FlyBase Allele Report: Dmel\aurA[8839]

General Information

Symbol

Dmel\aurA⁸⁸³⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0197863

Feature type

allele

Associated gene

Dmel\aurA

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Wang et al., 2006)

Progenitor genotype

Cytology

Description

Amino acid replacement: K377term.

(Lee et al., 2006, Wang et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:11,964,546..11,964,546 [+]

Nucleotide change:

A11964546T

Amino acid change:

K377term | aurA-PA

Reported amino acid change:

K377term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lee et al., 2006, Wang et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of brain cancer

CEA

(Rossi et al., 2018, Wang et al., 2006)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of brain cancer

is ameliorated by N^l1N-ts1

(Wang et al., 2006)

is ameliorated by dap^UAS.cLa

(Wang et al., 2006)

is ameliorated by numb^PTB.UAS.GFP

(Wang et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal endomitotic cell cycle

(Unhavaithaya and Orr-Weaver, 2012)

abnormal mitotic cell cycle | third instar larval stage

(Gallaud et al., 2022, Caous et al., 2015)

abnormal mitotic cell cycle | third instar larval stage (with aurA¹⁷⁹⁶¹)

(Caous et al., 2015)

abnormal neuroanatomy

(Lee et al., 2006, Wang et al., 2006)

abnormal neuroanatomy | third instar larval stage

(Gallaud et al., 2022)

decreased occurrence of cell division | third instar larval stage

(Gallaud et al., 2022)

increased cell number | third instar larval stage

(Gallaud et al., 2022)

lethal - all die before end of pupal stage

(Lee et al., 2006)

lethal - all die before end of pupal stage | recessive

(Wang et al., 2006)

neoplasia | third instar larval stage

(Gallaud et al., 2022, Caous et al., 2015)

some die during pupal stage

(Lee et al., 2006)

some die during pupal stage | recessive

(Wang et al., 2006)

Phenotype Manifest In

chromosome | third instar larval stage

(Caous et al., 2015)

larval brain

(Wang et al., 2006)

larval brain | third instar larval stage

(Gallaud et al., 2022, Caous et al., 2015, Unhavaithaya and Orr-Weaver, 2012)

larval brain | third instar larval stage (with aurA¹⁷⁹⁶¹)

(Caous et al., 2015)

larval central nervous system

(Lee et al., 2006)

larval neuroblast | increased number | third instar larval stage

(Gallaud et al., 2022, Caous et al., 2015)

larval neuroblast | increased number | third instar larval stage (with aurA¹⁷⁹⁶¹)

(Caous et al., 2015)

larval neuroblast | third instar larval stage

(Gallaud et al., 2022)

neuroblast | supernumerary & larval brain

(Lee et al., 2006, Wang et al., 2006)

spindle & neuroblast & larva

(Lee et al., 2006)

subperineurial glial cell

(Unhavaithaya and Orr-Weaver, 2012)

Detailed Description

Statement

Reference

aurA⁸⁸³⁹ third instar larval brains show low but significant levels of aneuploidy/polyploidy. Their neuroblasts spend longer in mitosis. Brain material transplanted into wild-type host abdomens develops into tumors.

(Gallaud et al., 2022)

aurA⁸⁸³⁹ homozygous larvae display a marked brain overgrowth with highly increased number of neuroblasts and mitotic index, increased rate of aneu- or polyploid cells and highly condensed chromosomes in mitotic cells due to significantly prolonged M phase relative to controls. However, no lagging chromatids (indicative of incorrect spindle attachment) are observed during anaphase and telophase. The mutant brain tissue has the ability to efficiently induce tumor formation in an tissue allograft assay. The neuroblast number, the mitotic index and rate of ploidy defects is increased also in brains from aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ transheterozygotes.

(Caous et al., 2015)

Homozygous aur⁸⁸³⁹ mutants exhibit increased brain volume at the wandering third instar larval stage. The increased size of the brain lobes in this mutant is associated with increased subperineurial glia ploidy. Both the ploidy of mononucleate subperineurial glia and the number of nuclei in multinucleate subperineurial glial cell increases. The increased ploidy results in a functional blood-brain barrier despite the large neuronal mass. Only a small fraction of the aur⁸⁸³⁹ mutant brains display dye permeability.

aur⁸⁸³⁹ mutants do not exhibit polyploidy in endocyclic larval salivary gland cells.

(Unhavaithaya and Orr-Weaver, 2012)

Mutants show a massive increase in larval brain neuroblasts, developing more than 1000 central brain neuroblasts at stages where wild-type larvae have only 95-100 neuroblasts. The central nervous system is expanded compared to wild type in the mutant larvae. The orientation of the mitotic spindle is essentially randomised relative to the apical/basal cortical polarity axis in mutant metaphase neuroblasts, in contrast to wild-type metaphase neuroblasts where the mitotic spindle is aligned within 15⁰ of the center of the apical/basal cortical polarity axis. Mutant neuroblasts also have defects in telophase spindle orientation, with approximately 15% of the neuroblasts dividing symmetrically.

(Lee et al., 2006)

Homozygous larval brains contain supernumerary neuroblasts. At 24 hours after larval hatching (ALH) the number of neuroblasts in the brain is similar in mutant and wild-type larvae, however, in mutant larvae the number of neuroblasts increases dramatically during larval life, to more than a thousand at 120 ALH during an extended larval life. The number of apoptotic cells in the mutant larval brain appears normal.

The brain can grow up to 10 times the wild-type size in mutant larvae.

