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General Information
Symbol
Dmel\Nmnat1
Species
D. melanogaster
Name
FlyBase ID
FBal0198132
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C24946083T

Amino acid change:

Q235term | Nmnat-PA; Q235term | Nmnat-PB; Q204term | Nmnat-PC; Q204term | Nmnat-PD

Reported amino acid change:

Q204term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q204term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nmnat1 mutant clones in sensory neurons in the adult wing undergo rapid degeneration affecting cell bodies as well as axons.

28 day old Nmnat1 mutant flies containing neuroblast clones exhibit β-axon loss in the mushroom body. γ and α'β neurons are not visible and there is some loss of αβ neurons.

Flies with eyes that are mostly homozygous for Nmnat1 (due to somatic clones induced using Scer\FRT and Scer\FLP1ey.PN) with heterozygous patches are smooth with a normal external morphology. Electroretinogram (ERG) recordings from such eyes belonging to young mutants show reduced depolarization and on/off responses. Transmission electron microscopy of lamina synapses from 1-day-old flies with these clones reveal morphological defects; the laminae are disorganized, with various numbers of photoreceptor terminals per cartridge. The terminals contain a fragmented cytoskeleton and misshapen membrane organelles. In presynaptic terminals, the T-bars are amorphous and less electron dense compared to wild type, but they are surrounded by clusters of normally sized synaptic vesicles. However, the size of the T-bar profile, measured by the width of platforms is significantly reduced compared to wild type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Selected as: a mutant with abnormal synapse structure in R1-R6 terminals and abnormal ERG responses from an "eyFLP" screen of flies with grossly normal eye morphology that phototax poorly.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)