FlyBase Allele Report: Dmel\Abd-B[GD4680]

General Information

Symbol

Dmel\Abd-B^GD4680

Species

D. melanogaster

Name

FlyBase ID

FBal0198635

Feature type

allele

Associated gene

Dmel\Abd-B

Associated Insertion(s)

Carried in Construct

P{GD4680}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4680}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4680

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mating behavior, with Ecol\lexA^p65.R57C10.P, Scer\FLP1^lexAop.cSa, Scer\GAL4^Abd-B-LDN, Scer\GAL80^αTub84B.PG

(Bussell et al., 2014)

abnormal mating behavior, with Scer\GAL4^elav.PLu

(Bussell et al., 2014)

abnormal mating behavior, with Scer\GAL4^nSyb.PS

(Bussell et al., 2014)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

increased cell number | adult stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

increased cell number | third instar larval stage, with Scer\GAL4^elav-C155

(Estacio-Gómez et al., 2013)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Abd-B^GD4680 under the control of Scer\GAL4^elav.PLu or Scer\GAL4^nSyb.PS (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in a significant decrease in virgin female receptivity, as compared to controls (but this phenotype is not seen if a Gal80[ts] transgene is included to restrict expression to the adult stage); no significant difference in the postmating response is seen in these flies, and there is no difference in female attractiveness to males as measured by male courtship index, as compared to controls.

Expression of Abd-B^GD4680 under the control of Scer\GAL4^Abd-B-LDN (along with Scer\FLP1^lexAop.cSa, Scer\GAL80^αTub84B.PG and Ecol\lexA^p65.R57C10.P to restrict expression to Abd-B-expressing neurons) in virgin females results in significantly reduced receptivity to mating, as compared to controls. Expression of Abd-B^GD4680 under the control of Scer\GAL4^Abd-B-LDN alone results in females with malformed genitalia that are unable to copulate.

(Bussell et al., 2014)

Adults expressing Abd-B^GD4680 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4680

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Abd-B^GD4680, Scer\GAL4^insc-Mz1407, dsx^UAS.female has decreased cell number | larval stage phenotype

(Ghosh et al., 2019)

Abd-B^GD4680, Scer\GAL4^insc-Mz1407, dsx^UAS.female has abnormal cell death | female limited | larval stage phenotype

(Ghosh et al., 2019)

Abd-B^GD4680, Scer\GAL4^elav-C155, Ubx^RNAi.UAS has abnormal neuroanatomy | adult stage phenotype

(Estacio-Gómez et al., 2013)

Abd-B^GD4680, Scer\GAL4^elav-C155, Ubx^RNAi.UAS has decreased cell number | adult stage phenotype

(Estacio-Gómez et al., 2013)

Phenotype Manifest In

Other

Statement

Reference

Abd-B^GD4680, Scer\GAL4^insc-Mz1407, dsx^UAS.female has thoracic neuroblast | larval stage phenotype

(Ghosh et al., 2019)

Abd-B^GD4680, Scer\GAL4^insc-Mz1407, dsx^UAS.female has larval terminal neuroblast | female limited phenotype

(Ghosh et al., 2019)

Abd-B^GD4680, Scer\GAL4^elav-C155, Ubx^RNAi.UAS has adult ventral nerve cord phenotype

(Estacio-Gómez et al., 2013)

Abd-B^GD4680, Scer\GAL4^elav-C155, Ubx^RNAi.UAS has adult abdominal ganglion Leucokinin neuron | decreased number phenotype

(Estacio-Gómez et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Abd-B^GD4680 and dsx^UAS.female, in combination with a Dicer-2 transgene to enhance RNAi efficiency, under the control of Scer\GAL4^insc-Mz1407 from early first instar stage, in combination with a Gal80[ts] transgene, prevents female-specific loss of larval terminal neuroblasts, compared to controls; there is also a significant decrease in the number of thoracic neuroblasts in the female, compared to controls.

(Ghosh et al., 2019)

Expression of Abd-B^GD4680 RNAi under the control of Scer\GAL4^lama-A8 in the Pcl^3-78.38 homozygous mutant somatic clones induced specifically in brain optic lobes prevents the formation of characteristic for Pcl^3-78.38 clones.

(Apitz and Salecker, 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12024

w¹¹¹⁸; P{GD4680}v12024/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4680

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Abd-B^GD4680

Abd-B^IR

(Apitz and Salecker, 2016)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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