FlyBase Allele Report: Dmel\αCOP[GD12332]

General Information

Symbol

Dmel\αCOP^GD12332

Species

D. melanogaster

Name

FlyBase ID

FBal0198546

Feature type

allele

Associated gene

Dmel\αCOP

Associated Insertion(s)

Carried in Construct

P{GD12332}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12332}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12332

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Phenotype Manifest In

astral microtubule | male, with Scer\GAL4^VP16.bam

(Kitazawa et al., 2012)

onion stage spermatid, with Scer\GAL4^VP16.bam

(Kitazawa et al., 2012)

spindle microtubule | male, with Scer\GAL4^VP16.bam

(Kitazawa et al., 2012)

Detailed Description

Statement

Reference

Males expressing αCop^GD12332 under the control of Scer\GAL4^{bam.T:Hsim\VP16} show cytokinesis defects in onion-stage spermatids: spermatids containing one Nebenkern and more than one nucleus are seen.

Markedly fewer astral and spindle microtubules than normal are constructed after breakdown of the nuclear membrane in spermatocytes expressing αCop^GD12332 under the control of Scer\GAL4^{bam.T:Hsim\VP16}, although the bipolar spindle is initially formed. Following anaphase, the spindle microtubules elongate in an aberrant direction. The interdigitated microtubules from opposite poles fail to overlap. These microtubules become bent and eventually degrade. The primary spermatocytes do not elongate along the spindle axis after anaphase and no cleavage furrow ingression occurs.

(Kitazawa et al., 2012)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early larval lethality or late larval lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development