Allele Dmel\kat-60L1^GD7435

General Information
Symbol	Dmel\kat-60L1^GD7435	Species	D. melanogaster
Name		FlyBase ID	FBal0199623
Feature type	allele	Associated gene	Dmel\kat-60L1

Map ( GBrowse )
Allele class
Mutagen	in vitro construct - RNAi, in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Dickson et al., 2007.7.18) in vitro construct - RNAi (Dickson et al., 2007.7.18)
Mutations Mapped to the Genome

Type Location Additional Notes References RNAi reagent :1..336
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an inverted repeat. (Dickson et al., 2007.7.18)
Carried in construct	P{GD7435} (Dickson et al., 2007.7.18, Lee et al., 2009, Tao and Rolls, 2011, Mummery-Widmer et al., 2009, Stewart et al., 2012)
Associated Sequence Features	dsRNA-GD7435
Cytology
Phenotypic Data
Phenotypic Class
	heat stress response defective, with Scer\GAL4^ppk.1.9 (Stewart et al., 2012) neuroanatomy defective, with Scer\GAL4^ppk.1.9 (Tao and Rolls, 2011) neuroanatomy defective \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009) stress response defective, with Scer\GAL4^ppk.1.9 (Stewart et al., 2012) viable, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) visible, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Phenotype Manifest In
	chaeta \| supernumerary, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009) dendrite, with Scer\GAL4^ppk.1.9 (Tao and Rolls, 2011) dendrite & dorsal multidendritic neuron ddaC \| pupal stage, with Scer\GAL4^ppk.PG (Lee et al., 2009) dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9 (Tao and Rolls, 2011) trichogen cell, with Scer\GAL4^pnr-MD237 (Mummery-Widmer et al., 2009)
Detailed Description
	Statement Reference Expression of kat-60L1[GD7435] in class IV da neurons, under the control of Scer\GAL4[ppk.1.9] results in a reduction in mechanical nocifensive response. Class IV neuron-specific RNAi knockdown of kat-60L1 through expression of kat-60L1[GD7435] under the control of Scer\GAL4[ppk.1.9] results in a severe delay in larval response to a high-temperature probe of 46[o]C. This insensitivity is maintained at a lower threshold temperature of 42[o]C, indicating an overall failure to respond to noxious temperatures. (Stewart et al., 2012) A reduction in the levels of kat-60L1 through expression of kat-60L1[GD7435] in ddaC dendrites under the control of Scer\GAL4[ppk.1.9] results in a strong reduction in the level of dendritic pruning. However, dendrite degeneration due to dendrite severing proceeds as in wild-type. (Tao and Rolls, 2011) The proximal dendrites remain intact for many ddaC neurons at 16 hours after puparium formation (APF) in animals expressing kat-60L1[GD7435] under the control of Scer\GAL4[ppk.PG], indicating a defect in dendritic severing during dendrite pruning (proximal dendrites can be seen disconnected from the ddaC soma at 5 hours APF in wild-type animals). (Lee et al., 2009) Expression under the control of Scer\GAL4[pnr-MD237] results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. Expression under the control of Scer\GAL4[pnr-MD237] results in bristle morphology defects on the notum in 0% or 20-30% of the Scer\GAL4[pnr-MD237] expression domain, depending on the insertion line used. (Mummery-Widmer et al., 2009)
External Data
Linkouts	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 7435
Interactions
Phenotypic Class
NOT Suppressor of
Statement Reference kat-60L1^GD7435, Scer\GAL4^ppk.1.9 is a non-suppressor of stress response defective phenotype of Crei\ChR2^{Scer\UAS.cHa.T:Avic\GFP-EYFP}, Scer\GAL4^ppk.1.9 (Stewart et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Nocifensive escape locomotion in response to blue-light stimulation is unaffected in Crei\ChR2[Scer\UAS.cHa.T:Avic\GFP-EYFP] flies expressing kat-60L1[GD7435] under the control of Scer\GAL4[ppk.1.9]. (Stewart et al., 2012)
Complementation & Rescue Data
Comments
Stocks ( 2 )
VDRC	v31598 w¹¹¹⁸; P{GD7435}v31598 v31599 w¹¹¹⁸; P{GD7435}v31599
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts
	Bristle Screen Database - A database for RNAi phenotypes in bristle and notum development • 7435
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	CG1193^GD7435 kat-60L1^GD7435
Name Synonym
Secondary FlyBase IDs

References ( 5 )
Research paper	Stewart et al., 2012, J. Neurosci. 32(34): 11631--11642 Katanin p60-like1 Promotes Microtubule Growth and Terminal Dendrite Stability in the Larval Class IV Sensory Neurons of Drosophila. [FBrf0219283] Tao and Rolls, 2011, J. Neurosci. 31(14): 5398--5405 Dendrites have a rapid program of injury-induced degeneration that is molecularly distinct from developmental pruning. [FBrf0213404] Lee et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(15): 6363--6368 Drosophila IKK-related kinase Ik2 and Katanin p60-like 1 regulate dendrite pruning of sensory neuron during metamorphosis. [FBrf0207681]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]

Allele Dmel\kat-60L1GD7435

Allele Dmel\kat-60L1^GD7435