FlyBase Allele Report: Dmel\Mnn1[UAS.cCa]

Flies that express Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^how-24B, Scer\GAL4^elav-C155, Scer\GAL4^da.G32, Scer\GAL4^69B, or Scer\GAL4^OK6 die at stage P14 and show deletion of distal elements of the proboscis and a melanotic mass at that location. The melanotic mass is visible as early as stage P7.

About 5% of flies expressing Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^how-24B survive to eclose and about 25% of those expressing Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^69B. These eclosed files show a melanotic mass at the anterior proboscis, failed wing inflation and die after 2-3 days.

Flies that express Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^69B occasionally show a cleft thorax phenotype. 100% of flies that express Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^pnr-MD237 show a dorsal cleft along the entire thorax with disrupted chaetae orientation. The expressivity of this phenotype increases with the number of copies of Mnn1^Scer\UAS.cCa that are expressed.

Expression of Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^ey.PH does not cause an eye phenotype.

(Cerrato et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^how-24B has lethal | pharate adult stage P14 phenotype, non-enhanceable by Jra^UAS.cEa, Scer\GAL4^how-24B

(Cerrato et al., 2006)

NOT suppressed by

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^how-24B has lethal | pharate adult stage P14 phenotype, non-suppressible by Jra^UAS.cEa, Scer\GAL4^how-24B

(Cerrato et al., 2006)

Suppressor of

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 is a suppressor | partially of lethal | embryonic stage phenotype of Jra^Asp.B.UAS, Scer\GAL4^pnr-MD237

(Cerrato et al., 2006)

Mnn1^UAS.cCa, Scer\GAL4^ey.PH is a suppressor | partially of lethal | prepupal stage phenotype of Jra^Asp.B.UAS, Scer\GAL4^ey.PH

(Cerrato et al., 2006)

Other

Statement

Reference

Jra^Jbz.UAS, Mnn1^UAS.cCa, Scer\GAL4^how-24B has lethal | pupal stage P7 phenotype

(Cerrato et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by Jra¹/Jra[+]

(Cerrato et al., 2006)

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by Jra^Jbz.UAS, Scer\GAL4^pnr-MD237

(Cerrato et al., 2006)

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, enhanceable by kay^UAS.cEa, Scer\GAL4^pnr-MD237

(Cerrato et al., 2006)

NOT Enhanced by

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-enhanceable by Scer\GAL4^how-24B/Jra^UAS.cEa

(Cerrato et al., 2006)

NOT suppressed by

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by kay¹/kay[+]

(Cerrato et al., 2006)

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype, non-suppressible by Scer\GAL4^how-24B/Jra^UAS.cEa

(Cerrato et al., 2006)

Enhancer of

Statement

Reference

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 is an enhancer of adult thorax phenotype of Jra^Jbz.UAS, Scer\GAL4^pnr-MD237

(Cerrato et al., 2006)

Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 is an enhancer of adult thorax phenotype of Scer\GAL4^pnr-MD237, kay^UAS.cEa

(Cerrato et al., 2006)

Other

Statement

Reference

Jra^Asp.B.UAS, Mnn1^UAS.cCa, Scer\GAL4^pnr-MD237 has adult thorax phenotype

(Cerrato et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^pnr-MD237 in a Jra¹/+ background results in an enhanced thoracic cleft compared to Mnn1^Scer\UAS.cCa expression in a wild-type background.

The cleft thorax phenotype of flies that express either Mnn1^Scer\UAS.cCa or Jra^{bZIP.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 is enhanced when Mnn1^Scer\UAS.cCa and Jra^{bZIP.Scer\UAS} are expressed together by Scer\GAL4^pnr-MD237. Further, flies that coexpress Mnn1^Scer\UAS.cCa and Jra^{bZIP.Scer\UAS} under the control of Scer\GAL4^how-24B are lethal at pupal stage P7, which is earlier than the pharate adult lethality of Scer\GAL4^how-24B>Mnn1^Scer\UAS.cCa flies.

Coexpression of Mnn1^Scer\UAS.cCa and Jra^{Asp.B.Scer\UAS} under the control of Scer\GAL4^pnr-MD237 results in 1-5% of flies escaping the Jra^{Asp.B.Scer\UAS} lethality to eclose. Viable flies have a severe cleft thorax. Likewise, the prepupal lethality of Scer\GAL4^ey.PH>Jra^{Asp.B.Scer\UAS} flies is partially suppressed when Mnn1^Scer\UAS.cCa is coexpressed.

A kay¹/+ background has no effect on the Scer\GAL4^pnr-MD237>Mnn1^Scer\UAS.cCa phenotype.

Coexpression of kay^Scer\UAS.cEa and Mnn1^Scer\UAS.cCa under the control of Scer\GAL4^pnr-MD237 results in an enhancement of the cleft thorax phenotypes seen when either is expressed alone.

Coexpression of Mnn1^Scer\UAS.cCa with kay^{bZip.Scer\UAS}, under the control of Scer\GAL4^ey.PH, results in complete suppression of the Scer\GAL4^ey.PH>kay^{bZip.Scer\UAS} rough eye phenotype.

Coexpression of Mnn1^Scer\UAS.cCa and Jra^Scer\UAS.cEa under the control of either Scer\GAL4^pnr-MD237, Scer\GAL4^ey.PH or Scer\GAL4^how-24B results in the same phenotypes as expression of Mnn1^Scer\UAS.cCa alone.

(Cerrato et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mnn1^Scer\UAS.cCa

Mnn1^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct of a of Cerrato

Secondary FlyBase IDs

References (2)

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