Allele Scer\GAL4^Ras64B.PW

General Information
Symbol	Scer\GAL4^Ras64B.PW	Species	S. cerevisiae
Name	Ras oncogene at 64B promoter construct of Walker	FlyBase ID	FBal0197136
Feature type	allele	Associated gene	Scer\GAL4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Walker et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A 358bp segment of the Ras64B promoter region drives expression of Scer\GAL4. (Walker et al., 2006)
Carried in construct	P{Ras64B-GAL4.W} (Walker et al., 2006, Gouzi et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| adult stage, with Alk^{act.Scer\UAS.T:Ivir\HA1} (Gouzi et al., 2011) decreased cell size \| adult stage, with Alk^Scer\UAS.cLa (Gouzi et al., 2011) increased cell size \| adult stage, with Alk^{EC.Scer\UAS.T:Hsap\MYC} (Gouzi et al., 2011) increased cell size \| adult stage, with Alk^GD42 (Gouzi et al., 2011) learning defective \| adult stage, with Alk^{act.Scer\UAS.T:Ivir\HA1}, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Alk^{EC.Scer\UAS.T:Hsap\MYC}, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Alk^GD42, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Alk^Scer\UAS.cLa, Scer\GAL80^ts.αTub84B (Gouzi et al., 2011) learning defective \| adult stage, with Scer\GAL80^ts.αTub84B, jeb^Scer\UAS.cVa (Gouzi et al., 2011) lethal \| pupal stage \| heat sensitive, with Ras64B^{Val14.Scer\UAS} (Walker et al., 2006) lethal \| pupal stage \| heat sensitive, with Ras85D^{V12.cGa.Scer\UAS} (Walker et al., 2006) size defective \| pupal stage, with Alk^{act.Scer\UAS.T:Ivir\HA1} (Gouzi et al., 2011) size defective \| pupal stage, with Alk^{EC.Scer\UAS.T:Hsap\MYC} (Gouzi et al., 2011) size defective \| pupal stage, with Alk^GD42 (Gouzi et al., 2011) size defective \| pupal stage, with Alk^Scer\UAS.cLa (Gouzi et al., 2011) size defective \| pupal stage, with jeb^Scer\UAS.cVa (Gouzi et al., 2011) size defective \| pupal stage, with rl^Sem.Scer\UAS (Gouzi et al., 2011) small body \| adult stage, with phl^{Scer\UAS.F179} (Walker et al., 2006) small body \| adult stage, with Ras64B^{Val14.Scer\UAS} (Walker et al., 2006) small body \| adult stage, with Ras85D^{V12.cGa.Scer\UAS} (Walker et al., 2006)
Phenotype Manifest In
	wing, with Alk^{act.Scer\UAS.T:Ivir\HA1} (Gouzi et al., 2011) wing, with Alk^{EC.Scer\UAS.T:Hsap\MYC} (Gouzi et al., 2011) wing, with Alk^GD42 (Gouzi et al., 2011) wing, with Alk^Scer\UAS.cLa (Gouzi et al., 2011)
Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ras64B.PW is a suppressor \| partially of size defective \| recessive \| pupal stage phenotype of Nf1^E2 (Gouzi et al., 2011) Alk^{EC.Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ras64B.PW is a suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011) Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor \| partially of decreased cell size \| adult stage phenotype of Nf1^E2 (Gouzi et al., 2011) Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor \| partially of size defective \| recessive \| larval stage phenotype of Nf1^E2 (Gouzi et al., 2011) Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor of learning defective \| adult stage phenotype of Nf1^E1/Nf1^E2 (Gouzi et al., 2011) vap^{Scer\UAS.T:Hsap\MYC}/Scer\GAL4^Ras64B.PW is a suppressor of small body \| pupal stage phenotype of Nf1^E2 (Walker et al., 2006)
NOT Suppressor of
Statement Reference Scer\GAL4^Ras64B.PW/RasGAP1^Scer\UAS.cWa is a non-suppressor of small body \| pupal stage phenotype of Nf1^E2 (Walker et al., 2006)
Phenotype Manifest In
Suppressor of
Statement Reference Alk^GD42/Scer\GAL4^Ras64B.PW is a suppressor \| partially of wing phenotype of Nf1^E2 (Gouzi et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Nf1^{GRD.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^Ras64B.PW rescues Nf1^E2 (Walker et al., 2006) Nf1^Scer\UAS.cWa, Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B rescues Nf1^E1/Nf1^E2 (Gouzi et al., 2011) Nf1^Scer\UAS.cWa/Scer\GAL4^Ras64B.PW rescues Nf1^E2 (Walker et al., 2006)
Fails to rescue	Scer\GAL4^Ras64B.PW/Nf1^{R1320P.GRD.Scer\UAS.T:Ivir\HA1} fails to rescue Nf1^E2 (Walker et al., 2006)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Scer\GAL4^Ras64B.PW
Name Synonym	Ras oncogene at 64B promoter construct of Walker
Secondary FlyBase IDs

References ( 2 )
Research paper	Gouzi et al., 2011, PLoS Genet. 7(9): e1002281 The receptor tyrosine kinase alk controls neurofibromin functions in Drosophila growth and learning. [FBrf0216258] Walker et al., 2006, Genes Dev. 20(23): 3311--3323 Reduced growth of Drosophila neurofibromatosis 1 mutants reflects a non-cell-autonomous requirement for GTPase-Activating Protein activity in larval neurons. [FBrf0193208]

Allele Scer\GAL4Ras64B.PW

Allele Scer\GAL4^Ras64B.PW