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General Information
Symbol
Dmel\BaceGD5366
Species
D. melanogaster
Name
FlyBase ID
FBal0200099
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of BaceGD5366 under the control of Scer\GAL4αTub84B.PL results in complete lethality during the late-larval/early pupal stage.

Newly eclosed adults expressing BaceGD5366 under the control of Scer\GAL4Appl.G1a do not show a detectable mutant phenotype in the lamina. However, 100% of 4 week old flies have vacuoles in the lamina cortex and in the central brain.

Adults expressing BaceGD5366 under the control of Scer\GAL4GMR.PU show progressive degeneration of the lamina, with vacuolisation in the lamina cortex increasing over time. Although vacuolisation is present along the entire lamina cortex of 28 day old flies, vacuoles are not detected in the lamina neuropil or other brain areas. At 24-32 hours after eclosion, the first defects in the lamina cortex are detected close to the basement membrane. In 3 day old flies, gaps are seen in the row of glial cells in the subretinal layer, while the row of satellite and epithelial glia appear intact. Only a few glial cells are detectable in the subretinal layer in 3 week old flies and most of the remaining glia in the lamina cortex appear to be satellite glia.

Adults expressing BaceGD5366 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

External Data
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of either Appls.Scer\UAS.T:Ivir\HA1 or AppldAICD.Scer\UAS.T:Ivir\HA1 has no effect on the lamina degeneration phenotype seen in 28 day old flies expressing BaceGD5366 under the control of Scer\GAL4GMR.PU.

Co-expression of ApplScer\UAS.cCSa does not increase in general the lamina degeneration phenotype seen in 28 day old flies expressing BaceGD5366 under the control of Scer\GAL4GMR.PU, although vacuoles are occasionally seen in the lamina neuropil in these flies.

The lamina degeneration phenotype seen in 28 day old flies expressing BaceGD5366 under the control of Scer\GAL4GMR.PU is significantly suppressed by Appld.

Co-expression of Applsd.Scer\UAS dramatically enhances the lamina degeneration phenotype seen in 28 day old flies expressing BaceGD5366 under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

Co-expression of BaceScer\UAS.cCSa partially rescues the lethality caused by expression of BaceGD5366 under the control of Scer\GAL4αTub84B.PL.

Co-expression of BaceScer\UAS.cCSa reduces the vacuolisation in the lamina neuropil and cortex of 28 day old flies expressing BaceGD5366 under the control of Scer\GAL4GMR.PU to the levels seen in controls.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 1 )
Crossreferences
Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
BaceGD5366
CG13095GD5366
Name Synonyms
Secondary FlyBase IDs
    References (7)