FB2025_02 , released April 17, 2025
Allele: Dmel\Rack1EE
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General Information
Symbol
Dmel\Rack1EE
Species
D. melanogaster
Name
FlyBase ID
FBal0198123
Feature type
allele
Associated gene
Dmel\Rack1
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Kadrmas et al., 2007)
Mutagen
ethyl methanesulfonate
(Kadrmas et al., 2007)
Progenitor genotype
Cytology
Description

Amino acid replacement: S81F.

(Kadrmas et al., 2007)

Nucleotide substitution: C?T.

(Kadrmas et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:7,826,849..7,826,849 [-]
Nucleotide change:

C7826849T

Reported nucleotide change:

C?T

Amino acid change:

S81F | Rack1-PA; S81F | Rack1-PB; S81F | Rack1-PC; S81F | Rack1-PD

Reported amino acid change:

S81F

(Kadrmas et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  muscular dystrophy
      modeled by DgO86
      (Kucherenko et al., 2011)
      modeled by DysRNAi.NH2.UAS
      (Kucherenko et al., 2011)
      Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
       

      FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

      (Kucherenko et al., 2011)
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Although Rack1EE/Rack1EY00128 mutants show larval lethality, up to 35% of animals are escapers. Females at 3 days posteclosion contain ovaries that are reduced in size but have no gross morphological defects. At 7 days, the ovarioles are crowded with small, but mature, stage 14 eggs. Rack1EE/Rack1EY00128 germaria have reduced numbers of germline stem cells but these are never missing completely. The number of germ-line derived cells in the egg chambers of these mutants does not seem affected. Oogenesis can proceed relatively normally to stage 14, but the resulting eggs are not laid and accumulate in the ovariole.

      Rack1EE/Df(2L)RACK1-mts and Rack1EE/Rack11.8 escapers display a range of phenotypes including sluggish movement, difficulty in exiting the pupal case, cuticle that does not harden, and wings that are blistered, abnormally small, or that fail to unfold. Escaper females are incapable of laying eggs and the size of the ovary is reduced compared to wild type but overall body size is unaffected.

      (Kadrmas et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      Rack1[+]/Rack1EE is an enhancer of abnormal size | adult stage phenotype of sl2

      (Murillo-Maldonado et al., 2011)
      NOT Enhancer of
      Statement
      Reference

      Rack1[+]/Rack1EE is a non-enhancer of increased cell number | adult stage phenotype of sl2

      (Murillo-Maldonado et al., 2011)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Rack1[+]/Rack1EE is an enhancer of indirect flight muscle cell phenotype of DysRNAi.NH2.UAS, Scer\GAL4Act.PU

      (Kucherenko et al., 2011)

      Rack1[+]/Rack1EE is an enhancer of wing blade phenotype of sl2

      (Murillo-Maldonado et al., 2011)

      Rack1[+]/Rack1EE is an enhancer of wing vein | ectopic phenotype of sl2

      (Murillo-Maldonado et al., 2011)
      NOT Enhancer of
      Statement
      Reference

      Rack1[+]/Rack1EE is a non-enhancer of indirect flight muscle cell phenotype of DgRNAi.UAS, Scer\GAL4Act.PU

      (Kucherenko et al., 2011)

      Rack1[+]/Rack1EE is a non-enhancer of photoreceptor cell R7 | adult stage phenotype of sl2

      (Murillo-Maldonado et al., 2011)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Rack1 Dys double heterozygous flies (Rack1EE/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

      Rack1EE DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

      One copy of Rack1EE enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

      One copy of Rack1EE does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

      Rack1EE Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

      (Kucherenko et al., 2011)

      One copy of Rack1EE enhances the reduction in wing blade area seen in homozygous sl2 males.

      One copy of Rack1EE significantly enhances the ectopic wing vein phenotype seen in sl2 homozygotes.

      One copy of Rack1EE does not enhance the percentage of sl2 mutant ommatidia that contain extra R7 photoreceptors.

      (Murillo-Maldonado et al., 2011)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      R. Holmgren

      (Kadrmas et al., 2007)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RACK1EE
      (Kadrmas et al., 2007)
      Rack1EE
      (Kucherenko et al., 2011, Murillo-Maldonado et al., 2011)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)