Allele Dmel\ptc^Scer\UAS.cMa

General Information
Symbol	Dmel\ptc^Scer\UAS.cMa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct of Merianda	FlyBase ID	FBal0197449
Feature type	allele	Associated gene	Dmel\ptc

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Merianda et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of ptc coding sequences. (Merianda et al., 2005)
Carried in construct	P{UAS-ptc.M} (Merianda et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	ptc^Scer\UAS.cMa/Scer\GAL4^hs.PB rescues ptc^unspecified (Merianda et al., 2005)
Partially rescues	Scer\GAL4^arm.PS/ptc^Scer\UAS.cMa partially rescues ptc^unspecified (Merianda et al., 2005)
Comments	Maternally-derived expression of ptc^Scer\UAS.cMa under the control of Scer\GAL4^arm.PS in a ptc^unspecified mutant background rescues the formation of NB4-2 and specification defects in as many as 60% of hemisegments. With Fas2 staining, guidance defects were rescued in 80% of hemisegments. Staining with BP102 revealed 60% rescue of guidance defects per segment, indicating that not all of the neuroblasts that contribute to commissural tracts were rescued. When Scer\GAL4^arm.PS is paternally-derived, neither the NB4-2 defects nor the medial tract guidance defects in ptc^unspecified mutants are rescued. Expression of ptc^Scer\UAS.cMa under the control of Scer\GAL4^hs.PB for 30 minutes at 37^oC results in a near complete rescue of the longitudinal tracts in ptc^unspecified embryos. Medial tracts are normal in 93% of hemisegments. Even when there is mis-routing of the medal tracts, only a few growth cones appear to be crossing the midline. These rescues are only observed when the ptc^Scer\UAS.cMa transgene is induced between 2 and 3 hours of development. No rescue is observed with the induction of ptc^Scer\UAS.cMa during other developmental time points. A 10 minute induction of the ptc^Scer\UAS.cMa transgene partially rescues the defects, whereas a 20 minute induction results in a very strong rescue. These results indicate that the presence of ptc protein in a narrow time window during the specification of neuroblast identity is sufficient to rescue the medial axon tract defects in ptc^unspecified mutant embryos. (Merianda et al., 2005)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ptc^Scer\UAS.cMa
Name Synonym	Saccharomyces cerevisiae UAS construct of Merianda
Secondary FlyBase IDs

References ( 1 )
Research paper	Merianda et al., 2005, Dev. Genes Evol. 215(6): 285--296 Patched regulation of axon guidance is by specifying neural identity in the Drosophila nerve cord. [FBrf0187440]

Allele Dmel\ptcScer\UAS.cMa

Allele Dmel\ptc^Scer\UAS.cMa