FlyBase Allele Report: Dmel\α-Cat[GD8808]

General Information

Symbol

Dmel\α-Cat^GD8808

Species

D. melanogaster

Name

FlyBase ID

FBal0198530

Feature type

allele

Associated gene

Dmel\α-Cat

Associated Insertion(s)

Carried in Construct

P{GD8808}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8808}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8808

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^tsh-md621

(Petzoldt et al., 2012)

lethal, with Scer\GAL4^Tub.PU

(Syrzycka et al., 2019)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adherens junction | somatic clone

(Sarpal et al., 2012)

egg chamber, with Scer\GAL4^NP1624

(Sarpal et al., 2012)

male genitalia, with Scer\GAL4^Abd-B-LDN

(Petzoldt et al., 2012)

male genitalia, with Scer\GAL4^NP1548

(Petzoldt et al., 2012)

ovary, with Scer\GAL4^NP1624

(Sarpal et al., 2012)

Detailed Description

Statement

Reference

Males expressing α-Cat^GD8808 under the control of Scer\GAL4^{Myo31DF-NP1548} sometimes show partial rotation of the genitalia.

Males expressing α-Cat^GD8808 under the control of Scer\GAL4^Abd-B-LDN show partial rotation of the genitalia.

(Petzoldt et al., 2012)

Expression of α-Cat^GD8808 in follicle cells under the control of Scer\GAL4^tj-NP1624 produces oogenesis defects. Ovarioles frequently form a single giant fused egg chamber that contains multiple germline cysts.

Follicle cell clones expressing α-Cat^GD8808 causes loss of adherens junctions and formation of α-Spec aggregates.

(Sarpal et al., 2012)

Adults expressing α-Cat^GD8808 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8808

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

α-Cat^GD8808, Scer\GAL4^NP1548, shg^KK103334 has lethal phenotype

(Petzoldt et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

α-Cat^GD8808 has adherens junction | somatic clone phenotype, non-enhanceable by SCAR^GD11380

(Sarpal et al., 2012)

Other

Statement

Reference

α-Cat^GD8808, Scer\GAL4^Abd-B-LDN, shg^KK103334 has male genitalia phenotype

(Petzoldt et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Males co-expressing shg^KK103334 and α-Cat^GD8808 under the control of Scer\GAL4^Abd-B-LDN have defects in genital rotation, either showing partial (77%) or no (23%) rotation.

(Petzoldt et al., 2012)

Co-expression of SCAR^GD11380 does not enhance the adherens junction phenotype seen in follicle cell clones expressing α-Cat^GD8808.

Co-expression of Arpc1^KK108277 does not enhance the adherens junction phenotype seen in follicle cell clones expressing α-Cat^GD8808.

(Sarpal et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type