Open Close
General Information
Symbol
Dmel\Mnn1dsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0198070
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of exon 2 of the Mnn1 gene, followed by intron 2, followed by exon 2 in reverse order.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of Mnn1dsRNA.Scer\UAS following a 1 hour heat shock at 37oC, under the control of Scer\GAL4hs.PB, Scer\GAL4nos.PG, Scer\GAL4arm.PS or Scer\GAL4αTub84B.PL show embryonic lethality. The lethality follows a dramatic increase in cell death. However, expression of Mnn1dsRNA.Scer\UAS under the control of Scer\GAL4nos.PG, Scer\GAL4αTub84B.PL or Scer\GAL4arm.PS at 25oC does not result in this lethality. Expression of Mnn1dsRNA.Scer\UAS under the control of Scer\GAL4nos.PG following 2 hours in hypoxic conditions (5% O2) results in embryonic lethality, while expression at 21% O2 results in similar survival levels to wild-type flies. Expression of Mnn1dsRNA.Scer\UAS under the control of Scer\GAL4αTub84B.PL in a hyper-osmolarity environment (0.2M NaCl) results in leads to lethality, while flies of the same genotype raised in a 0M NaCl environment show similar survival levels to wild-type flies. Exposure of adult flies that express Mnn1dsRNA.Scer\UAS under the control of Scer\GAL4αTub84B.PL to 5mM paraquat results in results in decreased viability compared to wild-type flies exposed to this chemical. Scer\GAL4αTub84B.PL>Mnn1dsRNA.Scer\UAS expression does not alter the development of the trachea in control conditions but in a low oxygen environment, the induction of lateral branching seen in wild-type larvae is inhibited in Scer\GAL4αTub84B.PL>Mnn1dsRNA.Scer\UAS larvae.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Transcription from the P{UAS-Mnn1.RNAi} construct should produce Mnn1 dsRNA, resulting in dsRNA interference (RNAi) of the Mnn1 gene.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Mnn1dsRNA.Scer\UAS
Mnn1dsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (1)