able to maintain EJPs at approximately 90% of the initial amplitude).

Homozygous and Eps-15e75/Eps-15Δ29 third instar larvae show severely reduced dye uptake at the neuromuscular junction during nerve stimulation compared to controls, indicating a defect in endocytosis.

The mean vesicle density in Eps-15e75/Eps-15Δ29 larval boutons at rest is not significantly different from that of controls, although there is an increased proportion of abnormally large vesicles or cisternae in the resting mutant boutons. During a 90mM K[+] stimulation, the mutant boutons show a reduction in mean vesicle density compared to controls and there is an increased proportion of large cisternae that are greater than 85nm in size (some of these cisternae appear to be large membrane invaginations which are contiguous with the plasma membrane). When the mutant boutons are allowed to recover for 1 minute after stimulation, the mean vesicle density shows a poor recovery, being reduced compared to resting levels (in contrast to wild-type boutons which recover the vesicle density to resting levels). The size distribution of boutons after a 1 minute recovery from stimulation is normal in the mutants.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The introduction of Eps-15Δ29 into stmA1/stmArev499 animals does not cause detectable behavioral effects at permissive or restrictive rearing temperatures.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

T.W. Koh

H.J. Bellen

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)