Allele Dmel\mir-9a^J22

General Information
Symbol	Dmel\mir-9a^J22	Species	D. melanogaster
Name		FlyBase ID	FBal0198145
Feature type	allele	Associated gene	Dmel\mir-9a

Allele class
Mutagen	recombination, SCEI endonuclease, FLPase, ends-out gene targeting
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Li et al., 2006) FLPase (Li et al., 2006) recombination (Li et al., 2006) SCEI endonuclease (Li et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	mir-9a^{w.Scer\SceI.RS} (Li et al., 2006)
Nature of the lesion	Statement Reference An "ends-out" targeted recombination event at the mir-9a locus, using P{FRT(w⁺.mir-9a^{w.Scer\SceI.RS})} as the donor template, resulting in the replacement of the 78bp mir-9a precursor DNA sequence with w⁺ sequences. (Li et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Li et al., 2006) increased cell death \| third instar larval stage (with mir-9a^E39) (Bejarano et al., 2010) viable (Li et al., 2006) visible (Li et al., 2006, Biryukova et al., 2009, Bejarano et al., 2010) visible (with mir-9a^E39) (Bejarano et al., 2010) visible \| adult stage \| recessive (Loya et al., 2009)
Phenotype Manifest In
	abdominal dorsal multidendritic neuron ddaE \| ectopic (Li et al., 2006) abdominal dorsal multidendritic neuron ddaF \| ectopic (Li et al., 2006) anterior postalar bristle (with mir-9a^E39) (Bejarano et al., 2010) anterior wing margin (Bejarano et al., 2010) anterior wing margin (with mir-9a^E39) (Bejarano et al., 2010) dorsocentral bristle (with mir-9a^E39) (Bejarano et al., 2010) eo neuron \| ectopic (Li et al., 2006) microchaeta (Bejarano et al., 2010) microchaeta (with mir-9a^E39) (Bejarano et al., 2010) posterior wing margin (Li et al., 2006, Bejarano et al., 2010) posterior wing margin (with mir-9a^E39) (Bejarano et al., 2010) scutellar bristle \| ectopic (Li et al., 2006) sensory mother cell \| ectopic (Li et al., 2006) tormogen cell \| ectopic (Li et al., 2006) trichogen cell \| ectopic (Li et al., 2006) wing margin (Biryukova et al., 2009) wing margin \| posterior (Loya et al., 2009) wing margin bristle \| ectopic & anterior wing margin (Li et al., 2006) wing pouch (with mir-9a^E39) (Bejarano et al., 2010)
Detailed Description
	Statement Reference Homozygotes and mir-9a[J22]/mir-9a[E39] animals lack approximately 50% of the posterior wing margin in all individuals and approximately 10% of animals also show mild loss of anterior wing margin. Abundant cell death is seen in the wing pouch region of the wing disc in mir-9a[J22]/mir-9a[E39] third instar larvae, in contrast to wild-type controls. Cell death is not seen in the pronotum region of the disc in the mutant larvae. Homozygous and mir-9a[J22]/mir-9a[E39] adults show a mild increase in notum microchaete density compared to controls. mir-9a[J22]/mir-9a[E39] adults often have ectopic dorsocentral and anterior postalar bristles. (Bejarano et al., 2010) mir-9a[J22] homozygous flies lack anterior and posterior wing margins. Heterozygotes show normal wing development. (Biryukova et al., 2009) Homozygous mir-9a[J22] mutants exhibit posterior wing margin defects. No defects are seen in heterozygous mir-9a[J22] flies. (Loya et al., 2009) 29% of mir-9a^J22 third instar larvae have an extra ddaE or ddaF neuron in one or two but not other hemisegments. Mutant adults lack the posterior wing margin (100% penetrance), while the veins are normal in appearance. The anterior wing margin contains ectopic sensory bristles in about 40% of mutant flies. 