spindle & neuroblast & larva
The increased number of brain neuroblast (NB) cells characteristic for aurA8839 mutant third instar larvae is slightly elevated further by combination with homozygous mad2G6595, whereas the increased mitotic index, the highly condensed chromosome appearance in mitotic cells and the tumorigenic potential of the double mutant tissue in an allograft assay are not significantly affected compared to aurA8839 single mutant. The delay in the timing of mitotic cell cycle events is ameliorated in aurA8839;mad2G6595 brain NBs compared to aurA8839 cells but not wild-type level, no lagging chromatids (indicative of incorrect spindle attachment) are observed during anaphase and telophase.
aurA8839;Sas-4s2214;mad2G6595 triple mutants show high rate of ploidy defects in third instar larval brains, the number of neuroblasts or the mitotic cells is highly increased relative to wild-type but significantly different from aurA8839;Sas-4s2214 double mutants.
The increased neuroblast number and mitotic index seen in aurA8839/aurA17961 transheterozygote larvae is not enhanced by combination with BubR1KEN.T:Disc\RFP-mRFP,BubR1k06109 (the mitotic index in the double mutants is even slightly decreased), whereas the rate of ploidy defects is increased compared to either of the single gene mutants.