Allele Dmel\Uba1^B2

General Information
Symbol	Dmel\Uba1^B2	Species	D. melanogaster
Name		FlyBase ID	FBal0197418
Feature type	allele	Associated gene	Dmel\Uba1

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Pfleger et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:5,577,255..5,577,255 na_change=C5577255T pr_change=P884L \| Uba1-PA reported_pr_change=P884L comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. (Pfleger et al., 2007)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: P884L. (Pfleger et al., 2007)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell number (Yan et al., 2009) increased cell number (with Uba1^B1) (Yan et al., 2009) visible \| somatic clone (Pfleger et al., 2007)
Phenotype Manifest In
	eye \| somatic clone (Pfleger et al., 2007) eye disc posterior to the morphogenetic furrow (Yan et al., 2009) eye disc posterior to the morphogenetic furrow (with Uba1^B1) (Yan et al., 2009) retina \| P-stage \| somatic clone (Pfleger et al., 2007)
Detailed Description
	Statement Reference Uba1[B2] homozygous and Uba1[B1]/Uba1[B2] trans-heterozygous eye discs exhibit a significant increase in proliferation (as observed through phosphorylated His3 signal). Ectopic proliferation posterior to the morphogenetic furrow is significant enough that the region of frequent anti-pHis3 staining extends two to three times as far into the region posterior to the morphogenetic furrow. (Yan et al., 2009) Homozygous clones in the eye have a growth advantage compared to surrounding wild-type tissue at 18^oC. At 30^oC, homozygous clones in the eye result in overgrowth of surrounding wild-type tissue. Homozygous clones in the pupal retina 46 hours after puparium formation contain additional interommatidial cells compared to control retinas (where these extra cells are eliminated by apoptosis during the mid-pupal phase). (Pfleger et al., 2007)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Ras85D^e1B/Ras85D[+], Uba1^B1/Uba1^B2 has viable phenotype, suppressible by egr^Scer\UAS.cMa/Scer\GAL4^GMR.PF (Yan et al., 2009) Uba1^B1/Uba1^B2 has increased cell number phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2009) Uba1^B1/Uba1^B2 has lethal phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2009) Uba1^B2 has increased cell number phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2009)
NOT suppressed by
Statement Reference Uba1^B1/Uba1^B2 has increased cell number phenotype, non-suppressible by drk^k02401/drk[+] (Yan et al., 2009) Uba1^B1/Uba1^B2 has increased cell number phenotype, non-suppressible by Egfr[+]/Egfr^k05115 (Yan et al., 2009) Uba1^B1/Uba1^B2 has increased cell number phenotype, non-suppressible by Sos^e4G/Sos[+] (Yan et al., 2009) Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by drk^k02401/drk[+] (Yan et al., 2009) Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by Egfr[+]/Egfr^k05115 (Yan et al., 2009) Uba1^B1/Uba1^B2 has lethal phenotype, non-suppressible by Sos^e4G/Sos[+] (Yan et al., 2009)
Suppressor of
Statement Reference Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of visible phenotype of grim^GMR.PC (Pfleger et al., 2007, Pfleger et al., 2007) Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^Scer\UAS.cMa (Pfleger et al., 2007, Pfleger et al., 2007) Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of visible phenotype of W^GMR.PG/W^GMR.PG (Pfleger et al., 2007, Pfleger et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference Uba1^B1/Uba1^B2 has eye disc posterior to the morphogenetic furrow phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2009)
Suppressor of
Statement Reference Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of eye phenotype of grim^GMR.PC (Pfleger et al., 2007, Pfleger et al., 2007) Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, egr^Scer\UAS.cMa (Pfleger et al., 2007, Pfleger et al., 2007) Uba1^B2/Uba1^B2 is a suppressor \| partially \| somatic clone of eye phenotype of W^GMR.PG/W^GMR.PG (Pfleger et al., 2007, Pfleger et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The cell-autonomous overgrowth phenotype found in Uba1[B2] homozygous and Uba1[B1]/Uba1[B2] trans-heterozygous eye discs is suppressed in a Ras85D[e1B] heterozygous background. A Ras85D[e1B]/+ background dramatically suppresses the lethality found in Uba1[B1]/Uba1[B2] homozygotes. Uba1[B1]/Uba1[B2] eyes in a Ras85D[e1B] heterozygous background show decreased suppression of egr[Scer\UAS.cMa] (under the control of Scer\GAL4[GMR.PF]) induced cell death. Overall cell proliferation in Uba1[B1]/Uba1[B2] eye discs is reduced in a Ras85D[e1B]/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe. A Egfr[k05115]/+, drk[k02401]/+, or Sos[e4G]/+ background does not suppress the ectopic cell divisions found in Uba1[B1]/Uba1[B2] trans-heterozygous eye discs. (Yan et al., 2009) The loss of eye tissue that is caused by expression of egr^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF is partially suppressed if the eye is also homozygous for Uba1^B2. The loss of eye tissue that is caused by W^GMR.PG is partially suppressed if the eye is also homozygous for Uba1^B2. The loss of eye tissue that is caused by grim^GMR.PC is partially suppressed if the eye is also homozygous for Uba1^B2. (Pfleger et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Uba1⁰³⁴⁰⁵ (Pfleger et al., 2007) Uba1^s3484 (Pfleger et al., 2007)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Uba1^B2 (Pfleger et al., 2007, Yan et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Yan et al., 2009, J. Cell Sci. 122(9): 1461--1470 Impairment of ubiquitylation by mutation in Drosophila E1 promotes both cell-autonomous and non-cell-autonomous Ras-ERK activation in vivo. [FBrf0207806] Pfleger et al., 2007, Fly 1(2): 95--105 Mutation of the gene encoding the ubiquitin activating enzyme Uba1 causes tissue overgrowth in Drosophila. [FBrf0200069]

Allele Dmel\Uba1B2

Allele Dmel\Uba1^B2