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General Information
Symbol
Dmel\Rack1EE
Species
D. melanogaster
Name
FlyBase ID
FBal0198123
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C7826849T

Reported nucleotide change:

C?T

Amino acid change:

S81F | Rack1-PA; S81F | Rack1-PB; S81F | Rack1-PC; S81F | Rack1-PD

Reported amino acid change:

S81F

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: S81F.

Nucleotide substitution: C?T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 

FlyBase curator comment: Interactions with Dg[086] and/or Dg[323] were detected in a Dg[086]/+ or Dg[323]/+ background which exhibits no obvious changes in muscle morphology. Nonetheless, these interactions have been captured as 'modifier' ('exacerbates') annotations here to best capture the experimental finding and the authors' intention.

Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Although Rack1EE/Rack1EY00128 mutants show larval lethality, up to 35% of animals are escapers. Females at 3 days posteclosion contain ovaries that are reduced in size but have no gross morphological defects. At 7 days, the ovarioles are crowded with small, but mature, stage 14 eggs. Rack1EE/Rack1EY00128 germaria have reduced numbers of germline stem cells but these are never missing completely. The number of germ-line derived cells in the egg chambers of these mutants does not seem affected. Oogenesis can proceed relatively normally to stage 14, but the resulting eggs are not laid and accumulate in the ovariole.

Rack1EE/Df(2L)RACK1-mts and Rack1EE/Rack11.8 escapers display a range of phenotypes including sluggish movement, difficulty in exiting the pupal case, cuticle that does not harden, and wings that are blistered, abnormally small, or that fail to unfold. Escaper females are incapable of laying eggs and the size of the ovary is reduced compared to wild type but overall body size is unaffected.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

Rack1[+]/Rack1EE is an enhancer of abnormal size | adult stage phenotype of sl2

NOT Enhancer of
Statement
Reference

Rack1[+]/Rack1EE is a non-enhancer of increased cell number | adult stage phenotype of sl2

Phenotype Manifest In
Enhancer of
Statement
Reference

Rack1[+]/Rack1EE is an enhancer of wing blade phenotype of sl2

Rack1[+]/Rack1EE is an enhancer of wing vein | ectopic phenotype of sl2

NOT Enhancer of
Statement
Reference

Rack1[+]/Rack1EE is a non-enhancer of photoreceptor cell R7 | adult stage phenotype of sl2

Other
Additional Comments
Genetic Interactions
Statement
Reference

Rack1 Dys double heterozygous flies (Rack1EE/Df(3R)Exel6184) exhibit indirect flight muscle degeneration.

Rack1EE DgO86 double heterozygous flies exhibit indirect flight muscle degeneration.

One copy of Rack1EE enhances the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of Rack1EE does not enhance the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

Rack1EE Dg323 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of Rack1EE enhances the reduction in wing blade area seen in homozygous sl2 males.

One copy of Rack1EE significantly enhances the ectopic wing vein phenotype seen in sl2 homozygotes.

One copy of Rack1EE does not enhance the percentage of sl2 mutant ommatidia that contain extra R7 photoreceptors.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)