Allele Dmel\Mst35Bb^{T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\Mst35Bb^{T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0197722
Feature type	allele	Associated gene	Dmel\Mst35Bb

Allele class
Mutagen	in vitro construct - coding region fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Raja and Renkawitz-Pohl, 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Mst35Bb is expressed from its own promoter and is tagged with T:Avic\GFP-EGFP in frame with the last Mst35Bb exon. Mst35Bb sequences correspond to 665bp upstream of the predicted 5' UTR to the last exon. (Raja and Renkawitz-Pohl, 2005)
Carried in construct	P{protamineB-eGFP} (Rathke et al., 2007, Raja and Renkawitz-Pohl, 2005, Konev et al., 2007, Raja and Renkawitz-Pohl, 2006, Vardanyan et al., 2008, Rathke et al., 2010, Yang et al., 2011, Yang and Lu, 2011, Bonnefoy et al., 2007, Leser et al., 2012)
Tagged with	T:Avic\GFP^EGFP (Raja and Renkawitz-Pohl, 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference Mst35Bb^{T:Avic\GFP-EGFP} is a non-suppressor of male sterile phenotype of Df(3L)ri-79c/Mst77F¹ (Raja and Renkawitz-Pohl, 2005)
Phenotype Manifest In
NOT Suppressor of
Statement Reference Mst35Bb^{T:Avic\GFP-EGFP} is a non-suppressor of nucleus & late spermatid phenotype of Df(3L)ri-79c/Mst77F¹ (Raja and Renkawitz-Pohl, 2005)
Additional Comments
Genetic Interactions
Statement Reference Expression of Mst35Bb^{T:Avic\GFP-EGFP} fails to suppress the spermatid phenotype of Mst77F¹/Df(3L)ri-79c males. (Raja and Renkawitz-Pohl, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Mst35Bb^{T:Avic\GFP-EGFP} rescues Df(2L)FDD-0338164 (Rathke et al., 2010)
Comments	One copy of Mst35Bb[T:Avic\GFP-EGFP] reduces the number of malformed sperm heads seen in Df(2L)FDD-0338164 males, while two copies of Mst35Bb[T:Avic\GFP-EGFP] completely rescue this phenotype. (Rathke et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Mst35Bb^{T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Leser et al., 2012, Biol. Open 1(6): 597--606 The bromodomain-containing protein tBRD-1 is specifically expressed in spermatocytes and is essential for male fertility. [FBrf0220181] Yang et al., 2011, Mol. Biol. Cell 22(7): 976--987 Regulation of flagellar motility by the conserved flagellar protein CG34110/Ccdc135/FAP50. [FBrf0214073] Yang and Lu, 2011, Biol. Reprod. 84(5): 1005--1015 Drosophila sperm motility in the reproductive tract. [FBrf0214387] Rathke et al., 2010, Europ. J. Cell Biol. 89(4): 326--338 Distinct functions of Mst77F and protamines in nuclear shaping and chromatin condensation during Drosophila spermiogenesis. [FBrf0210142] Vardanyan et al., 2008, BMC Dev. Biol. 8: 68 Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in Drosophila. [FBrf0207335] Bonnefoy et al., 2007, PLoS Genet. 3(10): e182 The essential role of Drosophila HIRA for de novo assembly of paternal chromatin at fertilization. [FBrf0200564] Konev et al., 2007, Science 317(5841): 1087--1090 CHD1 motor protein is required for deposition of histone variant H3.3 into chromatin in vivo. [FBrf0201971] Rathke et al., 2007, J. Cell Sci. 120(9): 1689--1700 Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila. [FBrf0200503] Raja and Renkawitz-Pohl, 2005, Mol. Cell. Biol. 25(14): 6165--6177 Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus. [FBrf0188104]
Erratum	Raja and Renkawitz-Pohl, 2006, Mol. Cell. Biol. 26(9): 3682 Author's Correction. Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus. [FBrf0195018]

Allele Dmel\Mst35BbT:Avic\GFP-EGFP

Allele Dmel\Mst35Bb^{T:Avic\GFP-EGFP}