Expression of βTub60D^GD10956 under the control of Scer\GAL4^elav-C155 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a severe, almost complete loss of microtubules at the larval neuromuscular junction, and a significant increase in synaptic retraction frequency.

Adults expressing βTub60D^GD10956 under the simultaneous control of both Scer\GAL4^repo.PL and Scer\GAL4^repo show a highly penetrant spastic locomotion defect.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in extreme defects in the shape of neuroblasts in the larval brain.

Adults expressing βTub60D^GD10956 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of βTub60D^GD10956 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD10956} insertion line used.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 40-50% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% or 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.