Allele Dmel\Tip60^{dsRNA.Z.Scer\UAS}

General Information
Symbol	Dmel\Tip60^{dsRNA.Z.Scer\UAS}	Species	D. melanogaster
Name		FlyBase ID	FBal0198012
Feature type	allele	Associated gene	Dmel\Tip60

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Zhu et al., 2007) in vitro construct - RNAi (Zhu et al., 2007)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat of 613bp of Tip60 sequence (nucleotides 871-1483) in sense-antisense orientation. The repeat sequences are separated by an ATCTA polylinker. (Zhu et al., 2007)
Carried in construct	P{UAS-Tip60.RNAi.Z} (Zhu et al., 2007, Sarthi and Elefant, 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^337Y (Zhu et al., 2007) lethal \| partially, with Scer\GAL4^how-24B (Zhu et al., 2007) lethal \| pupal stage, with Scer\GAL4^Act5C.PI (Zhu et al., 2007) lethal \| pupal stage, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) lethal \| pupal stage, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) neuroanatomy defective \| larval stage, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) neuroanatomy defective \| larval stage, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011)
Phenotype Manifest In
	terminal bouton, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) terminal bouton, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) type I bouton \| ectopic, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Scer\GAL4^elav-C155 (Sarthi and Elefant, 2011) type Ib terminal bouton \| ectopic, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011) type Is terminal bouton, with Scer\GAL4^Mef2.247 (Sarthi and Elefant, 2011)
Detailed Description
	Statement Reference Pre-synaptic expression of Tip60[dsRNA.Z.Scer\UAS] under the control of Scer\GAL4[elav-C155] results in pupal lethality.There is a significant increase in the total number of synaptic boutons in Tip60[E431Q.Scer\UAS]-expressing larvae, when compared to wild-type controls. There is a substantially larger expansion of type-I boutons compared to type-Ib in these mutants. There is also a slight increase in the number of satellite boutons in these mutants compared to controls. This increase in bouton number is accompanied by an excess of futsch-associated microtubule looping and splaying. Post-synaptic expression of Tip60[dsRNA.Z.Scer\UAS] under the control of Scer\GAL4[Mef2.247] results in pupal lethality. Mutant larvae exhibit a significant reduction in bouton number, specifically in 1s boutons, as well as the absence of satellite boutons. (Sarthi and Elefant, 2011) Expression of Tip60^{dsRNA.Z.Scer\UAS} under the control of Scer\GAL4^how-24B results in a reduction in viability. The flies die at a broad range of developmental stages, depending on the insertion line used, beginning from early pupae to directly before eclosion. The apparent cause of lethality later in development appears to be due to an inability to eclose from the pupal casing. (Zhu et al., 2007)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Tip60 gene. (Zhu et al., 2007)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Tip60^{dsRNA.Z.Scer\UAS}
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Sarthi and Elefant, 2011, PLoS ONE 6(10): e26202 dTip60 HAT Activity Controls Synaptic Bouton Expansion at the Drosophila Neuromuscular Junction. [FBrf0216592] Zhu et al., 2007, Genetics 175(3): 1229--1240 The cloning and characterization of the histone acetyltransferase human homolog Dmel\TIP60 in Drosophila melanogaster: Dmel\TIP60 is essential for multicellular development. [FBrf0200176]

Allele Dmel\Tip60dsRNA.Z.Scer\UAS

Allele Dmel\Tip60^{dsRNA.Z.Scer\UAS}