Allele Dmel\mir-9a^Scer\UAS.cLa

General Information
Symbol	Dmel\mir-9a^Scer\UAS.cLa	Species	D. melanogaster
Name		FlyBase ID	FBal0198150
Feature type	allele	Associated gene	Dmel\mir-9a

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Li et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of the wild-type 78bp mir-9a precursor. (Li et al., 2006)
Carried in construct	P{UAS-mir-9a.L} (Li et al., 2006, Xu et al., 2008, Bejarano et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective, with Scer\GAL4²²¹ (Xu et al., 2008) visible, with Scer\GAL4^ap-md544 (Li et al., 2006)
Phenotype Manifest In
	abdominal 1 dorsal multidendritic neuron ddaE, with Scer\GAL4²²¹ (Xu et al., 2008) abdominal 1 dorsal multidendritic neuron ddaF, with Scer\GAL4²²¹ (Xu et al., 2008) dendrite, with Scer\GAL4²²¹ (Xu et al., 2008) dendritic arborizing neuron, with Scer\GAL4²²¹ (Xu et al., 2008) scutellar bristle, with Scer\GAL4^ap-md544 (Li et al., 2006) sensory mother cell, with Scer\GAL4^ap-md544 (Li et al., 2006)
Detailed Description
	Statement Reference Ectopic expression of mir-9a[Scer\UAS.cLa] in ddaE, ddaF and DA neurons in the dorsal cluster under the control of Scer\GAL4[221] significantly increases the number of dendritic ends of both ddaE and ddaF neurons. (Xu et al., 2008) Expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 results in a loss of scutellar bristles in adult flies. Expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^ap-md544 results in the loss of sensory organ precursors in the notum region of the wing disc. (Li et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference mir-9a^Scer\UAS.cLa/Scer\GAL4^vg.PU is a suppressor \| partially of visible phenotype of Scer\GAL4^vg.PU, sens^Scer\UAS.cNa (Li et al., 2006)
Phenotype Manifest In
Suppressor of
Statement Reference mir-9a^Scer\UAS.cLa/Scer\GAL4^vg.PU is a suppressor \| partially of chaeta \| ectopic phenotype of Scer\GAL4^vg.PU, sens^Scer\UAS.cNa (Li et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of mir-9a^Scer\UAS.cLa results in the suppression of the ectopic bristle phenotype caused by expression of sens^Scer\UAS.cNa under the control of Scer\GAL4^vg.PU. (Li et al., 2006)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Scer\GAL4^ap-md544/mir-9a^Scer\UAS.cLa rescues mir-9a^J22/mir-9a^E39 (Bejarano et al., 2010)
Partially rescues	mir-9a^Scer\UAS.cLa/Scer\GAL4^vg.PU partially rescues mir-9a^J22 (Li et al., 2006)
Comments	Expression of mir-9a[Scer\UAS.cLa] under the control of Scer\GAL4[ap-md544] completely rescues the continuity of the wing margin in mir-9a[J22]/mir-9a[E39] animals. In the developing wing disc, the increased apoptosis seen in the wing pouch in mir-9a[J22]/mir-9a[E39] animals is rescued in the dorsal compartment by expression of mir-9a[Scer\UAS.cLa] under the control of Scer\GAL4[ap-md544]. (Bejarano et al., 2010) The posterior wing margin defects seen in mir-9a^J22 flies are are completely rescued by expression of mir-9a^Scer\UAS.cLa under the control of Scer\GAL4^vg.PU, and the anterior margin bristle defects are also largely rescued. (Li et al., 2006)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	mir-9a^Scer\UAS.cLa
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Bejarano et al., 2010, Dev. Biol. 338(1): 63--73 miR-9a prevents apoptosis during wing development by repressing Drosophila LIM-only. [FBrf0209786] Xu et al., 2008, J. Neurosci. 28(46): 11883--11889 The steady-state level of the nervous-system-specific microRNA-124a is regulated by dFMR1 in Drosophila. [FBrf0206327] Li et al., 2006, Genes Dev. 20(20): 2793--2805 MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila. [FBrf0193418]

Allele Dmel\mir-9aScer\UAS.cLa

Allele Dmel\mir-9a^Scer\UAS.cLa