(Wang et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, enhanceable by aurB¹⁶⁸⁹/aurB^35.33

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, enhanceable by aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by mad2^G6595

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has neoplasia | third instar larval stage phenotype, non-enhanceable by mad2^G6595/mad2^G6595

(Caous et al., 2015)

aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ has neoplasia | third instar larval stage phenotype, non-enhanceable by BubR1^k06109/BubR1^KEN.mRFP1

(Caous et al., 2015)

Suppressed by

Statement

Reference

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, suppressible by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has increased cell number | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has neoplasia | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, suppressible by mad2^G6595/mad2^G6595

(Caous et al., 2015)

aurA⁸⁸³⁹ has abnormal neuroanatomy phenotype, suppressible by dap^UAS.cLa/Scer\GAL4^wor.PA

(Wang et al., 2006)

aurA⁸⁸³⁹ has abnormal neuroanatomy phenotype, suppressible by aPKC^unspecified

(Wang et al., 2006)

aurA⁸⁸³⁹ has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^wor.PA/numb^PTB.UAS.GFP

(Wang et al., 2006)

aurA⁸⁸³⁹ has abnormal neuroanatomy phenotype, suppressible by N^l1N-ts1

(Wang et al., 2006)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal mitotic cell cycle | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by mad2^G6595

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has decreased occurrence of cell division | third instar larval stage phenotype, non-suppressible by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has increased cell number | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has increased cell number | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has neoplasia | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

Other

Statement

Reference

Sas-4^s2214, aurA⁸⁸³⁹, mad2^G6595 has abnormal mitotic cell cycle | third instar larval stage phenotype

(Caous et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, enhanceable by aurB¹⁶⁸⁹/aurB^35.33

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, enhanceable by aurB¹⁶⁸⁹/aurB^2A43

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage | increased number phenotype, enhanceable by mad2^G6595/mad2^G6595

(Caous et al., 2015)

NOT Enhanced by

Statement

Reference

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage | increased number phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, non-enhanceable by mad2^G6595

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, non-enhanceable by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-enhanceable by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has FlyBase_internalproperty type:chromosome:chromosome | third instar larval stage phenotype, non-enhanceable by mad2^G6595/mad2^G6595

(Caous et al., 2015)

aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ has larval neuroblast | third instar larval stage | increased number phenotype, non-enhanceable by BubR1^k06109/BubR1^KEN.mRFP1

(Caous et al., 2015)

aurA⁸⁸³⁹, mad2^G6595 has larval neuroblast | third instar larval stage | increased number phenotype, non-enhanceable by Sas-4^s2214/Sas-4^s2214

(Caous et al., 2015)

Suppressed by

Statement

Reference

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, suppressible by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | increased number | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval brain | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has neuroblast | supernumerary & larval brain phenotype, suppressible by dap^UAS.cLa/Scer\GAL4^wor.PA

(Wang et al., 2006)

aurA⁸⁸³⁹ has neuroblast | supernumerary & larval brain phenotype, suppressible by aPKC^unspecified

(Wang et al., 2006)

aurA⁸⁸³⁹ has neuroblast | supernumerary & larval brain phenotype, suppressible by Scer\GAL4^wor.PA/numb^PTB.UAS.GFP

(Wang et al., 2006)

aurA⁸⁸³⁹ has neuroblast | supernumerary & larval brain phenotype, suppressible by N^l1N-ts1

(Wang et al., 2006)

NOT suppressed by

Statement

Reference

aurA⁸⁸³⁹, aurB¹⁶⁸⁹/aurB^2A43 has larval neuroblast | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182D.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, non-suppressible by mad2^G6595

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | third instar larval stage phenotype, non-suppressible by mad2^G6595/Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | increased number | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval neuroblast | increased number | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^{T182A.UASp.RR.GFP}

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has larval brain | third instar larval stage phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/polo^UASp.RR.GFP

(Gallaud et al., 2022)

aurA⁸⁸³⁹ has spindle & neuroblast & larva phenotype, non-suppressible by Scer\GAL4^wor.PA/numb^UAS.cWa

(Lee et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of brain neuroblast (NB) cells characteristic for aurA⁸⁸³⁹ mutant third instar larvae is slightly elevated further by combination with homozygous mad2^G6595, whereas the increased mitotic index, the highly condensed chromosome appearance in mitotic cells and the tumorigenic potential of the double mutant tissue in an allograft assay are not significantly affected compared to aurA⁸⁸³⁹ single mutant. The delay in the timing of mitotic cell cycle events is ameliorated in aurA⁸⁸³⁹;mad2^G6595 brain NBs compared to aurA⁸⁸³⁹ cells but not wild-type level, no lagging chromatids (indicative of incorrect spindle attachment) are observed during anaphase and telophase.

aurA⁸⁸³⁹;Sas-4^s2214;mad2^G6595 triple mutants show high rate of ploidy defects in third instar larval brains, the number of neuroblasts or the mitotic cells is highly increased relative to wild-type but significantly different from aurA⁸⁸³⁹;Sas-4^s2214 double mutants.

The increased neuroblast number and mitotic index seen in aurA⁸⁸³⁹/aurA¹⁷⁹⁶¹ transheterozygote larvae is not enhanced by combination with BubR1^{KEN.T:Disc\RFP-mRFP},BubR1^k06109 (the mitotic index in the double mutants is even slightly decreased), whereas the rate of ploidy defects is increased compared to either of the single gene mutants.

(Caous et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aurA⁸⁸³⁹ is rescued by Scer\GAL4^wor.PA/aurA^{UAS.GFP(S65T)}

(Wang et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

aur⁸⁸³⁹

(Wang et al., 2006)

aurA⁸⁸³⁹

(Gallaud et al., 2022, Rossi et al., 2018, Caous et al., 2015, Unhavaithaya and Orr-Weaver, 2012, Sousa-Nunes et al., 2009, Wang et al., 2009, Lee et al., 2006)

l(3)LL-8839

(Lee et al., 2006)

l(3)LL8839

(Wang et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

Open Close