|Feature type||allele||Associated gene||Dmel\Uba1|
|Map ( GBrowse )|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: P884L.
|Phenotype Manifest In|
Uba1[B2] homozygous and Uba1[B1]/Uba1[B2] trans-heterozygous eye discs exhibit a significant increase in proliferation (as observed through phosphorylated His3 signal). Ectopic proliferation posterior to the morphogenetic furrow is significant enough that the region of frequent anti-pHis3 staining extends two to three times as far into the region posterior to the morphogenetic furrow.
Homozygous clones in the eye have a growth advantage compared to surrounding wild-type tissue at 18oC. At 30oC, homozygous clones in the eye result in overgrowth of surrounding wild-type tissue. Homozygous clones in the pupal retina 46 hours after puparium formation contain additional interommatidial cells compared to control retinas (where these extra cells are eliminated by apoptosis during the mid-pupal phase).
Ras85De1B/Ras85D[+], Uba1B1/Uba1B2 has viable phenotype, suppressible by egrScer\UAS.cMa/Scer\GAL4GMR.PF
|NOT suppressed by|
Uba1B2/Uba1B2 is a suppressor | partially | somatic clone of visible phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrScer\UAS.cMa
|Phenotype Manifest In|
Uba1B2/Uba1B2 is a suppressor | partially | somatic clone of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, egrScer\UAS.cMa
The cell-autonomous overgrowth phenotype found in Uba1[B2] homozygous and Uba1[B1]/Uba1[B2] trans-heterozygous eye discs is suppressed in a Ras85D[e1B] heterozygous background. A Ras85D[e1B]/+ background dramatically suppresses the lethality found in Uba1[B1]/Uba1[B2] homozygotes. Uba1[B1]/Uba1[B2] eyes in a Ras85D[e1B] heterozygous background show decreased suppression of egr[Scer\UAS.cMa] (under the control of Scer\GAL4[GMR.PF]) induced cell death. Overall cell proliferation in Uba1[B1]/Uba1[B2] eye discs is reduced in a Ras85D[e1B]/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe. A Egfr[k05115]/+, drk[k02401]/+, or Sos[e4G]/+ background does not suppress the ectopic cell divisions found in Uba1[B1]/Uba1[B2] trans-heterozygous eye discs.
The loss of eye tissue that is caused by expression of egrScer\UAS.cMa under the control of Scer\GAL4GMR.PF is partially suppressed if the eye is also homozygous for Uba1B2. The loss of eye tissue that is caused by WGMR.PG is partially suppressed if the eye is also homozygous for Uba1B2. The loss of eye tissue that is caused by grimGMR.PC is partially suppressed if the eye is also homozygous for Uba1B2.
|Complementation & Rescue Data|
|Fails to complement|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 1 )|
|Secondary FlyBase IDs|
|References ( 2 )|