FlyBase Allele Report: Dmel\RIOK1[GD8541]

General Information

Symbol

Dmel\RIOK1^GD8541

Species

D. melanogaster

Name

FlyBase ID

FBal0199513

Feature type

allele

Associated gene

Dmel\RIOK1

Associated Insertion(s)

Carried in Construct

P{GD8541}

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD8541}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

RIOK1

Associated Sequence Features

dsRNA-GD8541

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain glioma

modeled by Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013)

modeled by btl::Egfr^λ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Read et al., 2013)

modeled by Hsap\EGFR^Δ.UAS

(Read et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage

(Neumüller et al., 2011)

larval neuroblast | larval stage

(Neumüller et al., 2011)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Scer\GAL4^Ilp2.PR-mediated expression results in a significant reduction in pupal volume and the body weight of emerging adults compared to controls.

(Hasygar and Hietakangas, 2014)

Expression of RIOK1^GD8541 under the control of Scer\GAL4^repo.PU has no effect on third instar larval brain size.

(Read et al., 2013)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of ganglion mother cells in the larval brain.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing RIOK1^GD8541 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing RIOK1^GD8541 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

8541

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

RIOK1^GD8541, Scer\GAL4^Ilp2.PR has decreased body size | pupal stage phenotype, suppressible | partially by p53^JF02513, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

RIOK1^GD8541, Scer\GAL4^Ilp2.PR has decreased body size | pupal stage phenotype, suppressible | partially by p53^KK113065, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

Suppressor of

Statement

Reference

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of neoplasia | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

Other

Statement

Reference

Phenotype Manifest In

Suppressor of

Statement

Reference

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of glial cell | increased number | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

RIOK1^GD8541, Scer\GAL4^repo.PU is a suppressor of embryonic/larval brain | third instar larval stage phenotype of Hsap\EGFR^Δ.UAS, Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU

(Read et al., 2013)

Other

Statement

Reference

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK1^GD8541, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has embryonic/larval brain | third instar larval stage phenotype

(Read et al., 2013)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK1^GD8541, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has glial cell | third instar larval stage phenotype

(Read et al., 2013)

Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, RIOK1^GD8541, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS has neuron | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK1^GD8541, Scer\GAL4^repo.PU has embryonic/larval brain | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK1^GD8541, Scer\GAL4^repo.PU has glial cell | third instar larval stage phenotype

(Read et al., 2013)

Hsap\EGFR^Δ.UAS, RIOK1^GD8541, Scer\GAL4^repo.PU has neuron | third instar larval stage phenotype

(Read et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of RIOK1^GD8541 suppresses the increase in brain size and glial overgrowth seen when btl::Egfr^{Scer\UAS.T:λ\cI-DD} and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells.

(Read et al., 2013)

Xenogenetic Interactions

Statement

Reference

Expression of RIOK1^GD8541 suppresses the brain enlargement and glial overgrowth seen when Hsap\EGFR^Δ.Scer\UAS is expressed under the control of Scer\GAL4^repo.PU. There are few excess glia and remaining glial cells show abnormal development. The resulting brains are smaller in size than controls, due to a reduction in both glial and neuronal cells.

Expression of RIOK1^GD8541 suppresses the brain enlargement and glial overgrowth seen when Hsap\EGFR^Δ.Scer\UAS and Pi3K92E^{Scer\UAS.T:Hsap\MYC,T:Hsap\CAAX} are co-expressed under the control of Scer\GAL4^repo.PU.

(Read et al., 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v18526

w¹¹¹⁸; P{GD8541}v18526

Notes on Origin