Allele Dmel\Actn^GD1354

General Information
Symbol	Dmel\Actn^GD1354	Species	D. melanogaster
Name		FlyBase ID	FBal0198670
Feature type	allele	Associated gene	Dmel\Actn

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..273
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD1354} (Dickson et al., 2007.7.18, Dietzl et al., 2007, Schnorrer et al., 2010, Mummery-Widmer et al., 2009)
Associated Sequence Features	dsRNA-GD1354
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act5C.PU (Dietzl et al., 2007) lethal, with Scer\GAL4^Mef2.PR (Dietzl et al., 2007) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	eye, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) Z disc, with Scer\GAL4^Mef2.PR (Schnorrer et al., 2010)
Detailed Description
	Statement Reference Larvae expressing Actn[GD1354] under the control of Scer\GAL4[Mef2.PR] have muscles with "fading Z" sarcomeres (the Z-lines are reduced). (Schnorrer et al., 2010) Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 0% or 90-100% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in the absence of 0% or 10-20% of the Scer\GAL4[pnr-MD237]-expressing area of the notum, depending on the insertion line used. (Mummery-Widmer et al., 2009) Expression of argos^GD1666 under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF leads to a mild rough eye phenotype. (Dietzl et al., 2007)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1354
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Actn^GD1354, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH has eye phenotype, enhanceable by Dcr-2^Scer\UAS.cDa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference Coexpression of Dcr-2^Scer\UAS.cDa with Actn^GD1354, under the control of Scer\GAL4^ey.PH and Scer\GAL4^GMR.PF, leads to an enhancement of the Actn^GD1354 rough eye phenotype. (Dietzl et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
VDRC	v7760 w¹¹¹⁸; P{GD1354}v7760 v7761 w¹¹¹⁸; P{GD1354}v7761 v7762 w¹¹¹⁸; P{GD1354}v7762
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1354
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Actn^GD1354
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Schnorrer et al., 2010, Nature 464(7286): 287--291 Systematic genetic analysis of muscle morphogenesis and function in Drosophila. [FBrf0210226] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\ActnGD1354

Allele Dmel\Actn^GD1354