FlyBase Allele Report: Dmel\Mnn1[e200]

General Information

Symbol

Dmel\Mnn1^e200

Species

D. melanogaster

Name

FlyBase ID

FBal0197542

Feature type

allele

Associated gene

Dmel\Mnn1

Associated Insertion(s)

P{lacW}milt^e200

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Busygina et al., 2004)

Progenitor genotype

P{lacW}milt^k04704

(Busygina et al., 2004)

Associated Insertion(s)

P{lacW}milt^e200

Caused by aberration

Df(2L)Mnn1^e200

Cytology

Description

Imprecise excision of the P{lacW} element, resulting in a 9.5kb deletion that extends from the 3' end of milt to the middle of exon 4 of the Mnn1 gene. The deletion removes more than half of the Mnn1 coding region.

(Busygina et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of multiple endocrine neoplasia type 1

CEA

(Cerrato et al., 2006, Busygina et al., 2004)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal DNA repair

(Busygina et al., 2004)

fertile

(Busygina et al., 2004)

radiation sensitive

(Busygina et al., 2004)

viable

(Busygina et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Mnn1^e200 mutants (Df(2L)Mnn1^e200 mutants in which milt function is rescued by expression of the milt⁺²² transgene) show a greater sensitivity to ionizing radiation when exposed as second and third instar larvae compared to wild-type larvae. As first instar larvae, these Mnn1^e200 mutants show increased sensitivity to the DNA cross-linking agents nitrogen mustard and cisplatinum at room temperature and 29^oC, and increased sensitivity to diepoxybutane and mitomycin C at 29^oC but not at room temperature. These mutants display similar sensitivity to wild-type larvae when exposed to methyl methanosulphonate, hydroxyurea and 2-Acetylaminofluorene.

Mnn1^e200 mutant cells are able to arrest normally at the G2/M transition after irradiation and show indistinguishable levels of apoptosis to controls.

(Busygina et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Mnn1^e200 is a non-enhancer of visible | chemical conditional phenotype of Fancd2^RNAi.UAS, Scer\GAL4^ey.PU

(Marek et al., 2008)

NOT Suppressor of

Statement

Reference

Mnn1^e200 is a non-suppressor of visible | chemical conditional phenotype of Fancd2^RNAi.UAS, Scer\GAL4^ey.PU

(Marek et al., 2008)

Other

Statement

Reference

Mnn1^e200, Scer\GAL4^ey.PU, wts^x1/wts[+] has chemical sensitive phenotype

(Marek et al., 2008)

Mnn1^e200, Scer\GAL4^ey.PU, wts^x1/wts[+] has neoplasia | conditional phenotype

(Marek et al., 2008)

Mnn1^e200, Scer\GAL4^ey.PU, wts^x1/wts[+] has visible | chemical conditional phenotype

(Marek et al., 2008)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Mnn1^e200 is a non-enhancer of eye | chemical conditional phenotype of Fancd2^RNAi.UAS, Scer\GAL4^ey.PU

(Marek et al., 2008)

NOT Suppressor of

Statement

Reference

Mnn1^e200 is a non-suppressor of eye | chemical conditional phenotype of Fancd2^RNAi.UAS, Scer\GAL4^ey.PU

(Marek et al., 2008)

Other

Statement

Reference

Mnn1^e200, Scer\GAL4^ey.PU, wts^x1/wts[+] has eye | chemical conditional phenotype

(Marek et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

A Mnn1^e200 mutant background enhances the rate of eye tumor formation in response to nitrogen mustard compared to wts^x1/+ mutant controls.

The presence of homozygous Mnn1^e200 does not alter the rough eye phenotype seen in flies expressing Fancd2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.PU in response to the cross-linking agent cisplatinum.

(Marek et al., 2008)

Homozygous Mnn1^e200 mutants (Df(2L)Mnn1^e200 mutants in which milt function is rescued by expression of the milt⁺²² transgene) that have a wts^x1/+ background show a two-fold greater number of tumorigenic foci than wts^x1/+ single mutants. After treatment with ionizing radiation, the number of tumorigenic foci is two to three times higher in these mutants than in controls and is 7-fold higher after treatment with nitrogen mustard.

The viability of Mnn1^e200 mutants (Df(2L)Mnn1^e200 mutants in which milt function is rescued by expression of the milt⁺²² transgene) is not affected by a sn^w background.

(Busygina et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mnn1^e200

(Marek et al., 2008, Busygina et al., 2004)

mnn1^e200

(Cerrato et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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