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General Information
Symbol
Dmel\Sp7dsRNA.UAS.WIZ
Species
D. melanogaster
Name
FlyBase ID
FBal0197369
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat of approximately 600bp of Sp7 sequence.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 show no effect on mortality within 24 hours of mild wounding in 7-8 days old adults.

Flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Act5C.PI show comparable levels of mortality to wild type in response to mild wounding in 7-8 day old adults.

Approximately 50% of animals carrying Sp7dsRNA.Scer\UAS.WIZ and Scer\GAL4Act die at the late pupal stage or during eclosion. Adults expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4Act do not show melanisation around a wound site a few hours after infection by piercing with a tungsten needle harboring E.coli and M.luteus, in contrast to control flies which show significant melanisation around the wound site.

Adult flies expressing Sp7dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4da.G32 show a similar survival rate to control flies after bacterial (E.carotovora, E.faecalis) infection, although their survival is somewhat reduced compared to controls after natural fungal infection (B.bassiana).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Sp7dsRNA.Scer\UAS.WIZ results in a further decrease in the survival rate of flies expressing DifdsRNA.IR.Scer\UAS under the control of Scer\GAL4da.G32 after fungal (C.albicans) infection.

Co-expression of Sp7dsRNA.Scer\UAS.WIZ results in a moderate further decrease in the survival rate of flies expressing BG4dsRNA.Scer\UAS under the control of Scer\GAL4da.G32 after bacterial (E.carotovora) infection.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

dsRNA is expressed in transgenic flies to study the phenotypic consequences of dsRNA interference (RNAi) of the Sp7 gene.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Sp7dsRNA.Scer\UAS.WIZ
Sp7dsRNA.UAS.WIZ
Name Synonyms
Secondary FlyBase IDs
    References (3)