Allele Dmel\Akt1^GD1361

General Information
Symbol	Dmel\Akt1^GD1361	Species	D. melanogaster
Name		FlyBase ID	FBal0198698
Feature type	allele	Associated gene	Dmel\Akt1

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..277
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD1361} (Dickson et al., 2007.7.18, Dietzl et al., 2007, Parrish et al., 2009, Shelly et al., 2009, Wang et al., 2008, Resnik-Docampo and de Celis, 2011, Mummery-Widmer et al., 2009, Nechipurenko and Broihier, 2012, Sinenko et al., 2011, Song et al., 2012, Sinenko et al., 2010, Sinenko et al., 2011, Biteau et al., 2010)
Associated Sequence Features	dsRNA-GD1361
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell number, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) decreased cell size, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) lethal, with Scer\GAL4^Act5C.PU (Dietzl et al., 2007) lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH (Dietzl et al., 2007) lethal, with Scer\GAL4^pnr-MD237 (Dietzl et al., 2007) lethal \| pupal stage, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) long lived \| RU486 conditional, with Scer\GAL4^bun-GSG5961 (Biteau et al., 2010) neuroanatomy defective \| larval stage, with Scer\GAL4^ppk.PG (Parrish et al., 2009) short lived \| heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Biteau et al., 2010) size defective, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Phenotype Manifest In
	adult gut \| heat sensitive, with Scer\GAL4^esg-NP5130, Scer\GAL80^ts.αTub84B (Biteau et al., 2010) adult gut \| RU486 conditional, with Scer\GAL4^bun-GSG5961 (Biteau et al., 2010) dendrite \| larval stage, with Scer\GAL4^ppk.PG (Parrish et al., 2009) wing, with Scer\GAL4^Bx-MS1096 (Dietzl et al., 2007) wing, with Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Detailed Description
	Statement Reference The dendrites of ddaC neurons of larvae expressing Akt1[GD1361] under the control of Scer\GAL4[ppk.PG] show reduced regeneration (after dendrite severing at 48 hours after egg laying) compared to wild-type controls. (Song et al., 2012) Expression of Akt1[GD1361] using Scer\GAL4[nub-AC-62] results in abnormally small wings in which cell size and cell number are decreased. (Resnik-Docampo and de Celis, 2011) Compared with controls, the age-related dysplasia of the intestinal epithelium is strongly reduced in flies expressing Akt1[GD1361] under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B]. Expression of Akt1[GD1361] under the control of Scer\GAL4[esg-NP5130] and Scer\GAL80[ts.αTub84B] results in significant lifespan shortening compared with controls. Compared with controls, female flies expressing Akt1[GD1361] under the control of Scer\GAL4[bun-GSG5961] show a moderate, but significant, reduction in intestinal cell proliferation at old age. These flies are significantly longer lived when exposed to RU486 than isogenic siblings exposed to mock treatment. (Biteau et al., 2010) Knocking down Akt1 expression via Scer\GAL4[ppk.PG]-driven expression of Akt1[GD1361] in class IV neurons causes a significant reduction in dendrite growth and dendrite coverage of larval hemisegments. (Parrish et al., 2009) Expression of Akt1^GD1361 under the control of Scer\GAL4^Bx-MS1096 leads to crinkled wings. (Dietzl et al., 2007)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1361
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference Akt1^GD1361, Scer\GAL4^nub-AC-62 has decreased cell number phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) Akt1^GD1361, Scer\GAL4^nub-AC-62 has decreased cell size phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011) Akt1^GD1361, Scer\GAL4^nub-AC-62 has size defective phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Enhancer of
Statement Reference Akt1^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell number phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011) Akt1^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of decreased cell size phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011) Akt1^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of size defective phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011)
Suppressor of
Statement Reference Akt1^GD1361, Scer\GAL4^Hml.Δ is a suppressor \| partially of increased cell number \| third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa, Scer\GAL4^Hml.Δ (Sinenko et al., 2010)
Phenotype Manifest In
Enhanced by
Statement Reference Akt1^GD1361, Scer\GAL4^nub-AC-62 has wing phenotype, enhanceable by hppy^Scer\UAS.cMa, Scer\GAL4^nub-AC-62 (Resnik-Docampo and de Celis, 2011)
Enhancer of
Statement Reference Akt1^GD1361, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, Crtc^Scer\UAS.cWa (Wang et al., 2008) Akt1^GD1361, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Scer\GAL4^nub-AC-62, hppy^Scer\UAS.cMa (Resnik-Docampo and de Celis, 2011)
Suppressor of
Statement Reference Akt1^GD1361, Scer\GAL4^Dot.PK is a suppressor of lamellocyte \| larval stage phenotype of ND75^GD6309, Scer\GAL4^Dot.PK (Sinenko et al., 2011) Akt1^GD1361, Scer\GAL4^Hml.Δ is a suppressor \| partially of embryonic/larval hemocyte \| third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa, Scer\GAL4^Hml.Δ (Sinenko et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of hppy[Scer\UAS.cMa] and Akt1[GD1361] using Scer\GAL4[nub-AC-62] results in a strong synergistic phenotype of reduced wing size and reduced cell size and number. (Resnik-Docampo and de Celis, 2011) Expression of Akt1[GD1361] suppresses the increase in circulating lamellocytes seen when ND75[GD6309] is expressed under the control of Scer\GAL4[Dot.PK]. (Sinenko et al., 2011) The Scer\GAL4[ppk.PG]-driven Akt1[GD1361]-phenotype is epistatic to the dendrite overgrowth seen in Df(3L)ban[Δ1] mutants. In addition, reducing Akt1 expression by Scer\GAL4[ppk.PG]-driven Akt1[GD1361] blocks the exuberant dendrite invasion activity of Df(3L)ban[Δ1] mutants. (Parrish et al., 2009) Depletion of Akt1 through expression of Akt1[GD1361] in TORC[Scer\UAS.cWa]-expressing developing eyes (both lines under the control of Scer\GAL4[GMR.PF]) enhances the TORC-mediated rough eye phenotype. (Wang et al., 2008)
Xenogenetic Interactions
Statement Reference Expression of Akt1[GD1361] strongly suppresses the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1[Scer\UAS.cSa] is expressed under the control of Scer\GAL4[Hml.Δ]. (Sinenko et al., 2010)
Complementation & Rescue Data
Comments
Stocks ( 1 )
VDRC	v2902 w¹¹¹⁸; P{GD1361}v2902
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 1361
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Akt1^GD1361
Name Synonym
Secondary FlyBase IDs

