Allele Dmel\Nmnat^Δ4790-2

General Information
Symbol	Dmel\Nmnat^Δ4790-2	Species	D. melanogaster
Name		FlyBase ID	FBal0198135
Feature type	allele	Associated gene	Dmel\Nmnat

Allele class	loss of function allele
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Zhai et al., 2006)
Mutagen	P-element activity (Zhai et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{EPgy2}EY04790 (Zhai et al., 2006)
Nature of the lesion	Statement Reference Imprecise excision of the P{EPgy2} element, resulting in deletion of the first three exons of the Nmnat gene. (Zhai et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage (Zhai et al., 2006) neuroanatomy defective (Wen et al., 2011) neurophysiology defective \| somatic clone (Zhai et al., 2006)
Phenotype Manifest In
	class I dendritic arborizing neuron \| somatic clone (Wen et al., 2011) class III dendritic arborizing neuron \| somatic clone (Wen et al., 2011) dendrite (Wen et al., 2011) dendrite \| somatic clone (Wen et al., 2011) dorsal multidendritic neuron ddaC \| somatic clone (Wen et al., 2011) motor neuron \| somatic clone (Wen et al., 2011) optic cartridge \| somatic clone (Zhai et al., 2006)
Detailed Description
	Statement Reference At 24-28hrs after egg laying, dendrites of the dorsal class IV neuron (ddaC) in homozygous Nmnat[Δ4790-2] mutants do not show any patterning defects and are largely indistinguishable from those of wild-type controls. Although heterozygosity for Nmnat[Δ4790-2] has no effect on dendritic outgrowth or branching of ddaC at 48 hours after egg laying, there is a significant reduction in the total number of terminal dendritic branches and total dendrite length at 120 hours after egg laying. At 72 hours after egg laying, Nmnat[Δ4790-2] heterozygotes tend to have fewer terminal dendrites, although the average number of terminal dendritic branches is not significantly different from wild-type controls until after 72 hours after egg laying. The axons of class IV neurons in Nmnat[Δ4790-2] heterozygotes are largely intact at 120 hours after egg laying. Axons enter the CNS appropriately and their projection patterns in the ventral nerve cord (VNC) are morphologically indistinguishable from wild-type controls. Whereas wild-type ddaC clones elaborate highly branched dendrites, Nmnat[Δ4790-2] clones show a significant reduction in the total number of terminal dendritic branches, effectively reducing dendritic field coverage. There is no evidence of severed or degenerated dendrites in Nmnat[Δ4790-2] clones as dendritic trunks and branches are largely intact. Class III neurons, that normally elaborate actin-rich protrusions along their dendrites, are largely devoid of terminal branches in Nmnat[Δ4790-2] clones. Class I neurons, which normally elaborate few terminal branches that cover a small receptive field, exhibit a minor, but statistically significant decrease in the total number of dendritic branches in Nmnat[Δ4790-2] clones. Although class IV neurons do not exhibit an axon degeneration phenotype in Nmnat[Δ4790-2] heterozygotes, approximately 96% of homozygous Nmnat[Δ4790-2] sensory neuron clones show extensive fragmentation of axons and a near complete loss of axon terminals in the ventral nerve cord. Different subtypes of motor neurons display both terminal and dendritic branching defects and extensive beading and fragmentation at 120 hours after egg laying in Nmnat[Δ4790-2] MARCM clones. The axon terminals of Nmnat[Δ4790-2] motor neuron clones are absent in the majority of neurons. Phenotypes are progressive, getting worse with age, as axons and dendrites of Nmnat[Δ4790-2] motor neuron clones are largely intact at 72 hours after egg laying. (Wen et al., 2011) Flies with eyes that contain Nmnat^Δ4790-2 clones show reduced depolarization and on/off responses in electroretinogram (ERG) recordings and show photoreceptor synaptic defects. (Zhai et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Nmnat^Δ4790-2 is a suppressor of neuroanatomy defective phenotype of wts^x1 (Wen et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference Nmnat^Δ4790-2 is a suppressor of class IV dendritic arborizing neuron phenotype of wts^x1 (Wen et al., 2011) Nmnat^Δ4790-2 is a suppressor of dendrite phenotype of wts^x1 (Wen et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference A transheterozygous combination of Nmnat[Δ4790-2] and wts[x1] does not show any synthetic phenotypes in affecting axon or dendrite development of class IV neurons. (Wen et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Nmnat¹ (Zhai et al., 2006) Nmnat² (Zhai et al., 2006)
Rescued by	Nmnat^Δ4790-2 is rescued by Scer\GAL4^21-7/Nmnat^WR.Scer\UAS (Wen et al., 2011)
Comments	Post-synaptic expression of the enzymatically-inactive Nmnat[WR.Scer\UAS] transgene, under the control of Scer\GAL4[21-7] is sufficient to rescue the dendritic phenotypes observed in Nmnat[Δ4790-2] ddaC clones, demonstrating a cell-autonomous function for Nmnat in the proper maintenance of class IV dendrites. Post-synaptic expression of Nmnat[Scer\UAS.cZa] under the control of Scer\GAL4[21-7] is sufficient to rescue the dendritic phenotypes observed in Nmnat[Δ4790-2] ddaC clones, demonstrating a cell-autonomous function for Nmnat in the proper maintenance of class IV dendrites. (Wen et al., 2011)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	Nmnat^Δ4790-2 Nmnat^Δ4792 (Wen et al., 2011) Δ4790-2 (Zhai et al., 2006)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Wen et al., 2011, Mol. Cell. Neurosci. 48(1): 1--8 Nmnat exerts neuroprotective effects in dendrites and axons. [FBrf0214616] Zhai et al., 2006, PLoS Biol. 4(12): e416 Drosophila NMNAT maintains neural integrity independent of its NAD synthesis activity. [FBrf0192124]

Allele Dmel\NmnatΔ4790-2

Allele Dmel\Nmnat^Δ4790-2