Adulthood-only expression of Atg1GD7149 under the control of Scer\GAL4esg.PU leads to a severe increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).
The expression of Atg1GD7149, driven by Scer\GAL4NP0001 or driven by Scer\GAL4hs.PB and induced by 1h heat-shocka during the 2 days before the experiment, but not when driven by Scer\GAL4ppl.PU, induces a significantly higher mortality to Ecc15 enteric infection, as compared to controls; the Scer\GAL4NP0001-driven expression also leads to a severe decrease in lipid droplet-containing autophagosomes (dual LS2- and Atg8-positive puncta) in the adult midgut under control conditions, as compared to controls; the Scer\GAL4hs.PB-driven expression also abolishes the Ecc15 infection-induced lipid droplet autophagy (dual LS2- and Atg8-positive puncta) in the adult midgut observed in controls.
Starved third instar larvae expressing Atg1GD7149 under the control of Scer\GAL4Act5C.PI display a marked reduction in the area occupied by LysoTracker puncta in fat body cells compared to controls, furthermore the nucleation of Atg8 is abolished in these cells.
The clonal expression of Atg1GD7149 under the control of Scer\GAL4Act.PU results in a significant increase in the transverse cell area and a significant increase in relative mitochondrial content of midgut enterocytes during pupariation, as compared to controls.
Expression of Atg1GD7149 under the control of Scer\GAL4en-e16E does not induce either oxidative stress (monitored by levels of ROS) or cell death (visualized using [*]Casp3) in third instar larval wing discs.
Adults expressing Atg1GD7149 under the control of the cardioblast-specific Scer\GAL4tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.
Animals expressing Atg1GD7149 under the control of Scer\GAL4NP1 and analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut condensation is dramatically delayed in the mutants compared to controls.