References ( 13 )
Research paper	Nechipurenko and Broihier, 2012, J. Cell Biol. 196(3): 345--362 FoxO limits microtubule stability and is itself negatively regulated by microtubule disruption. [FBrf0217391] Song et al., 2012, Genes Dev. 26(14): 1612--1625 Regeneration of Drosophila sensory neuron axons and dendrites is regulated by the Akt pathway involving Pten and microRNA bantam. [FBrf0218927] Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911] Sinenko et al., 2011, EMBO Rep. 13(1): 83--89 Oxidative stress in the haematopoietic niche regulates the cellular immune response in Drosophila. [FBrf0217071] Biteau et al., 2010, PLoS Genet. 6(10): e1001159 Lifespan extension by preserving proliferative homeostasis in Drosophila. [FBrf0212112] Sinenko et al., 2010, Blood 116(22): 4612--4620 Genetic manipulation of AML1-ETO-induced expansion of hematopoietic precursors in a Drosophila model. [FBrf0212361] Parrish et al., 2009, Neuron 63(6): 788--802 The microRNA bantam functions in epithelial cells to regulate scaling growth of dendrite arbors in drosophila sensory neurons. [FBrf0208858] Shelly et al., 2009, Immunity 30(4): 588--598 Autophagy is an essential component of Drosophila immunity against vesicular stomatitis virus. [FBrf0207677] Wang et al., 2008, Cell Metab. 7(5): 434--444 The insulin-regulated CREB coactivator TORC promotes stress resistance in Drosophila. [FBrf0204686] Dietzl et al., 2007, Nature 448(7150): 151--156 A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. [FBrf0200691]
Supplementary material	Sinenko et al., 2011, EMBO Rep. 13(1): Supplementary information. [FBrf0219127] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\Akt1GD1361

Allele Dmel\Akt1^GD1361