FB2025_01 , released February 20, 2025
Allele: Dmel\TTLL3BGD7234
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General Information
Symbol
Dmel\TTLL3BGD7234
Species
D. melanogaster
Name
FlyBase ID
FBal0199323
Feature type
allele
Associated gene
Dmel\TTLL3B
Associated Insertion(s)
Carried in Construct
P{GD7234}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD7234}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD7234
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing TTLL3BGD7234 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

      (Neely et al., 2010)

      50% of embryos expressing TTLL3BGD7234 under the control of Scer\GAL4da.G32 do not survive to adulthood.

      Almost all adult males expressing TTLL3BGD7234 under the control of Scer\GAL4da.G32 are sterile, with only 2.4% being able to generate offspring. Preindividualisation sperm cysts of mutant males appear similar to those of wild type. However, most mature cysts lack axoneme structures in the mutant males, while the mitochondrial derivatives are still present. In some cases, disorganised axonemes composed of singlet microtubules are seen. Rarely, cysts containing axonemes but with reduced or absent mitochondria are seen. Actin cones are scattered along the sperm tails.

      Expression of TTLL3BGD7234 under the control of Scer\GAL4da.G32 in the presence of Dcr-2Scer\UAS.cDa to enhance the transgenic RNAi effect results in 100% lethality.

      (Rogowski et al., 2009)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      CG11201GD7234
      TTLL3BGD7234
      Name Synonyms
      Secondary FlyBase IDs
        References (6)