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General Information
Symbol
Dmel\NmnatUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zhai
FlyBase ID
FBal0198130
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Nmnat
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Nmnat (sequences correspond to cDNA clone AT23490).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of NmnatScer\UAS.cZa under the control of Scer\GAL4elav.PU has no effect on learning and memory in an aversive phototaxis assay at 20 days post eclosion. No locomotor activity defects are seen, as measured by climbing performance and brains do not display any morphological defects.

Overexpression of NmnatScer\UAS.cZa under the control of Scer\GAL4elav-C155 has no effect on dendrite outgrowth.

Expression of NmnatScer\UAS.cZa under the control of Scer\GAL4GMR.PF protects flies against neuronal degeneration in response to intense light exposure; eyes expressing this transgene show less vacuolization and a higher number of rhabdomeres than wild-type flies in response to 30 days of light exposure.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The anterograde mitochondria transport defects of single cell milt33-853 larval motor neuron clones are not rescued by expression of NmnatScer\UAS.cZa under the control of Scer\GAL4D42.

Expression of NmnatScer\UAS.cZa under the control of Scer\GAL4elav-C155 protects both wild-type and milt33-853 motor neuron clones from degeneration after axotomy at 16 hours after crushing.

Whereas wtsx1 ddaC MARCM clones show a simplifed dendritic arbor with significantly reduced numbers of terminal branches, wtsx1 clones overexpressing NmnatScer\UAS.cZa under the control of Scer\GAL4elav-C155 elaborate dendrites with terminal branch numbers that do not differ statistically from wild-type controls. A similar rescue is seen in class IV neurons.

Co-expression of NmnatScer\UAS.cZa suppresses the rhabdomere degeneration phenotype induced by Scer\GAL4GMR.PF>Atx-1Scer\UAS.cTa.

The loss of rhabdomere phenotype of rdgAunspecified mutants is significantly rescued by either NmnatScer\UAS.cZa or NmnatWR.Scer\UAS, under the control of Scer\GAL4GMR.PF. The size of vacuoles that are present in the retina and photoreceptors of rdgAunspecified mutants are reduced by expression of NmnatWR.Scer\UAS, but not NmnatScer\UAS.cZa.

The number of rhabdomeres per ommatidium is significantly rescued in trpP365 flies by expression of either NmnatScer\UAS.cZa or NmnatWR.Scer\UAS under the control of Scer\GAL4GMR.PF. However, the vacuolarization that occurs in trpP365 retinae and photoreceptors is not suppressed by either transgene.

Xenogenetic Interactions
Statement
Reference

Expression of NmnatScer\UAS.cZa suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPTScer\UAS.cWa under the control of Scer\GAL4elav.PU. The climbing defects are suppressed to near wild type levels. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed.

Expression of NmnatScer\UAS.cZa suppresses the learning and memory deficits seen in 20 day old flies expressing Hsap\MAPTR406W.Scer\UAS under the control of Scer\GAL4elav.PU. The climbing defects are also suppressed to near wild type levels. The brain vacuolisation seen at 20 days post eclosion is almost completely suppressed and fewer apoptotic cells are seen in the midbrain.

The rough eye and rhabdomere phenotypes of Scer\GAL4GMR.PF>Hsap\ATXN182Q.Scer\UAS are partially suppressed by co-expression of NmnatScer\UAS.cZa.

Complementation and Rescue Data
Comments

Post-synaptic expression of NmnatScer\UAS.cZa under the control of Scer\GAL421-7 is sufficient to rescue the dendritic phenotypes observed in NmnatΔ4790-2 ddaC clones, demonstrating a cell-autonomous function for Nmnat in the proper maintenance of class IV dendrites.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
NmnatScer\UAS.cZa
NmnatUAS.cZa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Zhai
Secondary FlyBase IDs
    References (10)