ProtA is expressed from its own promoter and is tagged with EGFP in frame with the last ProtA exon. ProtA sequences correspond to 280bp upstream of the predicted 5' UTR to the last exon.
ProtAEGFP rescues Df(2L)FDD-0338164
ProtAEGFP partially rescues Df(2L)ΔMst35B
ProtAT:Avic\GFP-EGFP rescues the abnormal nuclear morphology of spermatids which is seen in Df(2L)ΔMst35B males. However, a fraction of spermatids are stil eliminated in the rescued animals.
One copy of Mst35BaT:Avic\GFP-EGFP reduces the number of malformed sperm heads seen in Df(2L)FDD-0338164 males, while two copies of Mst35BbT:Avic\GFP-EGFP completely rescue this phenotype.