FB2020_03, released Jun 16, 2020

Allele: Dmel\Mnn1UAS.cCa

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General Information
Symbol
Dmel\Mnn1UAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct of a of Cerrato
FlyBase ID
FBal0198140
Feature type
allele
Associated gene
Dmel\Mnn1
Associated Insertion(s)
Carried in Construct
P{UAS-Mnn1.C}
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Cerrato et al., 2006)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-Mnn1.C}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt sequences drive expression of Mnn1.

      (Cerrato et al., 2006)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Mnn1
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class

      lethal - all die before end of P-stage, with Scer\GAL469B

      (Cerrato et al., 2006)

      lethal - all die before end of P-stage, with Scer\GAL4da.G32

      (Cerrato et al., 2006)

      lethal - all die before end of P-stage, with Scer\GAL4elav-C155

      (Cerrato et al., 2006)

      lethal - all die before end of P-stage, with Scer\GAL4how-24B

      (Cerrato et al., 2006)

      lethal - all die before end of P-stage, with Scer\GAL4RapGAP1-OK6

      (Cerrato et al., 2006)

      melanotic mass phenotype, with Scer\GAL469B

      (Cerrato et al., 2006)

      melanotic mass phenotype, with Scer\GAL4da.G32

      (Cerrato et al., 2006)

      melanotic mass phenotype, with Scer\GAL4elav-C155

      (Cerrato et al., 2006)

      melanotic mass phenotype, with Scer\GAL4how-24B

      (Cerrato et al., 2006)

      melanotic mass phenotype, with Scer\GAL4RapGAP1-OK6

      (Cerrato et al., 2006)

      short lived, with Scer\GAL469B

      (Cerrato et al., 2006)

      some die during pharate adult stage, with Scer\GAL469B

      (Cerrato et al., 2006)

      some die during pharate adult stage, with Scer\GAL4da.G32

      (Cerrato et al., 2006)

      some die during pharate adult stage, with Scer\GAL4elav-C155

      (Cerrato et al., 2006)

      some die during pharate adult stage, with Scer\GAL4how-24B

      (Cerrato et al., 2006)

      some die during pharate adult stage, with Scer\GAL4RapGAP1-OK6

      (Cerrato et al., 2006)

      viable, with Scer\GAL448Y

      (Cerrato et al., 2006)

      viable, with Scer\GAL4AB1

      (Cerrato et al., 2006)

      viable, with Scer\GAL4C1003

      (Cerrato et al., 2006)

      viable, with Scer\GAL4da.G32

      (Cerrato et al., 2006)

      viable, with Scer\GAL4dimm-929

      (Cerrato et al., 2006)

      viable, with Scer\GAL4dpp.blk1

      (Cerrato et al., 2006)

      viable, with Scer\GAL4ey.PH

      (Cerrato et al., 2006)

      viable, with Scer\GAL4GMR.PF

      (Cerrato et al., 2006)

      viable, with Scer\GAL4pnr-MD237

      (Cerrato et al., 2006)

      viable, with Scer\GAL4twi.PG

      (Cerrato et al., 2006)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies that express Mnn1Scer\UAS.cCa under the control of Scer\GAL4how-24B, Scer\GAL4elav-C155, Scer\GAL4da.G32, Scer\GAL469B, or Scer\GAL4OK6 die at stage P14 and show deletion of distal elements of the proboscis and a melanotic mass at that location. The melanotic mass is visible as early as stage P7.

      About 5% of flies expressing Mnn1Scer\UAS.cCa under the control of Scer\GAL4how-24B survive to eclose and about 25% of those expressing Mnn1Scer\UAS.cCa under the control of Scer\GAL469B. These eclosed files show a melanotic mass at the anterior proboscis, failed wing inflation and die after 2-3 days.

      Flies that express Mnn1Scer\UAS.cCa under the control of Scer\GAL469B occasionally show a cleft thorax phenotype. 100% of flies that express Mnn1Scer\UAS.cCa under the control of Scer\GAL4pnr-MD237 show a dorsal cleft along the entire thorax with disrupted chaetae orientation. The expressivity of this phenotype increases with the number of copies of Mnn1Scer\UAS.cCa that are expressed.

      Expression of Mnn1Scer\UAS.cCa under the control of Scer\GAL4ey.PH does not cause an eye phenotype.

      (Cerrato et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of Mnn1Scer\UAS.cCa under the control of Scer\GAL4pnr-MD237 in a Jra1/+ background results in an enhanced thoracic cleft compared to Mnn1Scer\UAS.cCa expression in a wild-type background.

      The cleft thorax phenotype of flies that express either Mnn1Scer\UAS.cCa or JrabZIP.Scer\UAS under the control of Scer\GAL4pnr-MD237 is enhanced when Mnn1Scer\UAS.cCa and JrabZIP.Scer\UAS are expressed together by Scer\GAL4pnr-MD237. Further, flies that coexpress Mnn1Scer\UAS.cCa and JrabZIP.Scer\UAS under the control of Scer\GAL4how-24B are lethal at pupal stage P7, which is earlier than the pharate adult lethality of Scer\GAL4how-24B>Mnn1Scer\UAS.cCa flies.

      Coexpression of Mnn1Scer\UAS.cCa and JraAsp.B.Scer\UAS under the control of Scer\GAL4pnr-MD237 results in 1-5% of flies escaping the JraAsp.B.Scer\UAS lethality to eclose. Viable flies have a severe cleft thorax. Likewise, the prepupal lethality of Scer\GAL4ey.PH>JraAsp.B.Scer\UAS flies is partially suppressed when Mnn1Scer\UAS.cCa is coexpressed.

      A kay1/+ background has no effect on the Scer\GAL4pnr-MD237>Mnn1Scer\UAS.cCa phenotype.

      Coexpression of kayScer\UAS.cEa and Mnn1Scer\UAS.cCa under the control of Scer\GAL4pnr-MD237 results in an enhancement of the cleft thorax phenotypes seen when either is expressed alone.

      Coexpression of Mnn1Scer\UAS.cCa with kaybZip.Scer\UAS, under the control of Scer\GAL4ey.PH, results in complete suppression of the Scer\GAL4ey.PH>kaybZip.Scer\UAS rough eye phenotype.

      Coexpression of Mnn1Scer\UAS.cCa and JraScer\UAS.cEa under the control of either Scer\GAL4pnr-MD237, Scer\GAL4ey.PH or Scer\GAL4how-24B results in the same phenotypes as expression of Mnn1Scer\UAS.cCa alone.

      (Cerrato et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Mnn1Scer\UAS.cCa
      Mnn1UAS.cCa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct of a of Cerrato
      Secondary FlyBase IDs
        References (2)