Allele Dmel\MtnB^ΔATG

General Information
Symbol	Dmel\MtnB^ΔATG	Species	D. melanogaster
Name		FlyBase ID	FBal0197948
Feature type	allele	Associated gene	Dmel\MtnB

Allele class
Mutagen	FLPase, recombination, SCEI endonuclease, I-CreI endonuclease, gene targeting by homologous recombination
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	FLPase (Egli et al., 2006) gene targeting by homologous recombination (Egli et al., 2006) I-CreI endonuclease (Egli et al., 2006) recombination (Egli et al., 2006) SCEI endonuclease (Egli et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	MtnB^{ΔATG.Scer\SceI.RS} (Egli et al., 2006)
Nature of the lesion	Statement Reference Targeted recombination event at the MtnB locus, using P{(FRT.w^3xP3.MtnB^{ΔATG.Scer\SceI.RS})} as the donor template. The resulting duplication of the target MtnB gene has been reduced to a single copy using the Crei\I-CreI enzyme. The resulting allele has the ATG start codon and several neighbouring amino acids replaced by a NotI site. (Egli et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of partially lethal - majority die phenotype of MTF-1^{Δ580-791.tub} (Günther et al., 2012, Günther et al., 2012) MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of partially lethal - majority die phenotype of MTF-1^{Δ637-791.tub} (Günther et al., 2012, Günther et al., 2012) MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of visible phenotype of MTF-1^{Δ580-791.tub} (Günther et al., 2012, Günther et al., 2012) MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of visible phenotype of MTF-1^{Δ637-791.tub} (Günther et al., 2012, Günther et al., 2012)
Other
Statement Reference MtnA^ΔATG, MtnB^ΔATG, MtnC^ΔATG, MtnD^OreR has chemical sensitive phenotype (Egli et al., 2006, Egli et al., 2006) MtnA^ΔATG, MtnB^ΔATG, MtnC^ΔATG, MtnD^ΔATG has chemical sensitive phenotype (Egli et al., 2006) MtnA^ΔATG, MtnB^ΔATG, MtnD^OreR has chemical sensitive phenotype (Egli et al., 2006) MtnB^ΔATG, MtnD^OreR has chemical sensitive phenotype (Egli et al., 2006, Egli et al., 2006)
Phenotype Manifest In
Enhancer of
Statement Reference MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of wing phenotype of MTF-1^{Δ580-791.tub} (Günther et al., 2012, Günther et al., 2012) MtnD^OreR, MtnD^OreR, MtnC^ΔATG, MtnC^ΔATG, MtnB^ΔATG, MtnB^ΔATG, MtnA^ΔATG, MtnA^ΔATG is an enhancer of wing phenotype of MTF-1^{Δ637-791.tub} (Günther et al., 2012, Günther et al., 2012)
Additional Comments
Genetic Interactions
Statement Reference The wing defects seen in flies expressing MTF-1[Δ580-791.tub] or MTF-1[Δ761-791.tub] are enhanced by the "qMtn" mutant combination (MtnA[ΔATG] MtnB[ΔATG] MtnC[ΔATG] MtnD[OreR]) and the mutant flies show a very low eclosion rate. (Günther et al., 2012) MtnA^ΔATG MtnB^ΔATG MtnC^ΔATG MtnD^OreR quadruple mutants are viable and fertile, but are highly sensitive to copper and cadmium load in food, showing no survival on 0.5mM copper or 50μM cadmium concentrations. The quadruple mutants are not sensitive to copper depletion. MtnB^ΔATG MtnD^OreR double mutants show greatly reduced survival on 50μM cadmium concentrations compared to controls. MtnA^ΔATG MtnB^ΔATG MtnD^OreR triple mutants show greatly reduced survival on 0.5mM copper or 50μM cadmium concentrations compared to controls. With normal food, the life span of MtnA^ΔATG MtnB^ΔATG MtnC^ΔATG MtnD^OreR quadruple homozygotes is similar to that of controls. However, at 50μM copper, the mean life span of quadruple homozygous males is 6 days shorter than that of controls, and at 75μM copper, the mean life span of quadruple homozygous males is 14 days shorter than that of controls. The mean life span of quadruple homozygous females is 3 days shorter than that of controls at both 50μM and 75μM copper. (Egli et al., 2006) MtnB^ΔATG MtnD^OreR double mutants show reduced survival on 100mM cadmium concentrations in food compared to controls. MtnA^ΔATG MtnB^ΔATG MtnC^ΔATG MtnD^OreR quadruple mutants show little or no survival on 1mM copper or 100mM cadmium concentrations in food compared to controls. MtnA^ΔATG MtnB^ΔATG MtnC^ΔATG MtnD^ΔATG quadruple mutants show little or no survival on 1mM copper or 100mM cadmium concentrations in food compared to controls. (Egli et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	MtnB^- (Egli et al., 2006) MtnB^ΔATG (Egli et al., 2006, Egli et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Günther et al., 2012, Int. J. Biochem. & Cell Biol. 44(2): 404--411 Dissection of Drosophila MTF-1 reveals a domain for differential target gene activation upon copper overload vs. copper starvation. [FBrf0217154] Egli et al., 2006, Mol. Cell. Biol. 26(6): 2286--2296 A family knockout of all four Drosophila metallothioneins reveals a central role in copper homeostasis and detoxification. [FBrf0191190] Egli et al., 2006, Genes Cells 11(6): 647--658 The four members of the Drosophila metallothionein family exhibit distinct yet overlapping roles in heavy metal homeostasis and detoxification. [FBrf0194402]

Allele Dmel\MtnBΔATG

Allele Dmel\MtnB^ΔATG