14.3% of mutant flies have 3-4 scutellar bristles per heminotum (instead of the wild-type number of 2 per heminotum). These extra bristles have normal shaft and socket cells and are accompanied by a sensory neuron. Mutant third instar wing discs have a high frequency of ectopic sensory organ precursors (SOPs) in the notum region. (Li et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible \| partially by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible \| partially by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^Scer\UAS.cHa (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has visible phenotype, suppressible by Scer\GAL4^ap-md544/th^Scer\UAS.cHa (Bejarano et al., 2010) mir-9a^J22 has increased cell death phenotype, suppressible by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22 has visible phenotype, suppressible \| partially by sens^E58/sens[+] (Li et al., 2006) mir-9a^J22 has visible phenotype, suppressible by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22 has visible phenotype, suppressible by Bx^hdp-n60 (Biryukova et al., 2009)
NOT suppressed by
Statement Reference mir-9a^J22 has increased cell death phenotype, non-suppressible by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22 has visible \| recessive \| adult stage phenotype, non-suppressible by Scer\GAL4^vg.PM/Avic\GFP^{EGFP.Scer\UAS-mir-9aSP} (Loya et al., 2009)
Enhancer of
Statement Reference mir-9a^J22/mir-9a[+] is an enhancer of visible \| adult stage phenotype of Avic\GFP^{EGFP.Scer\UAS-mir-9aSP}, Scer\GAL4^αTub84B.PL (Loya et al., 2009) mir-9a^J22/mir-9a[+] is an enhancer of visible phenotype of Bx^D124 (Biryukova et al., 2009) mir-9a^J22/mir-9a[+] is an enhancer of visible phenotype of Bx^D128 (Biryukova et al., 2009)
Phenotype Manifest In
Suppressed by
Statement Reference mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible \| partially by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has anterior postalar bristle phenotype, suppressible \| partially by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has dorsocentral bristle phenotype, suppressible \| partially by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has wing disc phenotype, suppressible by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible \| partially by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has wing margin phenotype, suppressible by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/BacA\p35^Scer\UAS.cHa (Bejarano et al., 2010) mir-9a^J22/mir-9a^E39 has wing phenotype, suppressible by Scer\GAL4^ap-md544/th^Scer\UAS.cHa (Bejarano et al., 2010) mir-9a^J22 has wing margin phenotype, suppressible \| partially by sens^E58/sens[+] (Li et al., 2006) mir-9a^J22 has wing margin phenotype, suppressible by Bx[+]/Bx^hdpR26 (Bejarano et al., 2010) mir-9a^J22 has wing margin phenotype, suppressible by Bx^hdp-n60 (Biryukova et al., 2009)
NOT suppressed by
Statement Reference mir-9a^J22/mir-9a^E39 has wing pouch phenotype, non-suppressible by sens^E2/sens[+] (Bejarano et al., 2010) mir-9a^J22 has wing margin \| posterior phenotype, non-suppressible by Scer\GAL4^vg.PM/Avic\GFP^{EGFP.Scer\UAS-mir-9aSP} (Loya et al., 2009)
Enhancer of
Statement Reference mir-9a^J22/mir-9a[+] is an enhancer of wing margin \| posterior phenotype of Avic\GFP^{EGFP.Scer\UAS-mir-9aSP}, Scer\GAL4^αTub84B.PL (Loya et al., 2009) mir-9a^J22/mir-9a[+] is an enhancer of wing margin phenotype of Bx^D124 (Biryukova et al., 2009) mir-9a^J22/mir-9a[+] is an enhancer of wing margin phenotype of Bx^D128 (Biryukova et al., 2009)
Additional Comments
Genetic Interactions
