Expression of Adar^GD1355 under the control of Scer\GAL4^eve.RRa leads to significantly increased firing activity of larval aCC neurons.

Knockdown of Adar through expression of two copies of Adar^GD1355 in fru neurons under the control of Scer\GAL4^fru.P1.B does not significantly alter male locomotor activity, latency to court, or total time spent courting. Male-male courting is not observed in these males. This, as well as the robust courtship of females, indicates that the development and wiring of fru neurons are unlikely to be affected by Adar knockdown.

Males expressing two copies of Adar^GD1355 in fru neurons under the control of Scer\GAL4^fru.P1.B exhibit polycyclic waveforms and/or additional peaks that are not observed in controls in approximately 44% of song trains. This is accompanied by an increase in the average number of pulses per song train but no significant alteration in either pulse frequency or inter-pulse interval relative to controls.

Adults expressing Adar^GD1355 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies.

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 40-50% or 70-80% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% or 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.