FlyBase Allele Report: Dmel\Atg18a[GD12342]

General Information

Symbol

Dmel\Atg18a^GD12342

Species

D. melanogaster

Name

FlyBase ID

FBal0198784

Feature type

allele

Associated gene

Dmel\Atg18a

Associated Insertion(s)

Carried in Construct

P{GD12342}

Also Known As

Atg18-IR

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12342}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12342

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates neurodegenerative disease

modeled by Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

exacerbates mucopolysaccharidosis type IIIA

modeled by Sgsh^GD1578

(Webber et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^Act5C.PU

(Moy et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

embryonic/larval midgut | P-stage, with Scer\GAL4^NP0001

(Denton et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Atg18a^GD12342 under the control of Scer\GAL4^elav.PLu do not exhibit climbing defects compared to controls.

(Webber et al., 2018)

Expression of Atg18a^GD12342 under the control of Scer\GAL4^en-e16E does not induce either oxidative stress (monitored by levels of ROS) or cell death (visualized using [*]Casp3) in third instar larval wing discs.

(Liu et al., 2016)

Compared with wild-type, flies expressing Scer\GAL4^Act5C.PU>Atg18^GD12342 show greatly enhanced lethality after Rift Valley Fever Virus (RVFV) infection.

(Moy et al., 2014)

Adults expressing Atg18a^GD12342 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Animals expressing Atg18^GD12342 under the control of Scer\GAL4^NP1 and analysed at +4 hours relative to puparium formation show a significant delay in midgut histolysis compared to controls. At +12 hours relative to puparium formation, larval midgut condensation is dramatically delayed in the mutants compared to controls.

(Denton et al., 2009)

Flies expressing Atg18^GD12342 under the control of Scer\GAL4^Act are more sensitive to infection by Vesicular Stomatitis Virus than control flies, and succumb to the virus. There is no difference in the number of hemocytes in adult flies compared to controls.

(Shelly et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12342

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Atg18a^GD12342, Scer\GAL4^elav.PLu is an enhancer of abnormal locomotor behavior | adult stage | progressive phenotype of Scer\GAL4^elav.PLu, Sgsh^GD1578

(Webber et al., 2018)

Atg18a^GD12342, Scer\GAL4^en-e16E is an enhancer of increased cell death | third instar larval stage phenotype of Scer\GAL4^en-e16E, vtd^GL00522

(Liu et al., 2016)

Atg18a^GD12342, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Other

Statement

Reference

Atg18a^GD12342, Scer\GAL4^en-e16E, mad2^GD16427 has increased cell death | third instar larval stage phenotype

(Liu et al., 2016)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Atg18a^GD12342, Scer\GAL4^NP0001 has embryonic/larval midgut | P-stage phenotype, non-enhanceable by Decay^GD8484, Scer\GAL4^NP0001

(Denton et al., 2009)

Enhancer of

Statement

Reference

Atg18a^GD12342, Scer\GAL4^en-e16E is an enhancer of wing disc | third instar larval stage phenotype of Scer\GAL4^en-e16E, vtd^GL00522

(Liu et al., 2016)

Atg18a^GD12342, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{99.UAS.Tag:MYC,Tag:FLAG}, Zzzz\CTG^{rCUG.99.UAS.Tag:MYC,Tag:FLAG}

(Samaraweera et al., 2013)

Other

Statement

Reference

Atg18a^GD12342, Scer\GAL4^en-e16E, mad2^GD16427 has wing disc | third instar larval stage phenotype

(Liu et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The dramatic increase in apoptosis (visualized by [*]Casp3 or acridine orange staining) observed in larval wing discs upon expression of vtd^GL00522 driven by Scer\GAL4^en-e16E is increased further by co-expression of Atg18a^GD12342.

Co-expression of mad2^GD16427 with Atg18a^GD12342 under the control of Scer\GAL4^en-e16E results in a dramatic increase in cell death (visualized by [*]Casp3 staining) in third instar larval wing discs.

(Liu et al., 2016)

The delayed midgut histolysis phenotype seen in animals expressing Atg18^GD12342 under the control of Scer\GAL4^NP1 after puparium formation is not enhanced by co-expression of decay^GD8484.

(Denton et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22646

w¹¹¹⁸; P{GD12342}v22646/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12342

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Atg18^GD12342

Atg18a^GD12342

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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