Statement Reference Expression of th[Scer\UAS.cHa] under the control of Scer\GAL4[ap-md544] completely rescues the wing notching phenotype of mir-9a[J22]/mir-9a[E39] animals. sens[E2]/+ results in an approximately 35% reduction in the loss of wing margin caused by mir-9a[J22]/mir-9a[E39]. sens[E2]/+ does not rescue the increased cell death seen in the wing discs of mir-9a[J22]/mir-9a[E39] animals. Bx[hdpR26]/+ fully rescues the adult wing margin defects of mir-9a[J22] homozygotes and mir-9a[J22]/mir-9a[E39] animals. Bx[hdpR26]/+ strongly suppresses the ectopic apoptosis seen in the wing discs of mir-9a[J22]/mir-9a[E39] animals. The presence of Bx[hdpR26]/+ partially suppresses the formation of ectopic anterior postalar bristles seen in mir-9a[J22]/mir-9a[E39] adults, but has only mild effects on the formation of ectopic dorsocentral bristles seen in these flies. sens[E2]/+ partially suppresses the formation of ectopic dorsocentral and anterior postalar bristles seen in mir-9a[J22]/mir-9a[E39] adults. (Bejarano et al., 2010) The loss of wing margin seen in mir-9a[J22] flies is completely suppressed by Bx[hdp-n60]. The wing margin phenotype seen in Bx[D124]/Y and Bx[D128]/Y flies is enhanced by mir-9a[J22]/+. (Biryukova et al., 2009) One copy of sens^E58 substantially rescues the wing margin defects of mir-9a^J22 homozygotes; about 30% of the double mutant flies have wings that are indistinguishable from wild type, while others lack only a small part of the posterior margin. The bristles on the anterior wing margin resemble wild type in the double mutant flies. (Li et al., 2006)
Xenogenetic Interactions
Statement Reference Expression of BacA\p35[Scer\UAS.cHa] under the control of Scer\GAL4[ap-md544] completely rescues the wing notching phenotype of mir-9a[J22]/mir-9a[E39] animals. (Bejarano et al., 2010) A mir-9a[J22]/+ background enhances the wing margin defects seen when Avic\GFP[EGFP.Scer\UAS-mir-9aSP] is expressed under the control of Scer\GAL4[αTub84B.PL]. Expression of Avic\GFP[EGFP.Scer\UAS-mir-9aSP] along the dorsal-ventral boundary of the wing disc using Scer\GAL4[vg.PM] fails to rescue the wing margin phenotype seen in mir-9a[J22] homozygous mutants. (Loya et al., 2009)
Complementation & Rescue Data
Rescued by	mir-9a^J22/mir-9a^E39 is rescued by Scer\GAL4^ap-md544/mir-9a^Scer\UAS.cLa (Bejarano et al., 2010)
Partially rescued by	mir-9a^J22 is partially rescued by mir-9a^Scer\UAS.cLa/Scer\GAL4^vg.PU (Li et al., 2006)
Comments	Expression of mir-9a[Scer\UAS.cLa] under the control of Scer\GAL4[ap-md544] completely rescues the continuity of the wing margin in mir-9a[J22]/mir-9a[E39] animals. In the developing wing disc, the increased apoptosis seen in the wing pouch in mir-9a[J22]/mir-9a[E39] animals is rescued in the dorsal compartment by expression of mir-9a[Scer\UAS.cLa] under the control of Scer\GAL4[ap-md544]. (Bejarano et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	J22 (Li et al., 2006) mir-9a^J22 (Biryukova et al., 2009, Bejarano et al., 2010) miR-9a^J22 (Loya et al., 2009)
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Bejarano et al., 2010, Dev. Biol. 338(1): 63--73 miR-9a prevents apoptosis during wing development by repressing Drosophila LIM-only. [FBrf0209786] Biryukova et al., 2009, Dev. Biol. 327(2): 487--496 Drosophila mir-9a regulates wing development via fine-tuning expression of the LIM only factor, dLMO. [FBrf0207536] Loya et al., 2009, Nat. Methods 6(12): 897--903 Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms. [FBrf0209364] Li et al., 2006, Genes Dev. 20(20): 2793--2805 MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila. [FBrf0193418]

Allele Dmel\mir-9aJ22

Allele Dmel\mir-9a^J22