Allele Dmel\Sp7^{dsRNA.Scer\UAS.WIZ}

General Information
Symbol	Dmel\Sp7^{dsRNA.Scer\UAS.WIZ}	Species	D. melanogaster
Name		FlyBase ID	FBal0197369
Feature type	allele	Associated gene	Dmel\Sp7

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Tang et al., 2006) in vitro construct - RNAi (Tang et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat of approximately 600bp of Sp7 sequence. (Tang et al., 2006)
Carried in construct	P{UAS-Sp7.WIZ} (Tang et al., 2006, Tang et al., 2008, Nam et al., 2012)
Cytology
Phenotypic Data
Phenotypic Class
	immune response defective, with Scer\GAL4^da.G32 (Tang et al., 2006) lethal, with Scer\GAL4^Act.PU (Tang et al., 2006) wound healing defective, with Scer\GAL4^Act.PU (Tang et al., 2006)
Phenotype Manifest In

Detailed Description
	Statement Reference Flies expressing Sp7[dsRNA.Scer\UAS.WIZ] under the control of Scer\GAL4[da.G32] show no effect on mortality within 24 hours of mild wounding in 7-8 days old adults. Flies expressing Sp7[dsRNA.Scer\UAS.WIZ] under the control of Scer\GAL4[Act5C.PI] show comparable levels of mortality to wild type in response to mild wounding in 7-8 day old adults. (Nam et al., 2012) Approximately 50% of animals carrying Sp7^{dsRNA.Scer\UAS.WIZ} and Scer\GAL4^Act die at the late pupal stage or during eclosion. Adults expressing Sp7^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^Act do not show melanisation around a wound site a few hours after infection by piercing with a tungsten needle harboring E.coli and M.luteus, in contrast to control flies which show significant melanisation around the wound site. Adult flies expressing Sp7^{dsRNA.Scer\UAS.WIZ} under the control of Scer\GAL4^da.G32 show a similar survival rate to control flies after bacterial (E.carotovora, E.faecalis) infection, although their survival is somewhat reduced compared to controls after natural fungal infection (B.bassiana). (Tang et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Sp7^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^da.G32 is an enhancer of immune response defective phenotype of Fadd^{dsRNA.Scer\UAS}, Scer\GAL4^da.G32 (Tang et al., 2006)
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Sp7^{dsRNA.Scer\UAS.WIZ} is a suppressor of lethal \| larval stage phenotype of Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} (Tang et al., 2008)
NOT Suppressor of
Statement Reference Sp7^{dsRNA.Scer\UAS.WIZ}, Scer\GAL4^Act.PU is a non-suppressor of melanotic mass phenotype \| larval stage phenotype of MP1^{Scer\UAS.P\T.T:SS-ea}, Scer\GAL4^Act.PU (Tang et al., 2006)
Phenotype Manifest In
Suppressor of
Statement Reference Scer\GAL4^Act.PU/Sp7^{dsRNA.Scer\UAS.WIZ} is a suppressor of tracheal system phenotype of Scer\GAL4^Act.PU, Spn77Ba^{dsRNA.WIZ.Scer\UAS} (Tang et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Sp7^{dsRNA.Scer\UAS.WIZ} results in a further decrease in the survival rate of flies expressing Dif^{dsRNA.IR.Scer\UAS} under the control of Scer\GAL4^da.G32 after fungal (C.albicans) infection. Co-expression of Sp7^{dsRNA.Scer\UAS.WIZ} results in a moderate further decrease in the survival rate of flies expressing BG4^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 after bacterial (E.carotovora) infection. (Tang et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	dsRNA is expressed in transgenic flies to study the phenotypic consequences of dsRNA interference (RNAi) of the Sp7 gene. (Tang et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Sp7^{dsRNA.Scer\UAS.WIZ}
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Nam et al., 2012, EMBO J. 31(5): 1253--1265 Genetic evidence of a redox-dependent systemic wound response via Hayan Protease-Phenoloxidase system in Drosophila. [FBrf0217593] Tang et al., 2008, Dev. Cell 15(4): 617--626 A serpin that regulates immune melanization in the respiratory system of Drosophila. [FBrf0206001] Tang et al., 2006, J. Biol. Chem. 281(38): 28097--28104 Two proteases defining a melanization cascade in the immune system of Drosophila. [FBrf0194554]

Allele Dmel\Sp7dsRNA.Scer\UAS.WIZ

Allele Dmel\Sp7^{dsRNA.Scer\UAS.WIZ}