FlyBase Allele Report: Dmel\twin[GS8115]

General Information

Symbol

Dmel\twin^GS8115

Species

D. melanogaster

Name

FlyBase ID

FBal0197364

Feature type

allele

Associated gene

Dmel\twin

Associated Insertion(s)

P{GSV3}twin^GS8115

Carried in Construct

Also Known As

twin⁸¹¹⁵

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GSV3}twin^GS8115

Cytology

Description

Insertion in intron 5. The orientation of the insertion is the same as the direction of transcription of twin.

(Zaessinger et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{GSV3}

misexpression element

Regulatory region(s)

UASt

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:24,206,555..24,206,555

(Zaessinger et al., 2006, Benoit et al., 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates oculopharyngeal muscular dystrophy

modeled by Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

modeled by Hsap\PABPN1^17ala.UAS

(Chartier et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage (with twin^GS12209)

(Pradhan et al., 2012)

female sterile (with Df(3R)Exel6198)

(Zaessinger et al., 2006)

lethal - all die during embryonic stage | maternal effect | partially

(Zaessinger et al., 2006)

some die during embryonic stage | partially

(Zaessinger et al., 2006)

viable

(Zaessinger et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

100% of germaria lack germline stem cells (GSCs) in the ovaries of 7 day old twin^GS8115/Df(3R)Exel6198 females.

Homozygous clonal GSCs are lost more rapidly from the germaria than control germline clones. Division in the mutant GSCs s slower than in wild type.

(Joly et al., 2013)

twin^GS8115/twin^GS12209 larvae show a significant increase in the number of type 1b boutons at the muscle 6/7 neuromuscular junction compared to controls.

(Pradhan et al., 2012)

Embryos derived from twin^GS8115/twin^GS8115 females mated to wild-type males show 78% lethality.

No eggs are produced from a cross of twin^GS8115/Df(3R)Exel6198 females mated to wild-type males.

Homozygous females show a number of ovarian defects; 6% of egg chambers are wild-type, but 34% have less than 15 nurse cells, 36% have more than 15 nurse cells and 24% show degeneration.

twin^GS8115/Df(3R)crb-F89-4 females show a number of ovarian defects; 14% of egg chambers are wild-type, but 20% have less than 15 nurse cells, 20% have more than 15 nurse cells, 43.5% show degeneration and 2.5% have other defects.

(Zaessinger et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

twin[+]/twin^GS8115 is a suppressor of visible phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2015)

twin[+]/twin^GS8115 is a suppressor of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Other

Statement

Reference

Patr-1^d3, twin[+]/twin^GS8115 has abnormal neuroanatomy | third instar larval stage phenotype

(Pradhan et al., 2012)

smg[+]/smg¹, twin^GS8115 has lethal | dominant | maternal effect | embryonic stage phenotype

(Zaessinger et al., 2006)

Pabp2[+]/Pabp2⁵⁵, twin^GS8115 has partially lethal - majority die | recessive | maternal effect | embryonic stage phenotype

(Benoit et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

twin[+]/twin^GS8115 is a suppressor of wing phenotype of Hsap\PABPN1^17ala.UAS, Scer\GAL4^Mhc.PW

(Chartier et al., 2015)

twin[+]/twin^GS8115 is a suppressor of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Other

Statement

Reference

Patr-1^d3, twin[+]/twin^GS8115 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Pradhan et al., 2012)

Patr-1^d3, twin[+]/twin^GS8115 has type Ib terminal bouton | increased number | third instar larval stage phenotype

(Pradhan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

twin^GS8115/+ ; Patr-1^d3/+ double heterozygous larvae show a significant increase in the number of type 1b boutons at the muscle 6/7 neuromuscular junction compared to controls.

(Pradhan et al., 2012)

Embryos derived from twin^GS8115 smg¹ double heterozygotes show 32% lethality, whereas embryos derived from either single mutant do not show maternal effect lethality.

(Zaessinger et al., 2006)

Over 40% of eggs laid by twin^GS8115/+, Pabp2⁵⁵/+ females fail to hatch.

(Benoit et al., 2005)

Xenogenetic Interactions

Statement

Reference

twin^GS8115 suppresses the wing posture defects seen upon expression of Hsap\PABPN1^17ala.Act88F.

twin^GS8115 suppresses the wing posture defects seen when Hsap\PABPN1^{17ala.Scer\UAS} is expressed under the control of Scer\GAL4^Mhc.PW. The number of nuclei containing aggregates increases in the presence of twin^GS8115, although the cross-sectional area remains the same.

(Chartier et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

201401

y¹ w^67c23; P{GSV3}twin^GS8115/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ccr4⁸¹¹⁵

(Benoit et al., 2005)

twin⁸¹¹⁵

(Chartier et al., 2015, Rojas-Ríos et al., 2015, Joly et al., 2013, Zaessinger et al., 2006)

twin^GS8115

(Pradhan et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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