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General Information
Symbol
Dmel\αTub67Ckav-rX21
Species
D. melanogaster
Name
FlyBase ID
FBal0198519
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
kavarnull, kavarrX21
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Deletion of four base pairs (CTGC) leads to a frameshift from the 19th codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Deletion of 4bp near the 5' end of the αTub67C ORF (C56TGC) leads to frameshift from the 19th codon. The predicted product is only 34 amino acids long.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos of αTub67Ckav-rX21 hemizygous mothers display an abnormal cortical microtubule network. A network of only short microtubules without directionality is observed.

Ooplasmic streaming occurs in the oocytes of αTub67Ckav-rX21/Df(3L)55 females, although the average streaming velocity is 317+/-8 nm/second compared to 348+/-5 nm/second in control females. The average velocity of lipid droplets in the mutant early stage 12 egg chambers is significantly reduced compared to controls, while the average dwell time and average run length are not significantly different from wild type.

Early stage 12 egg primordia derived from αTub67Ckav-rX21/αTub67Ckav-18c females show no sign of the fast, linear motion of the non-streaming lipid droplet subpopulation that is seen in control egg primordia.

Microtubule formation following initiation is slower in extracts from αTub67Ckav-rX21/Df(3L)55 embryos than in equivalent extracts from wild-type embryos.

Although eggs laid by αTub67Ckav-rX21/+ females are fertilized, embryogenesis does not begin. The centrosome introduced by the sperm is duplicated and daughter centrosomes form, but these daughter centrosomes fail to move apart along the nuclear envelope and no spindle can be seen between them. Instead they remain closely associated with a tassel of microtubules with over-condensed chromosomes inside - indicative of a collapsed spindle

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Induced in a screen for reversion of dominant female sterile phenotype of αTub67Ckav-21g as described in FBrf0051088.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (5)