UASt regulatory sequences drive expression of an inverted repeat.
Expression of BxGD1385 under the control of Scer\GAL4He.PZ does not lead to any changes in the haemocyte count while its expression under the control of Scer\GAL4He.PZ or Scer\GAL4lz-gal4 leads to a decrease in crystal cell numbers when compared to control larvae.
Expression of BxGD1385 under the control of Scer\GAL4elav-C155 does not lead to any easily observable egg phenotypes when compared to controls.
Expression of BxGD1385 under the control of Scer\GAL4pnr-MD237 leads to a reduction in the number of thoracic bristles compared to controls.
Eggs laid by females expressing BxGD1385 under the control of Scer\GAL4c204 or Scer\GAL4c323a but not Scer\GAL4c306 are smaller and translucent; appear collapsed; and their dorsal appendages are thin and shorter when compared to controls.
Expression of BxGD1385 under the control of Scer\GAL4slbo.2.6 does not lead to any changes in fecundity or border cell migration in the egg chamber of females when compared to controls.
Expression of BxGD1385 in muscle under the control of Scer\GAL4Mef2.PR has no effect on female fecundity or fertility. Expression of BxGD1385 in neurons under the control of Scer\GAL4elav-C155 results in highly reduced fecundity and fertility. Unhatched eggs are white in color, indicating a defect in fertilization. Ovulation is reduced, as is sperm release from the seminal receptacle. Oviposition defects are also seen: in contrast to control flies, Scer\GAL4elav-C155>BxGD1385 females lay eggs on the surface of the media rather than in it. Mature eggs accumulate in the ovary.
Expression of BxGD1385 under the control of Scer\GAL4elav-C155, but limited to either the pupal stages or adult stages using Scer\GAL80ts.αTub84B, does not cause any reproductive defects. However, expression during both stages results in significantly reduced fecundity and fertility.
Expression of BxGD1385 in the octopaminergic neurons under the control of Scer\GAL4Tdc2.PC does not affect either the fertility or fecundity of female flies.
Expression of BxGD1385 in the glutamatergic neurons under the control of Scer\GAL4VGlut.PD results in a partial reduction in fertility without affecting fecundity. Oviposition defects are also observed.
Expression of BxGD1385 in motor neurons under the control of Scer\GAL4futsch-C380 results in a significant reduction in fertility and fecundity. Oviposition defects are also observed.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 100% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in bristle morphology defects on the notum in 100% or 70-80% of the Scer\GAL4pnr-MD237 expression domain, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 30-40% or 40-50% of the Scer\GAL4pnr-MD237-expressing area of the notum, depending on the insertion line used.
BxGD1385/Scer\GAL4He.PZ rescues BxJ
BxGD1385/Scer\GAL4He.PZ partially rescues Bx1
Expression of BxGD1385 under the control of Scer\GAL4He.PZ fully rescues the decreased haemocyte count phenotype while it partially rescues the increased crystal cell number phenotype of Bx1 mutants.
