FlyBase Allele Report: Dmel\Bace[GD5366]

General Information

Symbol

Dmel\Bace^GD5366

Species

D. melanogaster

Name

FlyBase ID

FBal0200099

Feature type

allele

Associated gene

Dmel\Bace

Associated Insertion(s)

Carried in Construct

P{GD5366}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5366}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5366

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain | progressive, with Scer\GAL4^Appl.G1a

(Bolkan et al., 2012)

adult subretinal glial cell | progressive, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

cell body rind of adult lamina | progressive, with Scer\GAL4^Appl.G1a

(Bolkan et al., 2012)

cell body rind of adult lamina | progressive, with Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Detailed Description

Statement

Reference

Expression of Bace^GD5366 under the control of Scer\GAL4^αTub84B.PL results in complete lethality during the late-larval/early pupal stage.

Newly eclosed adults expressing Bace^GD5366 under the control of Scer\GAL4^Appl.G1a do not show a detectable mutant phenotype in the lamina. However, 100% of 4 week old flies have vacuoles in the lamina cortex and in the central brain.

Adults expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU show progressive degeneration of the lamina, with vacuolisation in the lamina cortex increasing over time. Although vacuolisation is present along the entire lamina cortex of 28 day old flies, vacuoles are not detected in the lamina neuropil or other brain areas. At 24-32 hours after eclosion, the first defects in the lamina cortex are detected close to the basement membrane. In 3 day old flies, gaps are seen in the row of glial cells in the subretinal layer, while the row of satellite and epithelial glia appear intact. Only a few glial cells are detectable in the subretinal layer in 3 week old flies and most of the remaining glia in the lamina cortex appear to be satellite glia.

(Bolkan et al., 2012)

Adults expressing Bace^GD5366 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5366

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, enhanceable by Appl^sd.UAS, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

NOT Enhanced by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-enhanceable by Appl^s.UAS.Tag:HA, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-enhanceable by Appl^{dAICD.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-enhanceable by Appl^UAS.cCSa, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Suppressed by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible by Appl^d

(Bolkan et al., 2012)

NOT suppressed by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Appl^s.UAS.Tag:HA, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Appl^{dAICD.UAS.Tag:HA}, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Bace^GD5366, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, non-suppressible by Appl^UAS.cCSa, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has cell body rind of adult lamina | progressive phenotype, enhanceable by Appl^sd.UAS, Scer\GAL4^GMR.PU

(Bolkan et al., 2012)

Suppressed by

Statement

Reference

Bace^GD5366, Scer\GAL4^GMR.PU has cell body rind of adult lamina | progressive phenotype, suppressible by Appl^d

(Bolkan et al., 2012)

Other

Statement

Reference

Appl^UAS.cCSa, Bace^GD5366, Scer\GAL4^GMR.PU has lamina phenotype

(Bolkan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of either Appl^{s.Scer\UAS.T:Ivir\HA1} or Appl^{dAICD.Scer\UAS.T:Ivir\HA1} has no effect on the lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU.

Co-expression of Appl^{Scer\UAS.cCSa} does not increase in general the lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU, although vacuoles are occasionally seen in the lamina neuropil in these flies.

The lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU is significantly suppressed by Appl^d.

Co-expression of Appl^sd.Scer\UAS dramatically enhances the lamina degeneration phenotype seen in 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU.

(Bolkan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Bace^GD5366 is rescued by Scer\GAL4^GMR.PU/Bace^UAS.cCSa

(Bolkan et al., 2012)

Partially rescued by

Bace^GD5366 is partially rescued by Scer\GAL4^αTub84B.PL/Bace^UAS.cCSa

(Bolkan et al., 2012)

Comments

Co-expression of Bace^{Scer\UAS.cCSa} partially rescues the lethality caused by expression of Bace^GD5366 under the control of Scer\GAL4^αTub84B.PL.

Co-expression of Bace^{Scer\UAS.cCSa} reduces the vacuolisation in the lamina neuropil and cortex of 28 day old flies expressing Bace^GD5366 under the control of Scer\GAL4^GMR.PU to the levels seen in controls.

(Bolkan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v15541

w¹¹¹⁸; P{GD5366}v15541

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5366

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Bace^GD5366

Bace^RNAi#v15541

(Jiao et al., 2023)

CG13095^GD5366

Name Synonyms

Secondary FlyBase IDs

References (9)

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