FlyBase Allele Report: Dmel\Ref1[GD4683]

General Information

Symbol

Dmel\Ref1^GD4683

Species

D. melanogaster

Name

FlyBase ID

FBal0198713

Feature type

allele

Associated gene

Dmel\Ref1

Associated Insertion(s)

Carried in Construct

P{GD4683}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4683}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4683

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal planar polarity, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

female semi-sterile, with Scer\GAL4^nanos.PG

(Czech et al., 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Ref1^GD4683 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing Ref1^GD4683 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in duplication of bristle hairs on the notum in 0-10% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4683

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Ref1^GD4683, Scer\GAL4^da.G32 is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Scer\GAL4^da.G32, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

Ref1^GD4683, Scer\GAL4^nSyb.PS is a non-suppressor of abnormal locomotor behavior | third instar larval stage phenotype of Scer\GAL4^nSyb.PS, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

Ref1^GD4683, Scer\GAL4^GMR.PF is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Ref1^GD4683, Scer\GAL4^GMR.PF is a non-suppressor of eye | adult stage phenotype of Scer\GAL4^GMR.PF, Zzzz\GGGGCC^36.UAS

(Hautbergue et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Ref1^GD4683 does not suppress the rough eye, the decreased adult locomotor activity or the decreased larval locomotor activity induced by the expression of Zzzz\GGGGCC^36.Scer\UAS under the control of Scer\GAL4^GMR.PF, Scer\GAL4^da.G32, and Scer\GAL4^nSyb.PS, respectively.

(Hautbergue et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v12031

w¹¹¹⁸ P{GD4683}v12031

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4683

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Aly^GD4683

Ref1^GD4683

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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