Ooplasmic streaming occurs in the oocytes of αTub67Ckav-rX21/Df(3L)55 females, although the average streaming velocity is 317+/-8 nm/second compared to 348+/-5 nm/second in control females. The average velocity of lipid droplets in the mutant early stage 12 egg chambers is significantly reduced compared to controls, while the average dwell time and average run length are not significantly different from wild type.
Early stage 12 egg primordia derived from αTub67Ckav-rX21/αTub67Ckav-18c females show no sign of the fast, linear motion of the non-streaming lipid droplet subpopulation that is seen in control egg primordia.
Although eggs laid by αTub67Ckav-rX21/+ females are fertilized, embryogenesis does not begin. The centrosome introduced by the sperm is duplicated and daughter centrosomes form, but these daughter centrosomes fail to move apart along the nuclear envelope and no spindle can be seen between them. Instead they remain closely associated with a tassel of microtubules with over-condensed chromosomes inside - indicative of a collapsed spindle