|Name||Saccharomyces cerevisiae UAS construct of Merianda||FlyBase ID||FBal0197449|
|Feature type||allele||Associated gene||Dmel\ptc|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
|Phenotype Manifest In|
|Complementation & Rescue Data|
Maternally-derived expression of ptcScer\UAS.cMa under the control of Scer\GAL4arm.PS in a ptcunspecified mutant background rescues the formation of NB4-2 and specification defects in as many as 60% of hemisegments. With Fas2 staining, guidance defects were rescued in 80% of hemisegments. Staining with BP102 revealed 60% rescue of guidance defects per segment, indicating that not all of the neuroblasts that contribute to commissural tracts were rescued. When Scer\GAL4arm.PS is paternally-derived, neither the NB4-2 defects nor the medial tract guidance defects in ptcunspecified mutants are rescued. Expression of ptcScer\UAS.cMa under the control of Scer\GAL4hs.PB for 30 minutes at 37oC results in a near complete rescue of the longitudinal tracts in ptcunspecified embryos. Medial tracts are normal in 93% of hemisegments. Even when there is mis-routing of the medal tracts, only a few growth cones appear to be crossing the midline. These rescues are only observed when the ptcScer\UAS.cMa transgene is induced between 2 and 3 hours of development. No rescue is observed with the induction of ptcScer\UAS.cMa during other developmental time points. A 10 minute induction of the ptcScer\UAS.cMa transgene partially rescues the defects, whereas a 20 minute induction results in a very strong rescue. These results indicate that the presence of ptc protein in a narrow time window during the specification of neuroblast identity is sufficient to rescue the medial axon tract defects in ptcunspecified mutant embryos.
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct of Merianda
|Secondary FlyBase IDs|
|References ( 1 )|