FB2025_01 , released February 20, 2025
Allele: Dmel\Rfx2202-480.UAS.Tag:HA
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General Information
Symbol
Dmel\Rfx2202-480.UAS.Tag:HA
Species
D. melanogaster
Name
FlyBase ID
FBal0197142
Feature type
allele
Associated gene
Rfx2
Associated Insertion(s)
Carried in Construct
P{UAS-Rfx2.HA.202-480}
Key Links
Transgenic product class
inframe_deletion
(Otsuki et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Otsuki et al., 2004)
Mutagen
in vitro construct
(Otsuki et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-Rfx2.HA.202-480}
Cytology
Description

UASt regulatory sequences drive expression of amino acids 202-480 (the putative DNA-binding domain) of Rfx2 tagged with Tag:HA.

(Otsuki et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rfx2
Tagged with
Tag:HA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Rfx2202-480.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4ptc-559.1 results in semi-lethality. Surviving flies have short bristles.

      Expression of one copy of Rfx2202-480.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PS results in a rough eye phenotype. The ommatidia lack their normal regular hexagonal shape and appear to be fused. There is an extensive reduction in BrdU incorporation in the synchronised S-phase zone behind the morphogenetic furrow in eye imaginal discs expressing Rfx2202-480.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PS. There is also a significant increase in the number of apoptotic cells posterior to the morphogenetic furrow in these eye discs compared to controls.

      Expression of Rfx2202-480.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL471B, Scer\GAL4twi.PG or Scer\GAL4Act88F.PD does not result in a detectable mutant phenotype.

      (Otsuki et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      Co-expression of Rfx2Scer\UAS.T:Ivir\HA1 suppresses the rough eye phenotype caused by expression of Rfx2202-480.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PS.

      (Otsuki et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Rfx2202-480.Scer\UAS.T:Ivir\HA1
      Rfx2202-480.UAS.Tag:HA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)